Purification and Analysis of Abscisic Acid-Specifically-Inducible Proteins from Rice Callus

Abstract

Abstract: Two ABA-specifically-inducible proteins from rice callus were isolated and purified by precipitation with 65-100% saturated(NH4)2SO4, followed by the DEAE-sepharose, TSK-gel, and two-dimension electrophoresis. Iso-electric points (pI) of the proteins with the same molecular mass (24.5 kD) were 6.1 and 6.9, respectively. The Western blot analysis indicated that the proteins expressed in different tissues were obviously different. The A1 (pI 6.1) protein was only detected in calli treated with ABA and seed embryos (SE).However, the A2 (pI 6.9) protein was found not only in the calli treated with ABA and SE, but also in the white dry callus. Thus it suggested that the two proteins might play some important roles in the processes of seed embryo (or somatic embryo) formation.

Key words: rice, callus, abscisic acid, specifically-inducible protein, purification, expression

JIANG Hua , XU Zheng-jun , GAO Xiao-ling . Purification and Analysis of Abscisic Acid-Specifically-Inducible Proteins from Rice Callus[J]. RICE SCIENCE, 2007, 14(2): 111-117 .

References

1 Higuchi N, Maeda E. Enhanced plant regeneration in rice callus cultures following abscisic acid treatment. Japan J Crop Sci, 1990, 59: 359-368.
2 Swiatek A, Lenjou M, van Bockstaele D, Inze D, van Onckelen H. Differential effect of jasmonic acid and abscisic acid on cell cycle progression in tobacco by-2 cells. Plant Physiol, 2002, 128: 201-211.
3 Gao S J, Chen R K, Ma H M. Factors influencing the regeneration frequency of mature embryo derived callus in Hsien rice cultivars (Oryza sativa L.). Acta Agron Sin, 2004, 30(12): 1254-1258. (in Chinese with English abstract)
4 Jiang H, Chen J, Gao X L, Wan J, Wang P R, Wang X D, Xu Z J. Effect of ABA on rice callus and development of somatic embryo and plant regeneration. Acta Agron Sin, 2006, 32(9): 1379-1383. (in Chinese with English abstract)
5 Jiang H, Gao X L, Wan J, Chen J, Wang X D, Wang X D, Xu Z J. Effect of abscisic acid on saccharide metabolism and related enzyme activities in rice callus. Chinese J Rice Sci, 2006, 20(4): 406-410. (in Chinese with English abstract)
6 Xu Z J, Fujino K, Furuya C. Plant regeneration and novel protein synthesis in a long-term cultured callus of rice in response to abscisic acid. Japan J Crop Sci, 1995, 64(1): 109-114.
7 Qu L Q, Wei X L, Satoh H, Kumamaru T, Ogawa M. Seed storage glutelin α-2 subunit lacking mutants in rice. Acta Bot Sin, 2001, 43(11): 1167-1171.
8 Laemmli U K. Cleabage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680-685.
9 Lamb C, Dixon R A. The oxidative burst in plant disease resistance. Annu Rev Plant Mol Biol, 1997, 48: 251-275.
10 Hu Y J, Wei L, Liu S N, Ding Y. Analysis of male sterility-related proteins of young panicle in rice (Oryza sativa L.) by two-dimensional electrophoresis. J Wuhan Univ: Nat Sci, 2001, 22: 2103-2109.
11 Li S P, Deng Y Q, Wang X C, Wang Y P, Wang J Z. Melatonin protects SH-SY5Y neuroblastoma cells from calyculin A-induced neurofilament impairment and neurotoxicity. J Pineal Res, 2004, 36(3): 186-192.
12 Sambrook J, Fritsh E F, Manniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY: CSHL Press, 1989.
13 Yazaki J, Shimatani Z, Hashimoto A, Nagata Y, Fujii F, Kojima K, Suzuki K, Taya T, Tonouchi M, Nelson C. Transcriptional profiling of genes responsive to abscisic acid and gibberellin in rice: Phenotyping and comparative analysis between rice and Arabidopsis. Physiol Genomics, 2004, 17: 87-100.
14 Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H. High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.). Plant Biotech, 2005, 22(1): 47–50.
15 Chandler P M. Gene expression regulated by ABA and its relation to stress tolerance. Annu Rev Plant Physiol Plant Mol Biol, 1994, 45: 113.
16 Mao G L, Ha X F, Sun J, Xu X. Researches on the content change of Lycium Barbarum L. callus soluble proteins under NaCl stress. J Ningxia Univ: Nat Sci, 2005, 26(1): 64-66. (in Chinese with English abstract)
17 Toyofuku K, Loreti E, Vernieri P, Alpi A, Perata P, Yamaguchi J. Glucose modulates the abscisic acid- inducible Rab16A gene in cereal embryos. Plant Mol Biol, 2000, 42: 451-460.
18 Neill S J, Horgan R, Rees A F. Seed development and vivipary in Zea mays L. Planta, 1987, 171: 358-364.
19 Jensen A B, Goday A, Figueras M, Jessop A C, Pages M. Phosphorylation mediates the nuclear targeting of the maize Rab17 protein. Plant J, 1998, 13: 691-697.
20 Mundy J, Chua N H. Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J, 1988, 7: 2279-2286.

[1]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[2]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[3]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[4]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[5]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[6]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[7]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[8]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[9]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[10]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[11]	Jiang Changjie, Liang Zhengwei, Xie Xianzhi. Priming for Saline-Alkaline Tolerance in Rice: Current Knowledge and Future Challenges [J]. Rice Science, 2023, 30(5): 417-425.
[12]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[13]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[14]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.
[15]	Lu Xuedan, Li Fan, Xiao Yunhua, Wang Feng, Zhang Guilian, Deng Huabing, Tang Wenbang. Grain Shape Genes: Shaping the Future of Rice Breeding [J]. Rice Science, 2023, 30(5): 379-404.