RICE SCIENCE

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Analysis of SSLP and Soluble Protein Contents in Leaves of Mutants Induced by High Pressure in Rice (Oryza sativa)

HE Xiu-ying1, XU Shi-ping2, LIAO Yao-ping1, MAO Xing-xue1, WENG Ke-nan2, CHEN Zhao-ming1, CHEN Yue-han1, XIAO Wan-sheng2   

  1. 1Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2 Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
  • Received:2003-10-08 Revised:1900-01-01 Online:2004-03-30 Published:2004-03-30

Abstract:

Rice variety Yuexiangzhan and its mutants induced by high pressure were studied using microsatellite markers and soluble protein content analyses. Eleven of the 88 microsatellite primer pairs showed evident polymorphisms repeatedly, and the polymorphic frequencies were 3.4-11.3% between the mutants and Yuexiangzhan. The polymorphic markers were randomly located on chromosomes. The more similar the plant types of the mutants like their original variety, the less polymorphic loci were detected. In addition, there was variation in the soluble protein contents among the leaves of mutants, and the contents were significantly lower than those of the original variety.

Key words: high pressure, mutant, microsatellite marker, soluble protein content