Introgression of QTLs Controlling Spikelet Fertility Maintains Membrane Integrity and Grain Yield in Improved White Ponni Derived Progenies Exposed to Heat Stress

doi:10.1016/j.rsci.2016.05.006

Abstract

Abstract:

To increase the thermotolerance of improved White Ponni (IWP), two quantitative trait loci (QTLs), qHTSF1.1 and qHTSF4.1, controlling spikelet fertility under high-temperature stress, were introgressed from Nagina 22 into IWP through marker-assisted breeding. The progenies were subjected to foreground selection of target QTLs using simple sequence repent markers RM431 and RM5757 linked to qHTSF1.1 and qHTSF4.1, respectively. At each generation, foreground selection with single target QTL or both QTLs was done together. The QTL-positive plants were forwarded to next generation by selfing. The F_2:3 progenies were subjected to phenotypic analyses under high-temperature stress at the flowering stage. Chlorophyll stability index, malondialdehyde content, grain yield, and yield-related components of the F_2:3 progenies were measured. The progenies IWP-295, IWP-277 and IWP-246 harboring both qHTSF1.1 and qHTSF4.1 showed higher fertility percentages under high-temperature stress at the flowering stage. These QTLs were responsible for maintaining membrane integrity and yield under elevated temperature conditions.

Key words: rice, QTL introgression, spikelet fertility, high temperature, membrane integrity, grain yield, malondialdehyde content

Vivitha P., Raveendran M., Vijayalakshmi D.. Introgression of QTLs Controlling Spikelet Fertility Maintains Membrane Integrity and Grain Yield in Improved White Ponni Derived Progenies Exposed to Heat Stress[J]. Rice Science, 2017, 24(1): 32-40.

Figures/Tables 7

References 32

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QTL	Marker	Plant number
qHTSF1.1	RM431	IWP-201, IWP-143, IWP-245, IWP-215, IWP-275
qHTSF4.1	RM5757	IWP-129, IWP-101, IWP-105, IWP-116, IWP-240
qHTSF1.1 and qHTSF4.1	RM431, RM5757	IWP-295, IWP-277, IWP-246, IWP-296, IWP-166, IWP-158, IWP-265, IWP-153, IWP-139, IWP-233
Absence of qHTSF1.1 and qHTSF4.1	RM431, RM5757	IWP-147, IWP-142, IWP-141, IWP-146, IWP-104

QTL	Marker	Plant number
qHTSF1.1	RM431	IWP-201, IWP-143, IWP-245, IWP-215, IWP-275
qHTSF4.1	RM5757	IWP-129, IWP-101, IWP-105, IWP-116, IWP-240
qHTSF1.1 and qHTSF4.1	RM431, RM5757	IWP-295, IWP-277, IWP-246, IWP-296, IWP-166, IWP-158, IWP-265, IWP-153, IWP-139, IWP-233
Absence of qHTSF1.1 and qHTSF4.1	RM431, RM5757	IWP-147, IWP-142, IWP-141, IWP-146, IWP-104

Date	Ambient chamber			Elevated chamber		Date	Ambient chamber			Elevated chamber
Date	T_max (ºC)	RH (%)		T_max (ºC)	RH (%)	Date	T_max (ºC)	RH (%)		T_max (ºC)	RH (%)
2015-03-01	34.0	86.3	36.2		84.1	2015-03-19	35.8	78.7	37.1		77.8
2015-03-02	34.2	85.6	36.2		83.7	2015-03-20	35.6	80.3	37.7		78.4
2015-03-03	34.0	85.9	36.1		83.0	2015-03-21	35.2	82.8	37.2		81.5
2015-03-04	33.0	90.3	35.2		88.1	2015-03-22	35.5	69.1	37.3		67.6
2015-03-05	34.4	85.1	36.7		84.2	2015-03-23	36.5	81.5	38.2		79.7
2015-03-06	34.0	83.2	36.5		82.5	2015-03-24	37.0	86.2	39.9		85.2
2015-03-07	34.0	89.6	36.1		88.7	2015-03-25	37.0	82.4	39.4		80.4
2015-03-08	33.7	83.7	35.8		81.0	2015-03-26	36.2	74.5	38.1		72.0
2015-03-09	33.2	80.0	35.4		78.5	2015-03-27	34.5	87.6	36.3		85.4
2015-03-10	33.0	81.4	36.5		80.4	2015-03-28	35.0	85.8	37.2		83.1
2015-03-11	33.8	79.4	36.0		77.2	2015-03-29	34.8	84.9	36.1		82.9
2015-03-12	33.2	83.5	35.7		81.9	2015-03-30	36.0	84.1	38.9		82.7
2015-03-13	32.5	79.1	35.3		77.8	2015-03-31	35.4	78.0	35.6		76.5
2015-03-14	33.6	78.9	35.2		77.5	2015-04-01	36.2	80.4	38.1		78.1
2015-03-15	33.5	82.8	35.5		80.1	2015-04-02	37.4	76.2	39.5		74.2
2015-03-16	31.7	85.6	33.6		84.6	2015-04-03	38.7	86.1	40.0		85.5
2015-03-17	33.4	83.5	36.2		81.7	2015-04-04	38.4	87.5	40.2		85.4
2015-03-18	34.8	77.4	36.0		75.6	2015-04-05	39.8	81.7	41.2		80.6

Date	Ambient chamber			Elevated chamber		Date	Ambient chamber			Elevated chamber
Date	T_max (ºC)	RH (%)		T_max (ºC)	RH (%)	Date	T_max (ºC)	RH (%)		T_max (ºC)	RH (%)
2015-03-01	34.0	86.3	36.2		84.1	2015-03-19	35.8	78.7	37.1		77.8
2015-03-02	34.2	85.6	36.2		83.7	2015-03-20	35.6	80.3	37.7		78.4
2015-03-03	34.0	85.9	36.1		83.0	2015-03-21	35.2	82.8	37.2		81.5
2015-03-04	33.0	90.3	35.2		88.1	2015-03-22	35.5	69.1	37.3		67.6
2015-03-05	34.4	85.1	36.7		84.2	2015-03-23	36.5	81.5	38.2		79.7
2015-03-06	34.0	83.2	36.5		82.5	2015-03-24	37.0	86.2	39.9		85.2
2015-03-07	34.0	89.6	36.1		88.7	2015-03-25	37.0	82.4	39.4		80.4
2015-03-08	33.7	83.7	35.8		81.0	2015-03-26	36.2	74.5	38.1		72.0
2015-03-09	33.2	80.0	35.4		78.5	2015-03-27	34.5	87.6	36.3		85.4
2015-03-10	33.0	81.4	36.5		80.4	2015-03-28	35.0	85.8	37.2		83.1
2015-03-11	33.8	79.4	36.0		77.2	2015-03-29	34.8	84.9	36.1		82.9
2015-03-12	33.2	83.5	35.7		81.9	2015-03-30	36.0	84.1	38.9		82.7
2015-03-13	32.5	79.1	35.3		77.8	2015-03-31	35.4	78.0	35.6		76.5
2015-03-14	33.6	78.9	35.2		77.5	2015-04-01	36.2	80.4	38.1		78.1
2015-03-15	33.5	82.8	35.5		80.1	2015-04-02	37.4	76.2	39.5		74.2
2015-03-16	31.7	85.6	33.6		84.6	2015-04-03	38.7	86.1	40.0		85.5
2015-03-17	33.4	83.5	36.2		81.7	2015-04-04	38.4	87.5	40.2		85.4
2015-03-18	34.8	77.4	36.0		75.6	2015-04-05	39.8	81.7	41.2		80.6

Progeny of IWP × N22	MDA content (µmol/g)		Percent increase over control (%)
Progeny of IWP × N22	Ambient temperature	High temperature	Percent increase over control (%)
Progeny only with allele of RM431
IWP-201	0.359 ± 0.004	0.690 ± 0.007	92.20
IWP-143	0.311 ± 0.003	0.500 ± 0.005	60.77
IWP-245	0.401 ± 0.004	0.608 ± 0.007	51.62
IWP-215	0.305 ± 0.004	0.502 ± 0.006	64.59
IWP-275	0.388 ± 0.004	0.676 ± 0.007	74.23
Progeny only with allele of RM5757
IWP-129	0.349 ± 0.004	0.655 ± 0.007	87.68
IWP-107	0.374 ± 0.004	0.599 ± 0.006	60.16
IWP-105	0.239 ± 0.003	0.550 ± 0.006	130.13
IWP-116	0.244 ± 0.003	0.642 ± 0.008	163.11
IWP-240	0.254 ± 0.003	0.542 ± 0.006	113.39
Progeny with both alleles of RM431 and RM5757
IWP-295	0.418 ± 0.005	0.572 ± 0.006	36.84
IWP-277	0.308 ± 0.003	0.456 ± 0.005	48.05
IWP-246	0.319 ± 0.004	0.412 ± 0.005	29.15
IWP-296	0.335 ± 0.004	0.509 ± 0.006	51.94
IWP-166	0.216 ± 0.002	0.447 ± 0.005	106.94
IWP-158	0.352 ± 0.004	0.578 ± 0.006	64.20
IWP-265	0.245 ± 0.003	0.472 ± 0.005	92.65
IWP-153	0.294 ± 0.003	0.604 ± 0.007	105.44
IWP-139	0.350 ± 0.004	0.667 ± 0.007	90.57
IWP-233	0.300 ± 0.003	0.502 ± 0.005	67.33
Progeny without alleles of RM431 and RM5757
IWP-147	0.350 ± 0.004	0.843 ± 0.009	140.86
IWP-142	0.365 ± 0.004	0.898 ± 0.010	146.03
IWP-141	0.498 ± 0.006	0.943 ± 0.010	89.36
IWP-146	0.314 ± 0.004	0.874 ± 0.010	178.34
IWP-104	0.341 ± 0.004	0.988 ± 0.011	189.74
Parent
IWP	0.326 ± 0.004	0.675 ± 0.007	107.06
N22	0.309 ± 0.003	0.423 ± 0.005	36.89
SE = 0.0082; CD = 0.0163 (P < 0.05).