A Simplified Rice DNA Extraction Protocol for PCR Analysis

Abstract

Abstract: A simple protocol was established for DNA extraction using etiolated rice seedlings, whereby rice DNA was directly extracted in 0.5 mol/L NaOH solution in a single eppendorf tube. Results of comparative PCR analyses and electrophoresis showed that the DNA extracted using this method was as good and useful as that using standard CTAB method.

Key words: DNA extraction, rice, polymerase chain reaction, molecular marker, simple sequence repeats, transgene

CHEN Wen-yue , CUI Hai-rui , BAO Jin-song , ZHOU Xiang-sheng , SHU Qing-yao . A Simplified Rice DNA Extraction Protocol for PCR Analysis[J]. RICE SCIENCE, 2006, 13(1): 67-70 .

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