RICE SCIENCE ›› 2010, Vol. 17 ›› Issue (1): 1-9 .DOI: 10.1016/S1672-6308(08)60098-0

• Research Paper •     Next Articles

Cloning and Expression Analysis of an AP2/ERF Gene and Its Responses to Phytohormones and Abiotic Stresses in Rice

MA Hao-li1, ZHOU Han-lin1, 2, ZHANG Huai-yu2, ZHAO Jie1   

  1. 1Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China; 2College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2009-03-16 Revised:1900-01-01 Online:2010-03-28 Published:2010-03-28
  • Contact: ZHAO Jie
  • Supported by:

    the National Natural Science Foundation of China (Grant Nos. 30770132 and 30570103).

Abstract: Ethylene response factors (ERFs) play important roles in response to plant biotic and abiotic stresses. In this study, a gene encoding a putative AP2/ERF domain-containing protein was isolated by screening a SSH cDNA library from rice and designated as Oryza sativa AP2/ERF-like protein (OsAP2LP) gene. OsAP2LP is 1491 bp in length, interrupted by seven introns, and encodes a putative protein of 348 amino acids. Temporal and spatial expression analysis showed that the OsAP2LP gene was preferentially expressed in roots, panicles, mature embryos and seeds in rice. Real-time quantitative PCR analysis indicated that the expression levels of the OsAP2LP gene were increased under the treatments of drought and gibberellin but decreased under the treatments of low temperature, salt, abscisic acid (ABA) and zeatin. Taken together, these results suggest that OsAP2LP might be involved in stress responses, and probably plays roles as a transcription regulator when plants response to cold, salt and drought stresses through ABA and gibberellin pathways.

Key words: AP2/ERF gene, gene cloning, transcription factor, phytohormone, abiotic stress, rice (Oryza sativa)