Effects of Chilling Stress on Photosynthetic Rate and Chlorophyll Fluorescence Parameter in Seedlings of Two Rice Cultivars Differing in Cold Tolerance

Abstract

Abstract: A cold-tolerant cultivar, Xiangnuo 1, and a cold-sensitive cultivar, IR50, were used to study the influence of chilling on photosynthetic rate and chlorophyll fluorescence parameters in rice seedlings. The photosynthetic rates declined dramatically during chilling, and decreased by 48.7% and 67.5% in Xiangnuo 1 and IR50 seedlings, respectively, after being subjected to chilling treatment for two days. Chlorophyll fluorescence measurements showed that relatively higher q_P and q_NP in Xiangnuo 1 were maintained to dissipate the redundant excitation energy and protect the reaction centers from chill injury; accordingly, redundant excitation energy accumulated less in the reaction centers, and antenna systems were less injured by chilling in Xiangnuo 1. On the contrary, in IR50, q_P and q_NP declined rapidly while E_x increased, as the chilling persisted. This result indicated that the reaction centers and antenna systems in IR50 were damaged severely by chilling, which led to the lower photosynthetic rate.

Key words: chilling, rice, photosynthetic rate, chlorophyll fluorescence analysis

WANG Guo-li, GUO Zhen-fei. Effects of Chilling Stress on Photosynthetic Rate and Chlorophyll Fluorescence Parameter in Seedlings of Two Rice Cultivars Differing in Cold Tolerance[J]. RICE SCIENCE, 2005, 12(3): 187-191 .

References

1 Lyons J M. Chilling injury in plants. Ann Rev Plant Physiol, 1973, 24: 445–451.
2 Zeng N Y, He J X, Zhao W, Liang H G. Changes in components of pigments and proteins in rice photosynthetic membrane during chilling stress. Acta Bot Bor-Occ Sin, 2000, 20(1): 8–14. (in Chinese with English abstract)
3 Chen S N, Zou X J, Liang B. Electronmicroscope observation on membrane system of leaf cells of some varieties of rice seedlings with different cold- resistance. Plant Physiol Comm, 1997, 33(3): 191–194. (in Chinese)
4 Hetherington S E, He J, Smile R M. Photoinhibition at low temperature in chilling sensitive and resistant plants. Plant Physiol, 1989, 90: 1609–1615.
5 Weeden N F, Buchanan B B. Leaf cytosolic fructose-1,6-bisphosphatase—A potential targets in low temperature stress. Plant Physiol, 1983, 72: 259–261.
6 Li P, Liu H X, Wang Y R, Liang C Y, Guo Z Y. Effect of chilling on photosynthesis in flag leaves at primary heading stage of F1 hybrid rice cultivars and their parental lines (three lines). Acta Bot Sin, 1990, 32(6): 456–464. (in Chinese with English abstract)
7 Li P, Chen Y Z, Li X P. Effects of chilling stress temperature on photosynthesis and photosynthate transport in flag leaves of hybrid rice at milky stage. Acta Bot Sin, 1994, 37(7): 544–551. (in Chinese with English abstract)
8 Kaniuga Z, Zabek J, Sochanoeicz B. Photosynthetic apparatus in chilling-sensitive plants. Contribution of loosely bound managese to the mechanism of reversible in activation of Hill reaction activity following cold and dark storage and illumination of leaves. Planta, 1979, 144: 490–502.
9 Li M R, Liu H X, Wang Y R. Effects of low temperature and light on photosynthetic electron transmission activity in cucumber seedlings cotyledon. Acta Phytophysiol Sin, 1993, 19(1): 23–30. (in Chinese with English abstract)
10 He J X, Zeng Y, Yi J, Liang H G. Effects of low temperature on photochemical function and levels of thylakoid proteins of Chloroplasts in rice seedlings. Chinese J Rice Sci, 1999, 13(2): 99–103. (in Chinese with English abstract)
11 Kee S C, Martin B, Ort D R. The effects of chilling in the dark and in the light on photosynthesis of tomato: electron transfer reaction. Photosyn Res, 1986, 8: 41.
12 Xu C C, Jeon J A, Lee C H. Relative contribution of photochemical and non-photochemical routes to excitation energy dissipation in rice and barley illuminated at a chilling temperature. Physiol Plant, 1999, 107: 447–453.
13 Liu C L, Chen H P, Liu E E, Peng X X, Lu S Y, Guo Z F. Multiple tolerance of rice to abiotic stresses and its relationship with ABA accumulation. Acta Agron Sin, 2003, 29: 725–729. (in Chinese with English abstract)
14 Schreiber U, Schliwa U, Bilger W. Continuous recording of photochemical and nonphotochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosyn Res, 1986, 10: 51–62.
15 Adams Ⅲ W W, Demming-Adams B, Logan B A, Barker D H, Osmond C B. Rapid changes in xanthophyll cycle- dependent energy dissipation and photosystemⅡefficiency in two vines, Stephania japonica and Smilax australis. Growinng in the understory of an open Eucalyptus forest. Plant Cell Environ, 1999, 22:125–136.
16 Huang M Y, Guo Z F. Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity. Biol Plant, 2005, 49(1): 81–84.
17 Krause G H, Weis E. Chlorophyll fluorescence and photosynthesis: The basic. Ann Rev Plant Physiol Plant Mol Biol, 1991, 42: 313–349.
18 Xu D Q, Shen Y G. The limit factors of photosynthesis. In: Yu S W. Plant physiology and molecular biology. Beijing: Science Press, 1998. 262–277.
19 Li P, Li X P, Chen Y Z. Effect of chilling stress induced photoinhibition stress on chlorophyll fluorescence in flag leaves at heading stage in indica rice varieties with different cold tolerance. Chinese J Rice Sci, 2000, 14(2): 88–92. (in Chinese with English abstract)
20 Jung S, Steffen K L, Lee H J. Comparative photoinhibition of a high and low altitude ecotype of tomato to chilling stress under high and low light conditions. Plant Sci, 1998, 134: 69–77.
21 Mauro S, Dainsese P, Lannoye R, Bassi R. Cold-resistant and cold-sensitive maize lines differ in the phosphorylation of the photosystem II subunit, CP29. Plant Physiol, 1997, 115(1): 171–180.

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.