Effects of Grain Protein Content Selection on Protein Content and Key Enzyme Activity Involved in Nitrogen Metabolism in Progenies Derived from a Rice Cross

doi:10.1016/S1672-6308(08)60119-5

Abstract

Abstract: Two japonica rice parents (Tong 769 and Xixuan 1) and their progenies, significantly different in protein content of grains, were investigated to reveal the activities of proteinase in leaves and glutamine synthetase in grains, as well as the dynamic changes of soluble protein content in grains during rice grain filling. The results showed that the parents were very similar in protein content, however, advanced lines with different protein contents in grains and varied activities of proteinase and glutamine synthetase were acquired by consecutively directional selection of the grain protein content in their progenies. Moreover, the enzyme activity and the protein content in grains exceeded their parents during grain filling. The protein content in grains was positively related with the proteinase activity, and the soluble protein content was negatively related with the glutamine synthetase activity in grains to some extent.

Key words: rice, hybrid progeny, protein content, nitrogen metabolism, enzyme activity

HUANG Xing, JIN Zheng-xun, LI Xiao-guang, LIU Hong-liang, XU Mei-lan, ZHANG Feng-zhuan, ZHANG Zhong-chen. Effects of Grain Protein Content Selection on Protein Content and Key Enzyme Activity Involved in Nitrogen Metabolism in Progenies Derived from a Rice Cross[J]. RICE SCIENCE, 2010, 17(2): 156-160 .

References

1 Matsue Y. Differences in amylose content, amylographic characteristics and storage proteins of grain on primary and secondary rachis branches in rice. Jpn J Crop Sci, 1995, 64(3): 601–606. (in Japanese with English abstract)
2 Qian C R, Feng Y J, Yang J, Liu H Y, Jin Z X. Effects of protein content selection on cooking and eating properties of rice in early-generation of crosses. Chin J Rice Sci, 2007, 21(3): 323–326. (in Chinese with English abstract)
3 Lea D J, Robinson S A, Stewart G R. The enzymology and metabolism of glutamate, and asporagine. In: Miflin B J, Lea P J. The Biochemistry of Plants. Vol. 16. New York: Academic Press, 1990: 121–159.
4 Buchanan B B, Gruissem W, Jones R L. Biochemistry & Molecular Biology of Plants. Beijing: Science Press, 2002. (in Chinese)
5 Shen C G. Plant’s Aging Physiology and Molecular Biology. Beijing: China Agriculture Press, 2001. (in Chinese)
6 Lou C H, Wang X C. Physiology Base of Crop Yield Formation. Beijing: China Agriculture Press, 2001. (in Chinese)
7 Liu H Y, Yang J, Qian C R, Jin Z X. Effects of 6- benzylaminopurine on grain nitrogen metabolism during grain filling stage and cooking and eating quality of rice. Chin J Rice Sci, 2006, 20(6): 667–669. (in Chinese with English abstract)
8 Zhang Q, Ye M B, Xu L L, Zhang R X. Biochemical properties of proteases in aging wheat leaves. Plant Physiol Comm, 1997, 33(5): 336–338. (in Chinese with English abstract)
9 Luo L F, Zheng S X, Liao Y L, Nie J, Xie J, Xiang Y W. Effect of controlled release nitrogen fertilizer on protein quality of brown rice and key enzyme activity involved in nitrogen metabolism in hybrid rice. Chin J Rice Sci, 2007, 21(4): 403–410. (in Chinese with English abstract)
10 Wang L, Zhu J G, Zhu C W. Relationship between decrease in nitrogen content and activities of key enzymes related to nitrogen metabolism in rice leaves under elevated CO2 concentration. Chin J Rice Sci, 2008, 22(5): 499–506. (in Chinese with English abstract)
11 Xie Z J, Jiang D, Cao W X, Dai T B, Jing Q. Effects of post anthesis soil water status on the key regulatory enzymes of starch and protein accumulation in wheat grains. J Plant Physiol Mol Biol, 2003, 29(4): 309–316.
12 Zhu H M, Rong X M, Liu Q, Peng J W. Differences in contents of grain protein of different genotype rice varieties. J Hunan Agric Univ, 2001, 27(1): 13–16. (in Chinese with English abstract)
13 Tang X R, Guan C Y, Yu T Q. Study on material metabolism of yield and quality of different genotypic rice. J Hunan Agric Univ, 1999, 25(4): 279–282. (in Chinese with English abstract)
14 Tang X R, Yu T Q. Effects of density on activities of several enzymes in fodder hybrid rice and its relation to protein content and yield. Hybrid Rice, 2001, 16(6): 47–49. (in Chinese with English abstract)
15 Jin Z X, Qian C R, Yang J, Liu H Y, Piao Z Z. Changes in activities of glutamine synthetase during grain-filling and their relation to rice quality. Rice Sci, 2007, 14(3): 211–214.
16 Xiang Z F, Wang W Y, Ren W J, Wang X C. Effects of uniconazole on nitrogen metabolism and grain protein content of rice. Rice Sci, 2005, 12(2): 107–113.

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.