Development and Substance Accumulation of Caryopsis in Transgenic Rice with Antisense Wx Gene

Abstract

Abstract: The development and substance accumulation of rice caryopsis were studied by using the transgenic japonica and indica rice with antisense Wx gene. The weight of caryopses in transgenic rice was lower than that in non-transgenic one, and the reduction in weight was significantly correlated to the reduction in amylose content. In caryopsis of transgenic rice, the number of endosperm cells was less than that in caryopsis of non-transgenic one, but the proliferation speed was considerably higher during the first six days after flowering (DAF). During the first nine DAF, the soluble sugar content of transgenic rice caryopsis was less than that of the non-transgenic one, but the situation was reverse after nine DAF. Moreover, the total starch content also declined with the decrease in amylose content of transgenic rice caryopsis, while the amylopectin content increased accordingly. Therefore, the composition of starch in caryopsis also changed, but it did not affect the accumulation of protein in transgenic rice caryopsis.

Key words: rice, transgene, caryopsis, development, substance accumulation, waxy gene

CHEN Gang, WANG Zhong, LIU Qiao-quan, XIONG Fei, GU Yun-jie, GU Guo-jun. Development and Substance Accumulation of Caryopsis in Transgenic Rice with Antisense Wx Gene[J]. RICE SCIENCE, 2006, 13(2): 106-112 .

References

1 Zhang Y H. Content of amylose in rice and its influential factors. Heilongjiang Agric Sci, 2002(3): 34-37. (in Chinese with English abstract)
2 Wang Z Y, Zheng F Q, Shen G Z, Zhang J L, Hong M M. The amylose content in rice endosperm is related to the post-transcriptional regulation of the waxy gene. Plant J, 1995, 7: 613-622.
3 Xu Y B, Shen Z T. Effects of waxy gene on yield components in rice. Acta Agron Sin, 1989, 15(3): 237-241. (in Chinese with English abstract)
4 He Z H, Shen Z T. Effects of rice wx gene on substance accumulation of grain. Sci Agric Sin, 1995, 28(4): 20-24. (in Chinese with English abstract)
5 Liu Q Q, Zhang J L, Wang Z Y, Hong M M, Gu M H. A highly efficient transformation system mediated by Agrobacterium tumefaciens in rice. Acta Phytophysiol Sin, 1998, 24(3): 259-271. (in Chinese with English abstract)
6 Chen X H, Liu Q Q, Wang Z Y, Wang X W, Cai X L, Zhang J L, Gu M H. Introduction of an antisense waxy gene into the main parent lines of indica hybrid rice. Chinese Sci Bull, 2002, 47(9): 684-688. (in Chinese with English abstract)
7 Liu Q Q, Wan Z Y, Chen X H, Cai X L, Zhang J L, Hong M M, Gu M H. Stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. Transgenic Res, 2003, 12(1): 71-82.
8 Liu Q Q, Wang X W, Chen X H, Wang Z Y, Tang S Z, Hong M M, Gu M H. Effect of dominant waxy character on kernel weight of transgenic rice with antisense Wx gene. Sci Agric Sin, 2002, 35(2): 117-122. (in Chinese with English abstract)
9 Zhang Z J, Zhu Q S, Wang Z Q. A method of counting endosperm cells in rice grain. J Jiangsu Agric Coll, 1996, 17(2): 7-11. (in Chinese with English abstract)
10 Zou Q. A Guide of Phytophysiological Experiment. Beijing: China Agricultural Press, 2000: 110-114. (in Chinese)
11 Gu X Y, Gu M H, Zhang Z G, Zuo W Z. A survey of effect of wx locus on kernel weight in rice. Hybrid Rice, 1995(6): 31-32,39. (in Chinese with English abstract)
12 Takeda K, Nakayama R, Saito K. Influence of waxy character on grain size. Japan J Breeding, 1975, 25(2): 87-92.
13 Zhang Z J, Zhu Q S, Wang Z Q, Yang J C. Proliferation and filling of endosperm cell in relation to the source-sink characteristics of rice varieties. Acta Agron Sin, 1998, 24(1): 21-27. (in Chinese with English abstract)
14 Gu Y J, Wang Z, Chen J, Zhao G Y. The structure and function of pericarp in rice. Acta Agron Sin, 2002, 28(4): 439-444. (in Chinese with English abstract)
15 Takeda K. Grain-filling process in waxy (glutinous) and non-waxy isogenic line of rice. Japan J Breeding, 1980, 30(4): 329-334.
16 Takeda K. Response of grain-filling to ripening conditions in waxy (glutinous) and non-waxy isogenic lines and their F1 hybrid of rice. Japan J Breeding, 1981, 31(1): 1-8.
17 Wang Z, Li W F, Gu Y J, Chen G, Shi H Y, Gao Y Z. Development of rice endosperm and the pathway of nutrients entering the ednosperm. Acta Agron Sin, 1995, 21(5): 520-527. (in Chinese with English abstract)
18 Zhang Z J, Wang Z Q, Zhu Q S. Proliferation of endosperm cell and its relation to the growth of grain in rice. Acta Agron Sin, 1998, 24(3): 257-264. (in Chinese with English abstract)
19 Boyer C D. Biochemical genetics of carbohydrate metabolism in source and sink tissue. In: Photoassimilate Distribution and Partitioning in Plant and Crops: source-sink relationships. New York: Marcel Kekker, 1996: 341-368.
20 He Z H, Hua J W, Shen Z T. Effect of rice wx gene on carbohydrate accumulation and amylase activity of germinating grain. Chinese J Rice Sci, 1994, 8(1): 21-26. (in Chinese with English abstract)
21 Liang K J, Lin W X, Li Y J, Yang D Y, Zhu Y, Liang Y Y, Guo Y C, He H Q. Developmental genetic analysis of soluble sugar content and genetic covariance between soluble sugar content and grain quality. Mol Plant Breeding, 2003, 1(2): 207-215. (in Chinese with English abstract)

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.