NaCl Facilitates Cell Wall Phosphorus Reutilization in Abscisic Acid Dependent Manner in Phosphorus Deficient Rice Root

doi:10.1016/j.rsci.2023.01.006

Abstract

Abstract:

Phosphorus (P) starvation in rice facilitates the reutilization of root cell wall P by enhancing the pectin content. NaCl modulates pectin content, however, it is still unknown whether NaCl is also involved in the process of pectin regulated cell wall P remobilization in rice under P starved conditions. In this study, we found that 10 mmol/L NaCl increased the shoot and root biomasses under P deficiency to a remarkable extent, in company with the elevated shoot and root soluble P contents in rice. Further analysis indicated that exogenous NaCl enhanced the root cell wall P mobilization by increasing the pectin methylesterase activity and uronic acid content in pectin suggesting the involvement of NaCl in the process of cell wall P reutilization in P starved rice roots. Additionally, exogenous NaCl up-regulated the expression of P transporter OsPT6, which was induced by P deficiency, suggesting that NaCl also facilitated the P translocation prominently from root to shoot in P starved rice. Moreover, exogenous abscisic acid (ABA) can reverse the NaCl-mediated mitigation under P deficiency, indicating the involvement of ABA in the NaCl regulated root cell wall P reutilization. Taken together, our results demonstrated that NaCl can activate the reutilization of root cell wall P in P starved rice, which is dependent on the ABA accumulation pathway.

Key words: abscisic acid, cell wall, NaCl, phosphorus transporter, phosphorus deficiency, remobilization

Yang Xiaozheng, Liu Yusong, Huang Jing, Tao Ye, Wang Yifeng, Shen Renfang, Zhu Xiaofang. NaCl Facilitates Cell Wall Phosphorus Reutilization in Abscisic Acid Dependent Manner in Phosphorus Deficient Rice Root[J]. Rice Science, 2023, 30(2): 138-147.

Figures/Tables 8

Fig. 1. Effects of different concentrations of NaCl on growth performance of rice seedlings under P-sufficient (+P) and P-deficient (-P) conditions. A, Growth performances of rice seedlings under +P and -P conditions with different NaCl concentrations for 1, 4 and 7 d. Scale bars, 1 cm. B and C, Root (B) and shoot (C) biomasses of rice under +P or -P conditions with different NaCl concentrations. Data are Mean ± SD (n = 4). Asterisks indicate significant difference at P < 0.05 by the Student’s t test.

Fig. 2. Effects of 10 mmol/L NaCl on soluble P contents in root (A) and shoot (B) of rice under P-sufficient (+P) and P-deficient (-P) conditions for 1, 4 and 7 d. Data are Mean ± SD (n = 4). Different lowercase letters above the bars indicate significant differences at P < 0.05 by one-way analysis of variance.

Fig. 3. Effects of 10 mmol/L NaCl on transcription levels of OsSPX1 (A), OsSPX3 (B), OsSPX5 (C) and OsSPX6 (D) in rice roots under P-sufficient (+P) and P-deficient (-P) conditions for 7 d. OsActin was used as a reference gene. Data are Mean ± SD (n = 4). Different lowercase letters above the bars indicate significant differences at P < 0.05 by one-way analysis of variance.

Fig. 4. Effects of 10 mmol/L NaCl on P contents in cell wall (A and F) and pectin (B and G), uronic acid contents in pectin (C and H), pectin methylesterase (PME) activities (D and I) as well as pectin methyl esterification degrees (E and J) of rice roots and shoots under P-sufficient (+P) and P-deficient (-P) conditions for 1, 4 and 7 d. Data are Mean ± SD (n = 4). Different lowercase letters above the bars indicate significant differences at P < 0.05 by one-way analysis of variance.

Fig. 5. Effects of 10 mmol/L NaCl on rice xylem P content in P-sufficient (+P) and P-deficient (-P) solution. Data are Mean ± SD (n = 4). The significant differences at P < 0.05 are indicated by different lowercase letters above the bars.

Fig. 6. Effects of 10 mmol/L NaCl on transcripts of OsPT2 (A), OsPT6 (B) and OsPT8 (C) in P-sufficient (+P) and P-deficient (-P) solution in rice roots for 7 d. OsActin gene was used as a reference gene. Data are Mean ± SD (n = 4). Different lowercase letters above the bars indicate significant differences at P < 0.05 by one-way analysis of variance.

Fig. 7. Effects of NaCl on root abscisic acid (ABA) content (A), as well as ABA on root (B) and shoot (C) soluble P concentrations in P-sufficient (+P) and P-deficient (-P) solution with or without 10 mmol/L NaCl in rice. Data are Mean ± SD (n = 4). The significant differences at P < 0.05 are indicated by different lowercase letters above the bars.

Fig. 8. Proposed diagram of action pattern of NaCl in P starved rice. First, exogenous NaCl significantly decreased the accumulation of endogenous abscisic acid (ABA), which in turn increased the content of pectin and the activity of pectin methylesterase (PME) to reuse the cell wall fixed P. Second, exogenous NaCl facilitated the root to shoot translocation of P by increasing the transcription level of OsPT6. PM, Plasma membrane; Pi, Inorganic phosphorus.

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