Development of Chromosomal Segment Substitution Lines from a Backcross Recombinant Inbred Population of Interspecific Rice Cross

Abstract

Abstract: A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B // Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped on planthopper resistance and yield traits. A linkage map consisting of 119 DNA markers and spanned for 1188 cM over the 12 rice chromosomes was constructed. Thirty-two chromosomal segment substitution lines were selected based on the percentage of Xieqingzao B allele at marker loci. These lines are of great potential for gene mapping and alien gene introgression.

Key words: alien introgression, DNA marker, Dongxiang wild rice, chromosomal segment substitution line

CHEN Jie , Hafeez Ur Rahman Bughio , Chen Da-zhou , LIU Guang-jie , Zheng Kang-le , Zhuang Jie-yun . Development of Chromosomal Segment Substitution Lines from a Backcross Recombinant Inbred Population of Interspecific Rice Cross[J]. RICE SCIENCE, 2006, 13(1): 15-21 .

References

1 Multani D S, Jena K K, Brar D S, Reyes B D, Angeles E R, Khush G S. Development of monosomic alien addition lines and introgression of genes from Oryza australiensis Domin.to cultivated rice O. sativa L. Theor Appl Genet, 1994, 88: 102-109.
2 Brar D S, Khush G S. Alien introgression in rice. Plant Mol Biol, 1997, 35: 35-47.
3 Huang Z, He G, Shu L, Li X, Zhang Q. Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet, 2001, 102: 929-934.
4 Renganayaki K, Fritz A K, Sadasivam S, Pammi S, Harrington S E, McCouch S R, Kumar S M, Reddy A S. Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from O. officinalis into cultivated rice, O. sativa. Crop Sci, 2002, 42: 2112-2117.
5 Gu K, Tian D, Yang F, Wu L, Sreekala C, Wang D, Wang G L, Yin Z. High-resolution genetic mapping of Xa27 (t). a new bacterial blight resistance gene in rice. Oryza sativa L. Theor Appl Genet, 2004, 108: 800-807.
6 Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics, 1995, 141: 1147-1162.
7 Li J, Thomson M, McCouch S R. Fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3. Genetics, 2004, 168: 2187- 2195
8 Monforte A J, Tanksley S D. Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L.esculentum genetic background: A tool for gene mapping and gene discovery. Genome, 2000, 43: 803-813.
9 Doi K, Iwata N, Yoshimura Y. The construction of chromosome substitution lines of African rice (Oryza glaberrima Stued.) in the background of japonica rice (O. sativa L.). Rice Genet Newsl, 1997, 14: 39-41.
10 Aida Y, Tsunematsu H, Doi K, Yoshimura A. Development of a series of introgression lines of japonica in the background of indica rice. Rice Genet Newsl, 1997, 14; 41-43.
11 Kurakazu T, Sobrizal, Ikeda K, Sanchez P L, Doi K, Angeles E R, Khush G S, Yoshimura A. Oryza meridionalis chromosomal segment introgression lines in cultivated rice, O. sativa L. Rice Genet Newsl, 2001, 18: 81-82.
12 Li W T, Zeng R Z, Zhang Z M, Zhang G Q. Analysis of introgressed segments in near isogenic lines for F1 pollen sterility in rice. Chinese J Rice Sci, 2003, 17(2): 95-99
13 Jiang L, Cao Y J, Wang C M, Zhai H Q, Wan J M, Yoshimura A. Detection and analysis of QTL for seed dormancy in rice (Oryza sativa L.) using RIL and CSSL population. Acta Genet Sin, 2003, 30(5): 453-458.
14 Ishimaru K. Identification of a locus increasing rice yield and physiological analysis of its function. Plant Physiol, 133: 1083-1090.
15 Inukai T, Zeigler R S, Sarkarung S, Bronson M, Dung L V, Kinoshita T, Nelson R J. Development of pre-isogenic lines for rice blast-resistance by marker-aided selection from a recombinant inbred population. Theor Appl Genet, 1996, 93: 560-567.
16 Lu Y J, Zheng K L. A simple method for isolation of rice DNA. Chinese J Rice Sci, 1992, 6: 47-48.
17 Sambrook J, Fritch E F, Maniatis T. Molecular cloning: a laboratory manual. 2nd edn. New York: Cold Spring Harbor Laboratory Press, 1989.
18 Shi Y F, Ying J Z, Wang L, Zhu Z W, Zhuang J Y. Screening SSR markers for rice variety identification. Chinese J Rice Sci, 2005, 19(3): 195-201.
19 Lander E S, Green P, Abrahamson J, Barow A, Daly M J, Lincoln S E, Newberg L. Mapmaker: an interactive computer program for constructing primary genetic maps of experimental and natural populations. Genomics, 1987, 1: 174-181.
20 Manly K F, Cudmore R H Jr, Meer J M. Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome, 2001, 12: 930-932.
21 Lin S Y, Sasaki T, Yano M. Mapping quantitative trait loci controlling seed dormancy and heading date in rice Oryza sativa L., using backcross inbred lines. Theor Appl Genet, 1998, 96: 997-1003.
22 Kosambi D D. The estimation of map distance from recombination values. Ann Eugen, 1944, 12: 172-175.
23 van Berloo R. GGT: Graphical GenoTyping. Wageningen, Netherlands: Laboratory of Plant Breeding, Wageningen University & Research Centre, 1999.
24 Tanksley S D, Nelson J C. Advanced backcross QTL analysis: a method for simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet, 1996, 92: 191-120.
25 Causse M A, Fulton T M, Cho Y G, Ahn S N, Chunwongse J, Wu K S, Xiao J H, Yu Z H, Ronald P C, Harringtom S E, Second G, McCouch S R, Tanksley S D. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics, 1994, 138: 1251-1274.
26 Aluko G, Martinez C, Tohme J, Castano C, Bergman C, Oard J H. QTL mapping of grain quality traits from the interspecific cross Oryza sativa × O. glaberrima. Theor Appl Genet, 2004, 109: 630-639.
27 Lorierx M, Goffinet B, Perrier X, Gonzalez D, Lanaud C. Maximum-likelihood models for mapping genetic markers showing segregation distortion: Ⅰ. Backcross populations. Theor Appl Genet, 1995, 90: 73-80.
28 Thomson M J, Tai T H, McClung A M, Lai X H, Hinga M E, Lobos K B, Xu Y, Martinez C P, McCouch S R. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107: 479-493.
29 Yamamoto T, Kuboki Y, Lin S Y, Sasaki T, Yano M. Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single mendelian factors. Theor Appl Genet, 1998, 97: 37-44.

[1]	Nie Yuanyuan, Xia Hui, Ma Xiaosong, Lou Qiaojun, Liu Yi, Zhang Anling, Cheng Liang, Yan Longan, Luo Lijun. Dissecting Genetic Basis of Deep Rooting in Dongxiang Wild Rice [J]. Rice Science, 2022, 29(3): 277-287.
[2]	Weidong Qi, Hongping Chen, Zuozhen Yang, Biaolin Hu, Xiangdong Luo, Bing Ai, Yuan Luo, Yu Huang, Jiankun Xie, Fantao Zhang. Systematic Characterization of Long Non-Coding RNAs and Their Responses to Drought Stress in Dongxiang Wild Rice [J]. Rice Science, 2020, 27(1): 21-31.
[3]	Fantao Zhang, Yuan Luo, Meng Zhang, Yi Zhou, Hongping Chen, Biaolin Hu, Jiankun Xie. Identification and Characterization of Drought Stress- Responsive Novel microRNAs in Dongxiang Wild Rice [J]. Rice Science, 2018, 25(4): 175-184.
[4]	SHEN Xian-hua, YAN Song, HUANG Ren-liang, ZHU Shan, XIONG Hong-liang, SHEN Lin-jun. Development of Novel Cytoplasmic Male Sterile Source from Dongxiang Wild Rice (Oryza rufipogon) [J]. RICE SCIENCE, 2013, 20(5): 379-382.
[5]	Venkateswarlu YADAVALLI1, 2, Gajendra P. NARWANE2, M. S. R. KRISHNA3, Nagarajan POTHI2, Bharathi MUTHUSAMY4. Tagging of Brown Planthopper Resistance Genes in F2s of IR50 × Ptb33 of Rice by Using Bulked Segregant Analysis [J]. RICE SCIENCE, 2012, 19(1): 70-74.
[6]	CHEN Xiao-rong, YANG Kong-song, FU Jun-ru, ZHU Chang-lan, PENG Xiao-song, HE Xiao-peng, HE Hao-hua . Identification and Genetic Analysis of Fertility Restoration Ability in Dongxiang Wild Rice (Oryza rufipogon) [J]. RICE SCIENCE, 2008, 15(1): 21-28 .