Rice Science ›› 2025, Vol. 32 ›› Issue (4): 512-524.DOI: 10.1016/j.rsci.2025.02.003

• Research Papers • Previous Articles     Next Articles

Characteristic Analysis of Penta-Resistance Restorer Line for Hybrid Rice

Li Xinyan1,2, Weng Lüshui1, Xiao Youlun3, Li Jinjiang1, Deng Lihua1, Liu Qing1, Kang Weiwei1, Duan Yaping1, Yang Daji1, Xiao Guoying1,4()   

  1. 1Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
    3Hunan Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China
    4Yuelushan Laboratory, Changsha 410128, China
  • Received:2024-12-01 Accepted:2025-02-15 Online:2025-07-28 Published:2025-08-06
  • Contact: Xiao Guoying

Abstract:

Rice often suffers from pests, diseases, and weeds throughout its life cycle. The management of pests, diseases, and weeds with agrochemicals increases production costs and results in pesticide residues. Developing new germplasm and varieties with multiple resistances is the most economical, sustainable, and efficient way to solve these problems. In this study, marker-assisted backcrossing was used to pyramid multiple resistance genes, resulting in the development of a penta-resistance restorer line, B2A1920. This line was evaluated for its agronomic traits and resistance to diseases, pests, and herbicides. It was confirmed that B2A1920 contains the herbicide resistance gene Bar, the lepidopteran pest resistance gene Cry2Aa, the bacterial blight (BB) resistance gene Xa23, the blast resistance gene Pi2, and the brown planthopper (BPH) resistance genes Bph14 and Bph15. These genes confer resistance to glufosinate, lepidopteran pests, BB, blast, and BPH, respectively, while the line also exhibits elite agronomic traits. Its hybrids, Gui A × B2A1920, Quan 9311A × B2A1920, Yexiang A × B2A1920, Xianglong A × B2A1920, Tiantai A × B2A1920, and Shen 9A × B2A1920, likewise showed penta-resistance and superior agronomic performance. These results indicate the successful development of a cytoplasmic male sterile restorer line with high combining ability and penta-resistance.

Key words: hybrid rice, resistance, non-selective herbicide, rice blast, bacterial blight, lepidopteran pest, brown planthopper