Development and Application of CRISPR/Cas System in Rice

doi:10.1016/j.rsci.2019.01.001

摘要/Abstract

关键词: rice, CRISPR, Cas, genome editing, protospacer adjacent motif

. [J]. Rice Science, 2019, 26(2): 69-76.

图/表 1

参考文献 63

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Property/Tool	PAM	Target gene	Reference
CRISPR/SpCas9	NGG	OsPDS, OsBADH2, Os02g23823, OsMPK2	Shan et al, 2013
		OsMPK5	Xie et al, 2013
		OsPDS, OsPMS3, OsEPSPS, OsDERF1, OsMSH1, OsMYB5, OsMYB1, OsROC5, OsSPP, OsYSA	Zhang et al, 2014
		OsFTL11, Os07g0261200, Os02g0700600, OsGSTU, OsMRP15, OsWaxy	Ma et al, 2015
		OsPDS, Os02g23823, OsMPK2	Wang et al, 2015
		OsPDS, OsYSA, OsDEP1	Tang et al, 2016
		OsBADH2, OsDEP1, OsGn1a, OsGS3, OsGW2, OsHd1, OsEP3, OsLPA1	Shen et al, 2017a
		OsMPK5, OsPDS, OsMPK2, OsMPK1	Ding et al, 2018
	NAG	OsRAD51A, OsDMC1, OsNAL1, OsLPA1, OsLG1, OsGL1-1	Meng et al, 2018
CRISPR/SpCas9-VQR	NGA	OsNAL1, OsLPA1, OsLG1, OsGL1-1	Hu et al, 2016
CRISPR/SpCas9-VRER	NGCG	OsNAL1, OsLG1, OsGL1-1	Hu et al, 2016
CRISPR/SaCas9	NNGRRT	OsDL, OsCYP72A33, OsCYP72A32, OsVIP1, OsVIP1-like gene	Kaya et al, 2016
CRISPR/FnCpf1	TTN	OsDL, OsALS	Endo et al, 2016
		OsRLK-798, OsRLK-799, OsRLK-802, OsRLK-803	Wang et al, 2017b
		OsPDS, OsMPK2, OsMPK5	Ding et al, 2018
CRISPR/LbCpf1	TTTN	OsBEL-230, OsBEL-240, OsBEL-250, OsBEL-260	Wang et al, 2017b
		OsPDS, OsDEP1, OsROC5	Tang et al, 2017
		OsPDS, OsBEL	Xu et al, 2017
		OsNAL1, OsLG1	Hu et al, 2017
		OsPDS, OsMPK2, OsMPK5	Ding et al, 2018
CRISPR/AsCpf1	TTTN	OsPDS, OsDEP1, OsROC5	Tang et al, 2017
CRISPR/AsCpf1	TTTN	OsNAL1, OsLG1	Hu et al, 2017
CRISPR/LbCpf1-RR	TYCV	OsPDS, OsSBEIIb	Li et al, 2018b
	TYCV	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
	CCCC	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/LbCpf1-RVR	TATG	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/FnCpf1-RR	CCCC	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/FnCpf1-RR	TYCY	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/FnCpf1-RVR	TATG	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
PAM, Protospacer adjacent motif. N represents A/T/G/C base. R represents A/G base. Y represents T/C base. V represents A/C/G base.

Property/Tool	PAM	Target gene	Reference
CRISPR/SpCas9	NGG	OsPDS, OsBADH2, Os02g23823, OsMPK2	Shan et al, 2013
		OsMPK5	Xie et al, 2013
		OsPDS, OsPMS3, OsEPSPS, OsDERF1, OsMSH1, OsMYB5, OsMYB1, OsROC5, OsSPP, OsYSA	Zhang et al, 2014
		OsFTL11, Os07g0261200, Os02g0700600, OsGSTU, OsMRP15, OsWaxy	Ma et al, 2015
		OsPDS, Os02g23823, OsMPK2	Wang et al, 2015
		OsPDS, OsYSA, OsDEP1	Tang et al, 2016
		OsBADH2, OsDEP1, OsGn1a, OsGS3, OsGW2, OsHd1, OsEP3, OsLPA1	Shen et al, 2017a
		OsMPK5, OsPDS, OsMPK2, OsMPK1	Ding et al, 2018
	NAG	OsRAD51A, OsDMC1, OsNAL1, OsLPA1, OsLG1, OsGL1-1	Meng et al, 2018
CRISPR/SpCas9-VQR	NGA	OsNAL1, OsLPA1, OsLG1, OsGL1-1	Hu et al, 2016
CRISPR/SpCas9-VRER	NGCG	OsNAL1, OsLG1, OsGL1-1	Hu et al, 2016
CRISPR/SaCas9	NNGRRT	OsDL, OsCYP72A33, OsCYP72A32, OsVIP1, OsVIP1-like gene	Kaya et al, 2016
CRISPR/FnCpf1	TTN	OsDL, OsALS	Endo et al, 2016
		OsRLK-798, OsRLK-799, OsRLK-802, OsRLK-803	Wang et al, 2017b
		OsPDS, OsMPK2, OsMPK5	Ding et al, 2018
CRISPR/LbCpf1	TTTN	OsBEL-230, OsBEL-240, OsBEL-250, OsBEL-260	Wang et al, 2017b
		OsPDS, OsDEP1, OsROC5	Tang et al, 2017
		OsPDS, OsBEL	Xu et al, 2017
		OsNAL1, OsLG1	Hu et al, 2017
		OsPDS, OsMPK2, OsMPK5	Ding et al, 2018
CRISPR/AsCpf1	TTTN	OsPDS, OsDEP1, OsROC5	Tang et al, 2017
CRISPR/AsCpf1	TTTN	OsNAL1, OsLG1	Hu et al, 2017
CRISPR/LbCpf1-RR	TYCV	OsPDS, OsSBEIIb	Li et al, 2018b
	TYCV	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
	CCCC	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/LbCpf1-RVR	TATG	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/FnCpf1-RR	CCCC	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/FnCpf1-RR	TYCY	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
CRISPR/FnCpf1-RVR	TATG	OsROC5, OsPDS, OsDEP1	Zhong et al, 2018
PAM, Protospacer adjacent motif. N represents A/T/G/C base. R represents A/G base. Y represents T/C base. V represents A/C/G base.

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