Comparison of Morphological and Genetic Differentiations in Filial Generation of Cross Between Indica and Japonica Rice

doi:10.1016/S1672-6308(08)60108-0

Abstract

Abstract: A recombinant inbred line (RIL) population of F8 and F9 generations derived from a cross between a typical indica rice (Qishanzhan) and a typical japonica rice (Akihikari) was used to study the difference between morphological differentiation based on phenotype characters and genetic differentiation using indica and japonica specific SSR markers, and to evaluate the relationship between vascular bundle characters and morphological and genetic differentiations. The results showed that the frequency distributions of morphological and genetic differentiations were all inclined to japonica type in the filial generation. The population was more inclined to japonica type based on genetic differentiation than on morphological differentiation. The consistent degrees of classification based on the Cheng’s index, the ratio of large vascular bundle number to small vascular bundle number in panicle neck (RLSVB) and the ratio of large vascular bundle number in the second internode from the top to that in the panicle neck (RLVB) were all about 50% compared with the genetic differentiation, and the consistent degree of the total scores of the Cheng’s index combined with the vascular bundle number ratios was significantly increased to about 80% compared with the genetic differentiation. Therefore, the vascular bundle characters could be used as a helpful supplement for subspecies classification.

Key words: rice, recombinant inbred line, Cheng's index, vascular bundle characters, simple sequence repeat marker, subspecies classification method

MAO Ting, XU Hai, XU Quan, GUO Yan-hua, ZHU Chun-jie, CHEN Kai, WANG Jia-yu, XU Zheng-jin. Comparison of Morphological and Genetic Differentiations in Filial Generation of Cross Between Indica and Japonica Rice[J]. RICE SCIENCE, 2010, 17(1): 82-86 .

References

1 Sun C Q, Wang X K, Li Z C, Yoshimura A Iwata N. Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers. Theor Appl Genet, 2001, 102: 157–162.
2 Li R H, Xu C G, Sun C Q, Wang X K, Xu C G. Genotype difference degree and classification for cultivated rice. Acta Agron Sin, 1999, 25(4): 518–526. (in Chinese with English abstract)
3 Zhou H, Glaszmann J C, Cheng K S, Shi X Q. Comparison of the classification method of cultivated rice. Chinese J Rice Sci, 1988, 2(1): 1–7. (in Chinese with English abstract)
4 Cheng K S. Discrimination of indica-japonica subspecies in Asia Cultivated Rice. Kunming: Yunnan Science and Technology Press, 1993: 1–23. (in Chinese)
5 Wang X K, Li R H, Sun C Q, Li Z C, Cai H W, Sun X L. Recognition and classification of the hybrid rice between subspecies in Asia cultivated rice. Chinese Sci Bull, 1997, 42(24): 2596–2603. (in Chinese)
6 Ling Q H, Cai J Z, Su Z F. Research in the relations between rice neck-panicle vascular and bundles and spike characteristics and its applications. Jiangsu Agric Res, 1982, 3(3): 7–16. (in Chinese with English abstract)
7 Huang H. The relations between the upmost internode organization and number of spikelets in rice. Acta Agron Sin, 1998, 24(3): 193–200. (in Chinese with English abstract)
8 Xu Z J, Chen W F, Zhang B L, Peng Y C, Zhang Y G. Type difference and genetic study on the upmost internode vascular bundles in rice. Acta Agron Sin, 1996, 22(2): 167–172. (in Chinese with English abstract)
9 Sano R, Morishima H. Indica-japonica differentiation of rice cultivars viewed from variation in key characters and isozymes with special reference to landraces from the Himalayan hilly areas. Theor Appl Genet, 1992, 84: 266–274.
10 Zhang Q F, Saghai Maroof M A, Lu T Y, Shen B Z. Genetic diversity and differentiation of indica and japonica rice detected by RFLP analysis. Theor Appl Genet, 1992, 83: 495– 499.
11 Cai X X, Liu J, Qiu Y Q, Zhao W, Song Z P, Lu B L. Differentiation between indica rice 93-11 and japonica rice revealed by difference of DNA insertion/deletion of indica rice and japonica rice Nipponbare. J Fudan Univ: Nat Sci, 2006, 45(3): 309–315. (in Chinese with English abstract)
12 Ohmido N, Kijima K, Akiyama Y, de Jong J H, Fukui K. Quantification of total genomic DNA and selected repetitive sequences reveals concurrent changes in different DNA families in indica and japonica rice. Mol Gen Genet, 2002, 263: 388–394.
13 Bautista N S, Solis R, Kamijima O, Ishii T. RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice. Gen Genet Sysm, 2001, 76: 71–79.
14 Ni J J, Colowit P M, Mackill D J. Evaluation of genetic diversity in rice subspecies using microsatellite markers. Crop Sci, 2002, 42: 601–607.
15 Wang S W, Liu X, Wang Y, Xu C G, Shi L L, Zhang X, Ding D L. Genome polymorphisms between indica and japonica revealed by RFLP. Sci Agric Sin, 2006, 39(5): 1038–1043. (in Chinese with English abstract)
16 Chen L Y, Xiong W, Yang J H, Xiao Y H, Liu G H, Tang W B, Sun C Q, Chen L, Zhu Z F. Relationship between the extent of genetic differentiation in parental lines and seed setting percentage stability in intersubspecific hybrid rice. J Hunan Agric Univ, 2002, 28(2): 89–92. (in Chinese with English abstract)
17 Mei H W, Li Z K, Wang Y P, Yu X Q, Zhong D B, Luo L J, Ying C S. The indica-japonica classification of ‘Lemont/ Teqing’ recombinant inbred lines (RILs) using Cheng’s index. Chinese J Rice Sci, 1997, 11(4): 193–197. (in Chinese with English abstract)
18 He H H, Luo X J, Zhu C L, He X P, Fu J L, Liu Y B, Wu Q, Hua Y J, Xu B G, Wang X K. SSR classification between sterile line and restorer line in hybrid rice. Acta Agron Sin, 2006, 32(2): 169–175. (in Chinese with English abstract)
19 Zhang D L, Zhang D L, Wei X H, Qi Y W, Wang M X, Sun J L, Ding L, Tang S X, Qiu Z E, Cao Y S, Wang X K, Li Z C. Genetic structure and genetic diversities of Guizhou cultivated rice. Chinese Sci Bull, 2006, 51(23): 2747–2754. (in Chinese)
20 Zhu C J, Xu H, Guo Y H, Wang J Y, Liu H G, Xu Z J. Discrimination of the subspecies in indica-japonica recombinant inbred lines and the variation of vascular bundles. Chinese J Rice Sci, 2007, 21(6): 619–624. (in Chinese with English abstract)
21 Zhu Z F, Sun C Q, Fu Y C, Zhang P J, Wang X K. Genetic diversities revealed by comparing Asia cultivated rice and common wild rice by SSR markers. Sci Agric Sin, 2002, 35(12): 1437–1441. (in Chinese with English abstract)
22 Zhang H L, Li Z C, Liao D Q, Liu X, Zeng Y W, Shen S Q, Mu P, Yang Z Y, Wang X K. Microsatellite analysis for core collection of Yunnan rice landraces. J Agric Biotech, 2003, 11(2): 131–139. (in Chinese with English abstract)
23 Cheng S H, Mao C Z, Zhan X D, Si H M, Sun Z X. The construction of DH populations and RIL populations for indica-japonica and the differentiation of indica and japonica. Chinese J Rice Sci, 2001, 15(4): 257–260. (in Chinese with English abstract)
24 Fan Y Y, Zhuang J Y, Wu J L, Sun B L, Zheng K L. SSLP based identification of subspecies in rice (Oryza sativa L.). Hereditas(Beijing), 2000, 22(6): 392–394. (in Chinese with English abstract)
25 Guo Y H, Xu H, Zhu C J, Xu Z J. Variations about subspecies characteristics and relationship between subspecies characteristics and vascular bundles characteristics in filial generation of cross between indica and japonica. Seeds, 2008(3): 1–4. (in Chinese)
26 Wang Z Y, Tanksley S D. Restriction fragment length polymorphism in Oryza sativa L. Genome, 1989, 32: 1113– 1118.
27 Glaszmann J C. Isozymes and classification of Asian rice. Theor Appl Genet, 1987, 74: 21–30.

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