Genetic Analysis and Mapping of a Thermo-sensitive White Stripe-Leaf Mutant at Seedling Stage in Rice (Oryza sativa)

doi:10.1016/S1672-6308(09)60027-5

Abstract

Abstract: A thermo-sensitive white stripe-leaf mutant (tws) was selected from the M₂ progeny of a japonica variety, Jiahua 1, treated by 60Co γ-radiation. In comparison with the wild type parent, the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn normal green on the 5th leaf when grown at low temperatures (20°C and 24°C). Furthermore, the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermo-sensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws. In addition, by using SSR markers and an F₂ segregating population derived from the cross between the tws mutant and 9311, tws was mapped between the markers MM3907 and MM3928 with a physical distance of 86 kb on rice chromosome 4

Key words: rice, thermo-sensitivity, white stripe-leaf mutant, gene mapping, genetics

LI Chao, LIN Dong-zhi, DONG Yan-jun, YE Sheng-hai, ZHANG Xiao-ming. Genetic Analysis and Mapping of a Thermo-sensitive White Stripe-Leaf Mutant at Seedling Stage in Rice (Oryza sativa)[J]. RICE SCIENCE, 2010, 17(4): 276-281 .

References

Alberte R S, Hesketh J D, Hofstra G, Thornber J P, Naylor A W, Bernard R L, Brim C, Endrizzi J, Kohel R J. 1974. Composition and activity of the photosynthetic apparatus in temperature sensitive mutants of higher plants. Proc Natl Acad Sci USA, 71: 2414–2418.

Cui H R, Xia Y W, Gao M W. 2001. The effects of temperature on leaf color and chlorophyll biosynthesis of rice mutant W1. Acta Agric Nucl Sin, 15(5): 269–273. (in Chinese with English abstract)
Dong W Q, Dong Y J, Shi S J. 1995. Inheritance and fertility research of the thermo-sensitive albino seedling phenotype in double-functional genic male-sterile lines 7436S. China Seeds, (1): 19–21. (in Chinese)
Dong Y J, Shi S Y, Zhang H D, Chen S H, Sun Z X. 1994. Temperature response and inheritance of a thermo-sensitive seedling colour mutant in rice (Oryza sativa L.). J Genet & Breeding, 48: 423–426.

Dong Y J, Dong W Q, Zang X M, Shi S Y, Zhang H D. 1995. The genetic analysis of a thermo-sensitive seedling-colour mutant Fan5. Chin J Rice Sci, 9(4): 249–250. (in Chinese with English abstract)
Dong Y J, Dong W Q, Shi S Y, Jin Q S. 2001. Identification and genetic analysis of a thermo-sensitive seedling-colour mutant in rice (Oryza sativa L.). Breeding Sci, 51: 1–4.
Dwyer L M, Tollenaar M, Houwing L. 1991. A nondestructive method to monitor leaf greenness in corn. Can J Plant Sci, 71: 505–509.
Gálová E, Böhmová B, Sevcovicová A. 2000. Analysis of some barley chlorophyll mutants and their response to temperature stress. Photosynthetica, 38: 29–35.
Han B, Xue Y B, Li J Y, Deng X W, Zhang Q F. 2007. Rice functional genomics research in China. Phil Trans R Soc B, 362(1482): 1009–1021.
Hiroki S, Kensuke K, Ko N, Masahiro Y, Atsushi Y, Koh I. 2007. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 52: 512–527.
Kusumi K, Mizutani A, Nishimura M, Iba K. 1997. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 12(6): 1241–1250.Kurata N, Miyoshi K, Nonomura K, Ken I N, Yamazaki Y, Yukihiro I. 2005. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 46: 48–62.
Li N, Chu H W, Wen T Q, Zhang D B. 2007. Genetic analysis and mapping of the rice white midrib mutant Oswm. Acta Agric Shanghai, 23(1): 1–4. (in Chinese with English abstract)
Lincoln S E, Daly M J, Lander E S. 1993. Constructing genetics maps with MAPMAKER/EXP 3.0: A tutorial and reference manual. In: A Whitehead Institute for Biomedical Research Technical Report. 3rd edn. Cambridge MA: Whitehead Institute.
Liu W Z, Fu Y P, Hu G C, Si H M, Zhu L, Wu C, Sun Z X. 2007. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta, 226: 785–795.
Markwell J, Osterman J C. 1992. Occurrence of temperature- sensitive phenotypic plasticity in chlorophyll-deficient mutants of Arabidopsis thaliana. Plant Physiol, 98: 392–394.
Muray M G, Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 8(19): 4321–4325.
Pasini L, Bruschini S, Bertoli A, Mazza R, Fracheboud Y, Marocco A. 2005. Photosynthetic performance of cold-sensitive mutants of maize at low temperature. Physiol Plant, 124: 362–370.

Shu Q Y, Liu G F, Xia Y W. 1996. The research of leaf color thermo- sensitive mutant. Acta Agric Nucl Sin, 10(1): 6–10. (in Chinese with English abstract)
Soo C Y, Sung H C, Hiroki S, Li J J, Kensuke K, Hee J K, Koh I, Nam C P. 2009. Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 150: 388–401.
Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, Iba K. 2004. The virescent 2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 45(8): 985–996.
Watanabe S, Hatanaka Y, Inada K. 1980. Development of a digital chlorophyll meter: I. Structure and performance. Jpn J Crop Sci, 49(Special issue): 89–90.

Xia J C, Wang Y P, Ma B T, Yin Z Q, Hao M, Kong D W, Li S G. 2006. Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.). J Genet Genom, 33(12): 1112–1119.

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