Gram-Positive Bacteria Associated with Rice in China and Their Antagonists Against the Pathogens of Sheath Blight and Bakanae Disease in Rice

Abstract

Abstract: It is necessary to understand the bacterial populations associated with rice so as to provide more information and natural resources for effective management of major diseases in rice. A survey on screening and identification of gram-positive bacteria was conducted during 1998–2004. Seven hundred and fifty-six rice samples were collected from Zhejiang, Jiangsu, Fujian and Yunnan Provinces, China. Over 1000bacterial isolates were isolated and tested for colony morphology, pathogenicity, and some characteristics of bacteriology including Gram staining, fluorescent pigment on Kings medium B and microscopic observation for endospore. Together with five standard reference strains, 74 representative gram-positive bacterial isolates were confirmed by Biolog and gas chromatographic analysis of fatty acid methyl esters. Five bacterial species of Bacillus and other three genera were identified and isolates from Bacillus sublitis and Bacillus megaterium, exhibited the most effective inhibition against the pathogens of sheath blight and bakanae disease of rice. A few isolates from Bacillus pumilus and Bacillus megaterium showed weak virulent on rice together with some virulent isolates, risk should be considered when isolates from these species were screened for biocontrol agents.

Key words: bacterial identification, bacterial population, antagonistic bacteria, sheath blight, bakanae disease, rice

LUO Jin-yan, XIE Guan-lin, LI Bin, LUO Yuan-chan, ZHAO Li-han, WANG Xiao , LIU Bo, LI Wen. Gram-Positive Bacteria Associated with Rice in China and Their Antagonists Against the Pathogens of Sheath Blight and Bakanae Disease in Rice[J]. RICE SCIENCE, 2005, 12(3): 213-218 .

References

1 Xie G L, Zhu G N, Ren X P. Diversity of pathogenic bacteria from rice seeds. Acta Phytopathol Sin, 2002, 32 (2): 114–120. (in Chinese with English abstract)
2 Chen Z Y, Xu Z G, Gao T D, Ni S K, Yan D F, Lu F, Liu Y F. Evaluation and utilization of antagonistic bacteria against rice sheath blight. Chinese J Rice Sci, 2000, 14(2): 98–102. (in Chinese with English abstract)
3 Li X M, Hu B S, Xu Z G, Mew T W. Threshold population sizes of Bacillus subtilis B5423-R to suppress the occurrence of rice sheath blight. Chinese J Rice Sci, 2003, 17(4): 360–364. (in Chinese with English abstract)
4 Li B, Xie G L, Soad A, Coosemans J. Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria. J Zhejiang Univ (Sci), 2005, 6B(6): 496–501.
5 Mew T W, Misra J K. A manual of rice seed health testing. Manila, Philippines: International Rice Research Institute, 1994. 29–46.
6 Xie G L, Mew T W. Diversity of nonpathogenic bacteria associated with seeds of single rice crop in Zhejiang Province. Biodiversity Sci, 2002, 10 (3): 311–317.
7 Xie G L, Mew T W. A leaf inoculation method for detection of Xanthomonas oryzae pv. oryzicola from rice seed. Plant Dis, 1998, 82(9): 1007–1011. (in Chinese with English abstract)
8 Schaad N W, Jones J B, Chun W. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. St. Paul, Minnesota: American Phytopathological Society Press, 2001. 250–260.
9 Vauterin L, Yang P, Swings J. Utilization of fatty acid esters for the differentiation of new Xanthomonas species. Int J Syst Bacteriol, 1995, 46: 298–304.
10 Cottyn B, Regalado E, Lanoot B, De Cleene M, Mew T W, Swings J. Bacterial populations associated with rice seed in the tropical environment. Phytopathology, 2001, 91(3): 282–292.
11 Zhao X H, Chen W L, Li D B. Selection of antagonistic bacteria to Xanthomonas oryzae pv. oryzae and interaction of phyllosphere microbes on rice leaf. Chinese J Rice Sci, 2000, 14(3): 161–164. (in Chinese with English abstract)
12 Amann R I, Ludwig W, Scleifer K H. Phylogenitic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev, 1995, 59: 143–169.

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.