Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice

Abstract

Abstract: From the progenies of a telotrisomic of chromosome 9 short arm of an indica rice variety, Zhongxian 3037, a phenotypical variant was selected. The variant plant had rolled leaves, dispersed plant type, as well as a low seed-setting rate. Cytological and molecular cytological investigations revealed two extra chromosomes, which were the shortest in somatic cells of the variant. Fluorescent in situ hybridization (FISH) analysis using a rice centromere specific DNA (RCS2) and a DNA sequence specific for chromosome 9 on premetaphase and pachytene chromosomes showed that these two chromosomes were the short arms of chromosome 9. That is to say, the variant was a telotetrasomic of chromosome 9. Among the 25 pachytene cells, the two telosomic chromosomes paired each other to form a bivalent and didn’t pair with other normal chromosome 9 as multivalents in 96% cells. However, the bivalent was easy to disassociate in advance.

Key words: rice, telotetrasomic, meiosis, fluorescent in situ hybridization, molecular cytology, chromosome beh

GONG Zhi-yun, GAO Qing-song, YU Heng-xiu, YI Chuan-deng, GU Ming-hong. Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice[J]. RICE SCIENCE, 2008, 15(3): 161-165 .

References

Hu C H. Studies on the development of twelve types of trisomic in rice with reference to genetic and breeding programme. J Agric Assoc, China: New Series, 1968, 63: 53–71.
2 Khush G S, Singh R J, Sur S C, Librojo A L. Primary trisomics of rice: Origin, morphology, cytology and use in linkage mapping. Genetics, 1984, 107: 141–163.
3 Cheng Z K, Yu H X, Pan X B. Cytological study on a telotrisomic in rice. J Jiangsu Agric Coll, 1997, 18(3): 43–45. (in Chinese with English abstract)
4 Cheng Z H, Li X, Yu H X. A new set of primary trisomics in indica rice, its breeding and cytological investigation. Acta Genet Sin, 1996, 23(5): 363–371. (in Chinese with English abstract)
5 Cheng Z K, Yan H H, Yu H X, Tang S C, Jiang J M, Gu M H, Zhu L H. Development and applications of a complete set of rice telotrisomics. Genetics, 2001, 157(1): 361–368.
6 Cheng Z K, Yan H H, Dang B Y, Hu Z M, Gu M H, Zhu L H. Microdissection and amplification of the chromosome arm 5S in a rice telo-tetrasomic. Chinese Sci Bull, 1998, 43 (7): 590– 594.
7 Wang Z X, Ideta O, Yoshimura A. Identification of extra chromosome of aneuploids and tetrasomics in rice and the use of these aneuploids in genome analysis. Breeding Sci, 1995, 45: 327–330.
8 Kurata N, Omura T. Karyotype analysis in rice: I. A new method for identifying all chromosome pairs. Jpn J Genet, 1978, 53: 251–255.
9 Wu H K. Note on preparing of pachytene chromosomes by double mordant. Sci Agric, 1967, 15: 40–44.
10 Jiang J, Gill B S, Wang G L, Ronald P C, Ward D C. Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artifical chromosomes. Proc Natl Acad Sci USA, 1995, 92: 4487– 4491.
11 Cheng Z K, Gu M H. Karyotype analysis for pachytene chromosome of indica, japonica rice and their hybrid. Acta Genet Sin, 1994, 21(5): 385–392. (in Chinese with English abstract)
12 Gong Z Y, Wu H K, Cheng Z K. Physical mapping of the 45S rDNA and 5S rDNA to rice prometaphase chromosome. Acta Genet Sin, 2002, 29(3): 241–244. (in Chinese with English abstract)
13 Cheng Z H, Buell C R, Wing R A, Jiang J M. Resolution of fluorescence in-situ hybridization mapping on rice mitotic prometaphase chromosomes, meiotic pachytene chromosomes and extended DNA fibers. Chrom Res, 2002, 10: 379–387.
14 Gao J H, Mao L P, Wang R Q. A study on the cytology and morphology of the tetrasomics in foxtail millet. Acta Agron Sin, 2000, 26(6): 801–804. (in Chinese with English abstract)
15 Hu B M, Zhang T Z, Pan J J. Studies of trisomic plants in upland cotton: II. Transmission of trisomic chromosome and production of tetrasomes. Acta Agron Sin, 1996, 22(3): 283–287. (in Chinese with English abstract)
16 Wang Z X, Iwata N. Aneuhaploids and tetrasomics in rice (Oryza sativa L.) derived from anther culture of trisomics. Genome, 1995, 38: 696–705.
17 Wang Z X, Iwata N. Production of n+1 plants and tetrasomics by means of anther culture of trisomics plants in rice (Oryza sativa L.). Theor Appl Genet, 1991, 83: 12– 16.
18 Yu H X, Cheng Z K, Li Xing, Gu M H. Isolation and cytological investigation on two tetrasomics in rice. Chinese J Rice Sci, 1999, 13(4): 193–196. (in Chinese with English abstract)
19 Cheng Z H, Buell C R, Wing R A, Gu M H, Jiang J M. Toward a cytological characterization of the rice genome. Genome Res, 2001, 11: 2133–2141.
20 Singh K, Multani D S, Khush G S. Secondary trisomics and telotrisomics of rice: Origin, characterization, and use in determining the orientation of chromosome map. Genetics, 1996, 143: 517–529.
21 de La Roche Saint-Andre C. Alternative ends: Telomeres and meiosis. Biochimie, 2008, 90: 181–189.

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.