Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

RICE SCIENCE ›› 2008, Vol. 15 ›› Issue (3): 179-185 .

• Research Paper • Previous Articles Next Articles

Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

LIU Wu-ge ^{1, #} ; JIN Su-juan ^{1, 2, #} ; ZHU Xiao-yuan ³; WANG Feng ¹; LI Jin-hua¹; LIU Zhen-rong ¹; LIAO Yi-long1; ZHU Man-shan¹; HUANG Hui-jun ¹; LIU Yi-bai ²

¹ Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; ² Agronomy College, Jiangxi Agricultural University, Nanchang 330045, China; ³ Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; #These authors contributed equally to this paper

Received:2008-04-07 Online:2008-09-28 Published:2008-09-28
Contact: WANG Feng
Supported by:
the grants from the High-Tech Research and Development Program of China (Grant No. 2001AA241011 and No. 2003AA212052), the Major Sci-Tech Program of Guangdong Province, China (Grant No. 2003A2010101 and No. 2006A2020201), and the Agricultural Scientific Program of Guangdong Province, China (Grant No. 2005B20101006).

Abstract

Abstract: The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines, RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery rate of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province, China were used to inoculate the improved lines and the original line GD-8S artificially. The resistance frequencies of the improved lines ranged from 76.47% to 100%, much higher than that of the original line GD-8S (9.09%). On the agronomic characters, there were no significant differences between the improved lines and GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance.

Key words: rice, blast resistance, thermo-sensitive genic male sterile line, molecular marker-assisted selectio

LIU Wu-ge, JIN Su-juan, ZHU Xiao-yuan, WANG Feng, LI Jin-hua, LIU Zhen-rong, LIAO Yi-long, ZHU Man-shan, HUANG Hui-jun, LIU Yi-bai. Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection[J]. RICE SCIENCE, 2008, 15(3): 179-185 .

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Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

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