Exogenous Peroxidase Mitigates Cadmium Toxicity, Enhances Rhizobial Population and Lowers Root Knot Formation in Rice Seedlings

doi:10.1016/j.rsci.2021.01.006

Abstract

Abstract:

Soil cadmium (Cd) causes toxicity and oxidative stress, alters biochemical processes and root knot formation in rice. Irrigation of exogenous peroxidase (POX) together with its co-substrate H₂O₂ (POX_Rice + H₂O₂), is likely to have protective effect upon the biochemical and nodular changes in rice grown in Cd-rich soil. Exposure to Cd concentration of 1.00 mg/L increased oxidative stress, loss of cell viability, electrolyte leakage and root knot formation, whereas it significantly lowered the chlorophyll level and rhizobium growth in rice. Irrigation of exogenous POX_Rice + H₂O₂ to Cd-stressed rice seedlings reversed the Cd-induced alterations in rice to levels similar in control (non-stressed) seedlings. Results provided strong evidence of exogenous POX_Rice + H₂O₂-mediated reversal and restoration of physiological and biochemical processes as well as increased resistance of rice seedlings to root knot formation. Irrigation with POX_Rice + H₂O₂ appeared to contribute towards bringing normoxic conditions in the otherwise hypoxic soil environment by enhancing the O₂ in pot-experiments due to reduced Cd uptake, enhanced mineral homeostasis of essential elements viz. P, Fe, Mo, Mg and Mn for maintenance of root architecture damaged by lipid peroxidation and reduction in oxidative stress by reducing Cd-induced reactive oxygen species generation. Therefore, the mitigation of Cd-toxicity in rice through this novel approach appeared to be a promising mode to limit Cd-uptake, modulate protective and tolerance mechanisms for sustainable rice yield in Cd-contaminated rice-croplands and prevent nematode attack in rice, however, more detailed studies are needed prior to large scale applications.

Key words: cadmium, peroxidase, rice, rhizobium, root knot, reactive oxygen species, antioxidant enzyme

Singh Priyanka, Pokharia Chitra, Shah Kavita. Exogenous Peroxidase Mitigates Cadmium Toxicity, Enhances Rhizobial Population and Lowers Root Knot Formation in Rice Seedlings[J]. Rice Science, 2021, 28(2): 166-177.

Figures/Tables 7

References 53

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Treatment	Cd	P	Fe	K	Mo	Mg	Mn
Control	1.8 ± 0.1 a	1 236.0 ± 7.8 a	3 118.0 ± 10.4 c	20 311.0 ± 35.5 b	6.3 ± 0.2 c	3 336.0 ± 31.0 b	163.0 ± 4.0 b
Cd	2.1 ± 0.1 b	1 271.0 ± 12.6 b	2 019.0 ± 10.2 a	21 037.0 ± 32.7 c	4.3 ± 0.2 a	3 163.0 ± 15.5 a	95.9 ± 4.2 a
Cd + POX_Rice + H₂O₂	2.0 ± 0.1 b	1 322.0 ± 12.5 c	2 513.0 ± 14.0 b	18 525.0 ± 167.6 a	5.2 ± 0.1 b	3 201.0 ± 13.0 a	161.0 ± 11.6 b

Treatment	Cd	P	Fe	K	Mo	Mg	Mn
Control	1.8 ± 0.1 a	1 236.0 ± 7.8 a	3 118.0 ± 10.4 c	20 311.0 ± 35.5 b	6.3 ± 0.2 c	3 336.0 ± 31.0 b	163.0 ± 4.0 b
Cd	2.1 ± 0.1 b	1 271.0 ± 12.6 b	2 019.0 ± 10.2 a	21 037.0 ± 32.7 c	4.3 ± 0.2 a	3 163.0 ± 15.5 a	95.9 ± 4.2 a
Cd + POX_Rice + H₂O₂	2.0 ± 0.1 b	1 322.0 ± 12.5 c	2 513.0 ± 14.0 b	18 525.0 ± 167.6 a	5.2 ± 0.1 b	3 201.0 ± 13.0 a	161.0 ± 11.6 b

Parameter	15-day-old seedling			30-day-old seedling
Parameter	Control	Cd	Cd + POX_Rice + H₂O₂	Control	Cd	Cd + POX_Rice + H₂O₂
Fresh weight (g)	0.22 ± 0.04 a	0.27 ± 0.01 b	0.24 ± 0.02 a	0.85 ± 0.05a	0.88 ± 0.07 a	0.85 ± 0.05 a
Dry weight (g)	0.04 ± 0.00 a	0.04 ± 0.00 a	0.04 ± 0.01 a	0.20 ± 0.02 a	0.20 ± 0.04 a	0.17 ± 0.01 a
Shoot length (cm)	14.70 ± 1.06 a	16.00 ± 1.07 b	15.00 ± 1.40 a	23.00 ± 2.20 b	20.60 ± 2.50 a	21.30 ± 3.10 a
Root length (cm)	4.00 ± 0.08 a	4.70 ± 0.80 a	7.00 ± 1.10 b	8.00 ± 1.40 a	7.80 ± 1.80 a	7.50 ± 1.20 a
EL [mol/(L∙cm)]	22.20 ± 3.02 a	37.50 ± 5.20 b	24.10 ± 2.70 a	24.60 ± 3.10 a	39.50 ± 5.30 c	33.20 ± 4.60 b
CC (mg/g)	0.27 ± 0.06 b	0.16 ± 0.04 a	0.22 ± 0.09 b	0.28 ± 0.05 b	0.19 ± 0.05 a	0.27 ± 0.02 b
Cell viability (%)	100.00	66.66 b	97.00 a	100.00	55.17 c	96.00 a
Parameter	75-day-old seedling			90-day-old seedling
Parameter	Control	Cd	Cd + POX_Rice + H₂O₂	Control	Cd	Cd + POX_Rice + H₂O₂
Fresh weight (g)	4.08 ± 0.33 b	2.88 ± 0.12 a	5.58 ± 0.64 c	2.90 ± 0.30 a	2.78 ± 0.20 a	5.87 ± 0.90 c
Dry weight (g)	0.47 ± 0.02 a	0.75 ± 0.05 b	1.42 ± 0.04 c	0.72 ± 0.06 a	0.64 ± 0.04 a	1.61 ± 0.07 b
Shoot length (cm)	35.00 ± 2.90 a	32.00 ± 4.20 a	39.00 ± 4.20 a	42.20 ± 6.10 b	37.40 ± 4.10 a	39.00 ± 5.20 a
Root length (cm)	10.00 ± 2.80 b	5.00 ± 1.10 a	11.00 ± 2.20 b	11.20 ± 2.70 b	11.00 ± 2.20 b	9.00 ± 2.10 a
EL [mol/(L∙cm)]	29.70 ± 2.50 b	42.40 ± 5.30 c	20.10 ± 3.70 a	38.30 ± 3.90 b	50.10 ± 5.70 c	33.10 ± 4.20 a
CC (mg/g)	0.29 ± 0.05 a	0.21 ± 0.09 a	0.26 ± 0.08 a	0.30 ± 0.01 b	0.22 ± 0.04 a	0.32 ± 0.03 b
Cell viability (%)	100.00	53.60 c	92.85 a	100.00	61.53 b	88.46 a

Parameter	15-day-old seedling			30-day-old seedling
Parameter	Control	Cd	Cd + POX_Rice + H₂O₂	Control	Cd	Cd + POX_Rice + H₂O₂
Fresh weight (g)	0.22 ± 0.04 a	0.27 ± 0.01 b	0.24 ± 0.02 a	0.85 ± 0.05a	0.88 ± 0.07 a	0.85 ± 0.05 a
Dry weight (g)	0.04 ± 0.00 a	0.04 ± 0.00 a	0.04 ± 0.01 a	0.20 ± 0.02 a	0.20 ± 0.04 a	0.17 ± 0.01 a
Shoot length (cm)	14.70 ± 1.06 a	16.00 ± 1.07 b	15.00 ± 1.40 a	23.00 ± 2.20 b	20.60 ± 2.50 a	21.30 ± 3.10 a
Root length (cm)	4.00 ± 0.08 a	4.70 ± 0.80 a	7.00 ± 1.10 b	8.00 ± 1.40 a	7.80 ± 1.80 a	7.50 ± 1.20 a
EL [mol/(L∙cm)]	22.20 ± 3.02 a	37.50 ± 5.20 b	24.10 ± 2.70 a	24.60 ± 3.10 a	39.50 ± 5.30 c	33.20 ± 4.60 b
CC (mg/g)	0.27 ± 0.06 b	0.16 ± 0.04 a	0.22 ± 0.09 b	0.28 ± 0.05 b	0.19 ± 0.05 a	0.27 ± 0.02 b
Cell viability (%)	100.00	66.66 b	97.00 a	100.00	55.17 c	96.00 a
Parameter	75-day-old seedling			90-day-old seedling
Parameter	Control	Cd	Cd + POX_Rice + H₂O₂	Control	Cd	Cd + POX_Rice + H₂O₂
Fresh weight (g)	4.08 ± 0.33 b	2.88 ± 0.12 a	5.58 ± 0.64 c	2.90 ± 0.30 a	2.78 ± 0.20 a	5.87 ± 0.90 c
Dry weight (g)	0.47 ± 0.02 a	0.75 ± 0.05 b	1.42 ± 0.04 c	0.72 ± 0.06 a	0.64 ± 0.04 a	1.61 ± 0.07 b
Shoot length (cm)	35.00 ± 2.90 a	32.00 ± 4.20 a	39.00 ± 4.20 a	42.20 ± 6.10 b	37.40 ± 4.10 a	39.00 ± 5.20 a
Root length (cm)	10.00 ± 2.80 b	5.00 ± 1.10 a	11.00 ± 2.20 b	11.20 ± 2.70 b	11.00 ± 2.20 b	9.00 ± 2.10 a
EL [mol/(L∙cm)]	29.70 ± 2.50 b	42.40 ± 5.30 c	20.10 ± 3.70 a	38.30 ± 3.90 b	50.10 ± 5.70 c	33.10 ± 4.20 a
CC (mg/g)	0.29 ± 0.05 a	0.21 ± 0.09 a	0.26 ± 0.08 a	0.30 ± 0.01 b	0.22 ± 0.04 a	0.32 ± 0.03 b
Cell viability (%)	100.00	53.60 c	92.85 a	100.00	61.53 b	88.46 a

Parameter	Tissue	15-day-old seedling			30-day-old seedling
Parameter	Tissue	Control	Cd	Cd + POX_Rice + H₂O₂	Control	Cd	Cd + POX_Rice + H₂O₂
MDA content (nmol/g)	Shoot	0.03 ± 0.01 a	0.04 ± 0.02 a	0.02 ± 0.02 a	0.03 ± 0.01 a	0.05 ± 0.02 a	0.03 ± 0.04 a
MDA content (nmol/g)	Root	0.02 ± 0.01 b	0.04 ± 0.01 c	0.01 ± 0.01 a	0.02 ± 0.01 ab	0.04 ± 0.03 b	0.02 ± 0.02 a
H₂O₂ content (µmol/g)	Shoot	4.10 ± 0.04 a	4.67 ± 0.06 b	4.02 ± 0.03 a	4.90 ± 0.06 a	5.12 ± 0.08 a	4.10 ± 0.05 a
H₂O₂ content (µmol/g)	Root	3.13 ± 0.91 a	4.12 ± 0.53 b	3.87 ± 0.45 b	3.30 ± 0.26 a	4.90 ± 0.59 b	3.80 ± 0.78 a
O₂·^‒ content (nmol/g)	Shoot	0.08 ± 0.01 a	0.17 ± 0.06 b	0.05 ± 0.08 a	0.08 ± 0.05 a	0.19 ± 0.03 a	0.06 ± 0.03 a
O₂·^‒ content (nmol/g)	Root	0.08 ± 0.01 b	0.15 ± 0.02 c	0.04 ± 0.01 a	0.08 ± 0.02 a	0.16 ± 0.05 a	0.07 ± 0.01 a
Parameter	Tissue	75-day-old seedling			90-day-old seedling
Parameter	Tissue	Control	Cd	Cd + POX_Rice + H₂O₂	Control	Cd	Cd + POX_Rice + H₂O₂
MDA content (nmol/g)	Shoot	0.04 ± 0.01 ab	0.06 ± 0.02 b	0.03 ± 0.01 a	0.04 ± 0.01 a	0.05 ± 0.02 a	0.04 ± 0.01 a
MDA content (nmol/g)	Root	0.02 ± 0.01 a	0.05 ± 0.00 b	0.03 ± 0.01 ab	0.03 ± 0.01 a	0.04 ± 0.01 a	0.03 ± 0.01 a
H₂O₂ content (µmol/g)	Shoot	5.00 ± 0.10 b	5.90 ± 0.30 c	4.60 ± 0.23 a	5.20 ± 0.30 a	5.67 ± 0.50 a	5.10 ± 0.60 a
H₂O₂ content (µmol/g)	Root	4.40 ± 0.47 ab	5.30 ± 0.39 b	4.10 ± 0.94 a	5.10 ± 0.94 a	5.40 ± 0.67 a	4.00 ± 0.75 a
O₂·^‒ content (nmol/g)	Shoot	0.09 ± 0.05 a	0.20 ± 0.02 a	0.08 ± 0.01 a	0.09 ± 0.07 a	0.23 ± 0.04 a	0.09 ± 0.01 a
O₂·^‒ content (nmol/g)	Root	0.09 ± 0.02 a	0.19 ± 0.05 a	0.08 ± 0.03 a	0.09 ± 0.03 a	0.21 ± 0.02 a	0.09 ± 0.02 a