Rice Science ›› 2017, Vol. 24 ›› Issue (5): 264-273.DOI: 10.1016/j.rsci.2017.02.002
• Orginal Article • Previous Articles Next Articles
Mohibul Alam Khan Md1,2, Haque Effi1, Chandra Paul Narayan3, Abdul Khaleque Md2, M. S. Al-Garni Saleh4, Rahman Mahfuzur5, Tofazzal Islam Md1()
Received:
2016-10-29
Accepted:
2017-02-24
Online:
2017-09-15
Published:
2017-08-31
Mohibul Alam Khan Md, Haque Effi, Chandra Paul Narayan, Abdul Khaleque Md, M. S. Al-Garni Saleh, Rahman Mahfuzur, Tofazzal Islam Md. Enhancement of Growth and Grain Yield of Rice in Nutrient Deficient Soils by Rice Probiotic Bacteria[J]. Rice Science, 2017, 24(5): 264-273.
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Strain | Source of isolation | Biochemical analysis | Molecular analysis | |||||
KOH test | Gram reaction | Catalase test | Oxidase test | Accession No. | Closest strain from GenBank | Sequence similarity (%) | ||
BRRh-1 | Surface sterilized seeds of Kalijira | + | - | + | + | KF937789 | P. saccharolyticum RD_AZIDI_13 | 99 |
BRRh-2 | Rhizosphere soil of Paijam | + | - | + | + | KF921289 | Burkholderia sp. MSh1 | 99 |
BRRh-3 | Rhizoplane of BINA dhan-7 | + | - | + | + | KF974366 | Burkholderia sp. SAP27_1 | 100 |
BRRh-4 | Rhizosphere soil of BINA dhan-7 | + | - | + | + | KF921290 | Burkholderia sp. SAP53_1 | 99 |
BRRh-5 | Rhizosphere soil of Paijam | + | - | + | + | KF937787 | P. aeruginosa 147 | 99 |
BRRh-6 | Rhizoplane of BINA dhan-7 | + | - | + | + | KF937788 | P. aeruginosa 147 | 99 |
‘+’ indicates positive response; ‘-’ indicates negative response. |
Table 1 Biochemical and molecular characterization of rice probiotic bacteria isolated from different sources.
Strain | Source of isolation | Biochemical analysis | Molecular analysis | |||||
KOH test | Gram reaction | Catalase test | Oxidase test | Accession No. | Closest strain from GenBank | Sequence similarity (%) | ||
BRRh-1 | Surface sterilized seeds of Kalijira | + | - | + | + | KF937789 | P. saccharolyticum RD_AZIDI_13 | 99 |
BRRh-2 | Rhizosphere soil of Paijam | + | - | + | + | KF921289 | Burkholderia sp. MSh1 | 99 |
BRRh-3 | Rhizoplane of BINA dhan-7 | + | - | + | + | KF974366 | Burkholderia sp. SAP27_1 | 100 |
BRRh-4 | Rhizosphere soil of BINA dhan-7 | + | - | + | + | KF921290 | Burkholderia sp. SAP53_1 | 99 |
BRRh-5 | Rhizosphere soil of Paijam | + | - | + | + | KF937787 | P. aeruginosa 147 | 99 |
BRRh-6 | Rhizoplane of BINA dhan-7 | + | - | + | + | KF937788 | P. aeruginosa 147 | 99 |
‘+’ indicates positive response; ‘-’ indicates negative response. |
Fig. 1. Neighbor-joining phylogenetic tree based on 16S rRNA gene sequence.^The significance of each branch is indicated by a bootstrap value based on 1 000 replications. ^T indicates type strain.
Isolate | Phosphate solubilization | IAA production | |
---|---|---|---|
PSI in agar assay | PSI in broth assay | ||
BRRh-1 | - | 74.0 ± 1.0 | 25.0 ± 0.1 |
BRRh-2 | 3.0 ± 0.1 | 83.0 ± 2.0 | 34.0 ± 0.1 |
BRRh-3 | 4.0 ± 0.0 | 86.0 ± 1.0 | 23.0 ± 1.0 |
BRRh-4 | 5.0 ± 0.1 | 92.0 ± 2.0 | 9.0 ± 1.0 |
BRRh-5 | 4.0 ± 0.1 | 87.0 ± 1.0 | 8.0 ± 1.0 |
BRRh-6 | 4.0 ± 0.1 | 85.0 ± 2.0 | - |
PSI, Phosphate solubilization index; IAA, Indole-3-acetic acid. PSI = (Halo zone + Colony diameter) / Colony diameter. |
Table 2 Plant growth-promoting traits of rice probiotic bacteria (Mean ± SE, n = 3). µg/mL
Isolate | Phosphate solubilization | IAA production | |
---|---|---|---|
PSI in agar assay | PSI in broth assay | ||
BRRh-1 | - | 74.0 ± 1.0 | 25.0 ± 0.1 |
BRRh-2 | 3.0 ± 0.1 | 83.0 ± 2.0 | 34.0 ± 0.1 |
BRRh-3 | 4.0 ± 0.0 | 86.0 ± 1.0 | 23.0 ± 1.0 |
BRRh-4 | 5.0 ± 0.1 | 92.0 ± 2.0 | 9.0 ± 1.0 |
BRRh-5 | 4.0 ± 0.1 | 87.0 ± 1.0 | 8.0 ± 1.0 |
BRRh-6 | 4.0 ± 0.1 | 85.0 ± 2.0 | - |
PSI, Phosphate solubilization index; IAA, Indole-3-acetic acid. PSI = (Halo zone + Colony diameter) / Colony diameter. |
Fig. 2. Phosphate solubilization assay of BRRh-2 (A), BRRh-3 (B), BRRh-4 (C), BRRh-5 (D) and BRRh-6 (E) on National Botanical Research Institute’s phosphate agar plate containing tricalcium phosphate (TCP). ^Halozone indicates inorganic phosphate solubilization ability of the isolates.
Fig. 3. Changes of the bacterial growth represented by optical density at 595 nm (A-F) and pH value of culture medium (G-L) with time of inoculation in National Botanical Research Institute’s phosphate growth medium (Mean ± SE, n = 3).
Fig. 4. Effects of BRRh-4 and BRRh-5 along with different fertilizer levels on various growth parameters of BRRI dhan-29.^Values (Mean ± SE, n = 3) followed by the same letter(s) in the same graph did not differ significantly at the 0.05 level by the LSD test.
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