Rice Science

RICE SCIENCE ›› 2008, Vol. 15 ›› Issue (3): 243-246 .

• Short Communication • Previous Articles     Next Articles

Construction of Double Right-Border Binary Vector Carrying Non-Host Gene Rxo1 Resistant to Bacterial Leaf Streak of Rice

XU Mei-rong1; XIA Zhi-hui2; ZHAI Wen-xue2; XU Jian-long1; ZHOU Yong-li1; LI Zhi-kang1   

  1. 1 National Key Facility for Crop Genetic Resources & Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Bejing 100081, China; 2 Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2008-02-14 Online:2008-09-28 Published:2008-09-28
  • Contact: ZHOU Yong-li

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Abstract: Rxo1 cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with Hpa I and Xma I. pMNDRBBin6 carrying the gene Rxo1 was acquired by ligation of blunt-end and cohesive end. The results of PCR, restriction enzyme analysis and sequencing indicated that the Rxo1 gene had been cloned into pMNDRBBin6. This double right-border binary vector, named as pMNDRBBin6-Rxo1, will play a role in breeding marker-free plants resistant to bacterial leaf streak of rice by genetic transformation.

Key words: Rxo1 gene, double right-border binary vector, rice, bacterial leaf streak, selectable marker-free pl