A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice

Abstract

Abstract:

A new method of one-step PCR was devised for detecting the first nucleotide in the splice donor site of Wx intron 1. compared to the regular PCR-AccⅠmethod, the method can produce the same result for detecting +1 nucleotide of Wx intron 1. The reliability of the new method was confirmed with 30 rice varieties. The new technique is more convenient and cheaper than the regular PCR-AccⅠmethod, and could be widely deploded in rice molecular marker assistant selection.

Key words: rice, amylose content, polymerase chain reaction, research methodology

MAO Xing-xue, LIU Yan-zhuo, XIAO Xin, CHEN Jian-wei, LUO Wen-yong, LI Xiao-fang. A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice
[J]. RICE SCIENCE.

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