Race Specificity of Major Rice Blast Resistance Genes to Magnaporthe grisea Isolates Collected from indica Rice in Guangdong, China

Abstract

Abstract: Race-specific resistance and field resistance of 30 rice blast resistance monogenic lines derived from different resources were evaluated. The spectra of resistance to 163 Magnaporthe grisea isolates collected from indica rice in Guangdong Province, China ranged from 0.6% to 89.6%. Most of the monogenic lines showed a narrow resistance spectrum and high susceptibility in rice blast area, whereas the lines with Pikh and Pi1(t) had the broad resistance spectra of 89.6% and 82.2% respectively, showing a high and stable blast resistance in fields. According to the cluster analysis of specific resistance to 163 blast isolates tested, the 30 monogenic lines were divided into 15 groups, and based on the principal factor analysis, nine kinds of race-specific resistance were identified. Pik, Piz5, Pi9 and Pish can be used as candidate resistance genes for rice breeding since their specific resistance differed from those of the backbone parents in Guangdong, China. Gene pyramiding of Pikh [or Pi1(t)], Pi9 (or Piz5) and Pish (or Pita2) will be effective to obtain broad-spectrum blast resistance in rice breeding program in Guangdong, China. The strategies for studying and application of rice blast resistance genes were discussed.

Key words: rice, race-specific resistance, rice blast, Magnaporthe grisea, field resistance, resistance gene

YANG Jian-yuan, CHEN Shen, ZENG Lie-xian, LI Yi-long, CHEN Zhen, LI Chuan-ying, ZHU Xiao-yuan. Race Specificity of Major Rice Blast Resistance Genes to Magnaporthe grisea Isolates Collected from indica Rice in Guangdong, China[J]. RICE SCIENCE, 2008, 15(4): 311-318 .

References

1 Ou S H. Breeding rice for resistance to blast: A critical view. In: IRRI. Proceedings of the Rice Blast Workshop. Manila: IRRI, 1979: 79–137.
2 Bonman J M, Mackill D J. Durable resistance to rice blast. Oryza, 1988, 25: 102–110.
3 Wang G L, Mackill D J, Bonman J M, McCouch S R, Champoux M C, Nelson R J. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistance rice cultivar. Genetics, 1994, 136(4): 1421–1434.
4 Wu S Z, Zhu X Y, Liu B, Yang Q Y, Zhang S H, Leung H. Genetic analysis and evaluation of durable resistance to blast in indica cultivar Sanhuangzhan 2. Sci Agric Sin, 2004, 37(4): 528–534. (in Chinese with English abstract)
5 Sallaud C, Lorieux M, Roumen E, Tharreau D, Berruyer R, Svestasrani P, Garsmeur O, Ghesquiere A, Notteghem J L. Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. Theor Appl Genet, 2003, 106(5): 794–803.
6 International Rice Genome Sequencing Project. The map- based sequence of the rice genome. Nature, 2005, 436(7052): 793–800.
7 Yu J, Wang J, Lin W, Li S G, Li H, Zhou J, Ni P X, Dong W, Hu S N, Zeng C Q, Zhang J G, Zhang Y, Li R Q, Xu Z Y, LiS T, Li X R, Zheng H K, Cong L J, Lin L, Yin J N, Geng J N, Li G Y, Shi J P, Liu J, Lu H, Li J, Wang J, Deng Y J, Ran L H, Shi X L, Wang X Y, Wu Q F, Li C F, Ren X Y, Wang J Q, Wang X L, Li D W, Liu D Y, Zhang X W, Ji Z D, Zhao W M, Sun Y Q, Zhang Z P, Bao J Y, Han Y J, Dong L L, Ji J, Chen P, Wu S M, Liu J S, Xiao Y, Bu D B, Tan J L, Yang L, Ye C, Zhang J F, Xu J Y, Zhou Y, Yu Y P, Zhang B, Zhuang S L, Wei H B, Liu B, Lei M, Yu H, Li Y Z, Xu H, Wei S L, He X M, Fang L J, Zhang Z J, Zhang Y Z, Huang X G, Su Z X, Tong W, Li J H, Tong Z Z. Li S L, Ye J, Wang L S, Fang L, Lei T T, Chen C, Chen H, Xu Z, Li H H, Huang H Y, Zhang F, Xu H Y, Li N, Zhao C F, Li S T, Dong L J, Huang Y Q, Li L, Xi Y, Qi Q H, Li W J, Zhang B, Hu W, Zhang Y L, Tian X J, Jiao Y Z, Liang X H, Jin J, Gao L, Zheng W M, Hao B L, Liu S Q, Wang W, Yuan L P, Cao M L, McDermott J, Samudrala R, Wang J, Wong G K, Yang H M. The genome of Oryza sativa: A history of duplication. PloS Biol, 2005, 3(2): 266–281.
8 Chen X W, Shang J J, Chen D X, Lei C L, Zou Y, Zhai W X, Liu G Z, Xu J C, Ling Z Z, Cao G, Ma B T, Wang Y P, Zhao X F, Li S G, Zhu L H. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J, 2006, 46(5): 794– 804.
9 Liu X Q, Lin F, Wang L, Pan Q H. The in silico map-based cloning of Pi36, a rice CC-NBS-LRR gene which confers race-specific resistance to the blast fungus. Genetics, 2007, 176: 2541–2549.
10 Qu S H, Liu G F, Zhou B, Bellizzi M, Zeng L Y, Dai L Y, Han B, Wang G L. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172(3): 1901–1914.
11 Sharma T R, Madhav M S, Singh B K, Shanker P. Jana T K, Dalal V, Pandit A, Singh A, Gaikwad K, Upreti1 H C, Singh N K. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea. Mol Genet Gen, 2005, 274(6): 569–578.
12 Zhou B, Qu S H, Liu G, Dolan M, Sakai H, Lu G D, Bellizzi M, Wang G L. The eight amino-acid differences within three leucine-rice repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant Microbe Interact, 2006, 19(11): 1216–1228.
13 Zhou J H, Wang J L, Jiang W R, Lei C L, Ling Z Z. Virulence genes diversity and geographic distribution of Pyricularia grisea in China. Acta Agron Sin, 2003, 29(5): 646–651. (in Chinese with English abstract)
14 Li J B, Li C Y, Chen Y, Lei C L, Ling Z Z. Evaluation of twenty-two blast resistance genes in Yunnan using monogenetic rice lines. Acta Phytophyl Sin, 2005, 32(2): 113– 119. (in Chinese with English abstract)
15 Liu W D, Ruan Z P, Zheng S Q, Zhao C J, Zhen W, Lu G P, Wang Z H. Resistance of rice major Pi-genes to the Magnaporthe grisea population in Fujian, China. Acta Phytopathol Sin, 2005, 35(6): 526–531. (in Chinese with English abstract)
16 Zhu X Y, Yang Q Y, Yang J Y, Lei C L, Wang J L, Ling Z Z. Differentiation ability of monogenic lines to Magnaporthe grisea in indica rice. Acta Phytopathol Sin, 2004, 34(4): 361– 368. (in Chinese with English abstract)
17 International Rice Research Institute. Standard Evaluation System for Rice. 4th edn. Manila: IRRI, 1996.
18 Jeon J S, Chen D, Yi G H, Wang G L, Ronald P C. Genetic and physical mapping of Pi5(t), a locus associated with broad spectrum resistance to rice blast. Mol Genet Gen, 2003, 269(2): 280–289.
19 Tsunematsu H, Yanoria J T Y, Ebron L A, Hayashi N, Ando I, Kato H, Imbe T, Khush G S. Development of monogenic lines of rice for blast resistance. Breeding Sci, 2000, 50(3): 229–234.
20 Ahn S W. International collaboration on breeding for resistance to rice blast. In: Zeigler R S. Rice Blast Disease. Wallingford, United Kingdom: CAB International, 1994: 137–153.
21 Campbell M A, Chen D, Ronald P C. Development of codominant amplified polymorphic sequence markers in rice that flank the Magnaporthe grisea resistance gene Pi7(t) in recombinant inbred line 29. Phytopathology, 2004, 94(3): 302–307.
22 Liu X Q, Wang L, Chen S, Lin F, Pan Q H. Genetic and physical mapping of Pi36(t), a novel rice blast resistance gene located on rice chromosome 8. Mol Genet Gen, 2005, 274(4): 394–401.

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.