Effect of Phytosulfokine-α on Agrobacterium-Mediated Transformation in Rice

Abstract

Abstract: Phytosulfokine-α(PSK-α), a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK-α has some influence on agrobacterium-mediated transformation in rice, PSK-α at a series of concentrations was added into co-culture medium respectively. The results showed that PSK-α indeed affected the recovery of resistant calli and the transformation frequency of rice varieties Taipei 309 and Lijiangxintuanheigu. PSK-α at the concentration of 10 nmol/L could increase induction of resistant callus and efficiency of transformation, with a 11% and 4.9% top increase, respectively than the control. However, PSK-α at 200 nmol/L could inhibit the induction of the resistant calli. Further more, the effect of PSK-αon agrobacterium-mediated transformation is related with the concentration of 2, 4-D in selection medium. Higher induction rate of resistant calli was obtained from tissues treated with PSK-α plus 2 mg/L 2, 4-D.

Key words: phytosulfokine, rice, Agrobacterium-mediated transformation

CHEN De-xi, XU Zheng-jun, MA Bing-tian, LI Shi-gui. Effect of Phytosulfokine-α on Agrobacterium-Mediated Transformation in Rice[J]. RICE SCIENCE, 2005, 12(4): 255-260 .

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