Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice

RICE SCIENCE ›› 2007, Vol. 14 ›› Issue (3): 235-238 .

• Short Communication • Previous Articles

Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice

YIN De-suo ^{1, 2}, SUN Xiao-qiong ³, LI Ke ^{1, 2}, WANG Shi-quan ^{1, 2}, DENG Qi-ming ^{1, 2}, LI Ping ^{1, 2}

¹ Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; ² Key Laboratory of Southwest Crop Genetic Resource and Improvement, Sichuan Agricultural University, Ministry of Education, Ya’an 625014, China;³ Sichuan Agricultural University High-Tech CO., LTD, Wenjiang 611130, China

Received:2007-03-05 Online:2007-09-28 Published:2007-09-28
Contact: LI Ping
Supported by:
the National High Technology Research and Development Program of China(Grant No. 2003AA212030), and the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)(Grant No. IRT0453).

Abstract

Abstract: A herbicide safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The transcript was increased 6 h after sprayed with the safener solution, and 4 days later, the expression still could be detected. The fragment was recycled from the poly-gel and sequenced, and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice database, and the amino acid sequence was 60-84% homologous to those of the Yippee genes in several eukaryotes. The fragment was extended to the whole long cDNA, and thus a primer pair was designed. RT-PCR analysis for the designed primer supported the induction result.

Key words: rice, chemical induction, cDNA, mRNA differential display, herbicide safener, inducible gene

YIN De-suo, SUN Xiao-qiong, LI Ke, WANG Shi-quan, DENG Qi-ming, LI Ping, . Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice[J]. RICE SCIENCE, 2007, 14(3): 235-238 .

References

1 Ren H W, Xiao F Z, Xing Z W. Chemically regulated expression systems and their applications in transgenic plants.Transg Res, 2003, 12: 529-540.
2 Gatz C. Chemical control of gene expression. Ann Rev Plant Physiol Plant Mol Biol, 1997, 48: 89-108.
3 Gatz C, Quail P H. Tn10-encoded tet repressor can regulate an operator-containing plant promoter. Proc Natl Acad Sci, USA,1988, 85: 1394-1397.
4 Gatz C, Frohberg C, Wendenburg R. Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV35S promoter in intact transgenic tobacco plants. Plant J, 1992, 2: 397-404.
5 Granger C L, Cyr R J. Characterization of the yeast copper-inducible promoter system in Arabidopsis thaliana. Plant Cell Rep, 2001, 20: 227-234.
6 Yves D, Alexis P, Gethin R, et al. The ethanol switch: A tool for tissue-specific gene induction during plant development. Plant J, 2003, 36: 918-930.
7 Aoyama T, Chua N H. A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J, 1997, 11(3): 605-612.
8 No D, Yao T P, Evans R M. Ecdysone-inducible gene expression in mammalian cells and transgene mice. Proc Natl Acad Sci, USA, 1996, 93: 3346-3351.
9 Zuo J, Niu Q W, Chua N H. An estrogen receptor-based transactivator XVE mediate highly inducible gene expression in transgenic plants. Plant J, 2000, 24: 265-273.
10 Hershey H P, Stoner T D. Isolation and characterization of cDNA clones for RNA species induced by substituted benzenesulfonamides in corn. Plant Mol Biol, 1991, 17: 679-690.
11 Hu T Z. The chemical-inducible expression system of plant genes. Mol Plant Breeding, 2003, 1(5): 731-736.
12 Gatz C, Lenk I. Promoters that respond to chemical inducers. Trends Plant Sci, 1998, 3: 352-358.
13 Hatzios K K. Herbicide safeners: Effective inducers of plant defense gene-enzyme systems. Phytoparasitica, 2003, 31: 3-7.
14 de Veylder L, van Montagu M, Inze D. Herbicide safenerinducible gene expression in Arabidopsis thaliana. Plant Cell Physiol, 1997, 38(5): 568-577.
15 Wang F G, Zhao J R, Guo J L, Sun S X. Review of research into establishing a DNA fingerprint database of new Chinese maize cultivars. Chinese Bull Bot, 2005, 22(1): 121-128. (in Chinese with English abstract)
16 Roxstrom-Lindquist K, Faye I. The Drosophila gene reveals a novel family of putative zinc binding proteins highly conserved among eukaryotes. Insect Mol Biol, 2001, 10: 77-86.

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Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice

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