Changes in Activities of Three Enzymes Degrading Galactomannan During and Following Rice Seed Germination

Abstract

Abstract: To investigate the relationships among β-mannanase, β-mannosidase and α-galactosidase required for degrading galactomannan in cell wall during and following rice seed germination, the activities of the three enzymes and the effects of ABA and GA3 on them were surveyed. The activities of β-mannosidase and α-galactosidase presented in dry and pre-germinated rice seeds, and increased slowly during and following germination. However, the activity of β-mannanase was detected only after germination. GA3 could promote the activities of β-mannanase and α-galactosidase. ABA had little effect on the activities of β-mannosidase and α-galactosidase, but it could seriously inhibit the activity of β-mannanase.

Key words: germination, β-mannanase, β-mannosidase, α-galactosidase, enzyme activity, rice

REN Yan-fang, HE Jun-yu, WANG Xiao-feng. Changes in Activities of Three Enzymes Degrading Galactomannan During and Following Rice Seed Germination[J]. RICE SCIENCE, 2007, 14(4): 295-301 .

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