New Temperature Sensitive Genic Male Sterile Lines with Better Outcrossing Ability for Production of Two-Line Hybrid Rice

doi:10.1016/S1672-6308(14)60273-0

Abstract

Abstract:

An investigation was carried out with three newly developed temperature sensitive genic male sterile (TGMS) lines for their floral traits, seed production potential and outcrossing ability in ten cross combinations. In the TGMS lines, fertile pollens had an average diameter of 0.89 mm while the sterile pollens was with 0.02 mm diameter.TS-29-150GY produced the biggest fertile pollens with 0.92 mm and other two lines produced relatively smaller pollens with 0.91 and 0.85 mm. Pollen fertility during the fertility reversion period was an average of 60.7%. TS-29-150GY had the maximum of 66.9% spikelet fertility whereas other two lines (TNAU18S and TNAU60S) had relatively lower spikelet fertility of 27.8% and 26.7%, respectively. Average of 17.00 g of seed yield was obtained in the TGMS lines during the fertility reversion period. TS-29-150GY had the highest value of 21.20 g of seed yield while TNAU18S and TNAU60S produced 16.6 g and 13.2 g of seed yield, respectively. The low seed production ability of these three TGMS lines was attributed only to the environmental conditions prevailing during the period. All three TGMS lines had considerable outcrossing potential of 41.2%, 24.6% and 25.0%, respectively. The cross combinations viz. TNAU18S/IET21508 (36 g/plant), TNAU18S/IET21044 (13 g/plant), TNAU18S/IET21009 (26.5 g/plant), TNAU60S/CB-09-106 (26.2 g/plant), TNAU60S/IET21009 (14 g/plant) and TS29-150-GY/DRR 3306 (39.2 g/plant) showed perfect synchronization with acceptable hybrid seed yield, indicating suitability of TGMS system under Indian condition. Based on the outcrossing related traits viz. panicle exertion, angle of glume opening, stigma length and pollen size, TNAU18S was identified as the best, followed by TS-29-150GY.

Key words: temperature sensitive genic male sterile line, outcrossing, floral trait, hybrid rice

J. Arasakesary S., Manonmani S., Pushpam R., Robin S.. New Temperature Sensitive Genic Male Sterile Lines with Better Outcrossing Ability for Production of Two-Line Hybrid Rice[J]. Rice Science, 2015, 22(1): 49-52.

Figures/Tables 2

References 13

1	Abeyesekera S W, De D S, Abeysiriwardana Z,. Dehideniya E M G J P K.2003. Characteristics associated with outcrossing rate of cytoplasmic male sterile (CMS) lines in rice under local conditions. Ann Sri Lanka Dept Agric, 5: 1-6.
2	Ali J.1993. Studies on temperature sensitive genic male sterility and chemical induced sterility towards development of two-line hybrids in rice (Oryza sativa L.) [PhD Thesis]. New Delhi, India: Indian Agricultural Research Institute.
3	Jones J W.1926. Hybrid vigour in rice. J Am Soc Agron, 18(3): 424‒428.
4	Kalaiyarasi R, Vaidyanathan P.2002. Cytological differentiation of TGMS gene expression in rice indica/japonica derivatives. In: Proceedings of the 4th International Symposium on Hybrid Rice, 14‒17 May, 2002. Hanoi, Vietnam. Los Banos, Philippines: International Rice Research Institute: 357‒371.
5	Karpagam V.2011. Development of two-line hybrids through exploitation of thermo-sensitive genic male sterility system in rice (Oryza saliva L) [MS Thesis]. Coimbatore, India: Tamil Nadu Agricultural University.
6	Lopez M T, Virmani S S.2000. Development of TGMS lines for developing two-line rice hybrids for the tropics. Euphytica, 114(2): 211‒215.
7	Ramaiah K.1933. Inheritance of flowering duration in rice. Ind J Agric Sci, 3(3): 377‒410.
8	Siddiq E A, Ali J.1999. Innovative male sterility systems for exploitation of hybrid vigour in crop plants: I. Environment sensitive genic male sterility system. PINSA B, 65(6): 331‒350.
9	Virmani S S, Viraktamath B C, Lopez M T.1997. Nucleus and breeder seed production of thermosensitive genic male sterile line. Int Rice Res Notes, 22: 26‒27.
10	Virmani S S, Sun Z X, Mou T M, Jauhr Ali A, Mao C X.2003. Two-Line Hybrid Rice Breeding Manual. Manila, Philippines: International Rice Research Institute.
11	Yuan L P.1977. The execution and theory of developing hybrid rice. Chin Agri Sci, 1(1): 27‒31. (in Chinese)
12	Yuan L P.1990. Progress of two-line system hybrid rice breeding. Sci Agr Sin, 3: 1‒6. (in Chinese with English abstract)
13	Yuan L P.1997. Exploiting crop heterosis by two-line system hybrids: Current status and future prospects. In: Proceedings of International Symposium on Two-Line System of Heterosis Breeding in Crops. Sep. 6‒8, 1997. Changsha, China: China National Hybrid Rice Research and Development Centre: 215‒220.

TGMS line	PE (%)	PF (%)	SO (%)	SPF (%)	AL (mm)	AB (mm)	FPD (mm)	SPD (mm)	SL (mm)	AGO (°)	SY (g)
TNAU18S	92.8	60	20.2	26.6	1.84	0.37	0.91	0.03	2.01	23	16.6
TNAU60S	91.8	61	15.5	27.8	2.21	0.41	0.85	0.01	1.75	18.5	13.2
TS-29-150GY	90.6	61	15.3	66.9	1.92	0.38	0.92	0.01	1.85	20.2	21.2
Mean	91.7	60.7	17	40.4	1.99	0.39	0.89	0.02	1.87	20.6	17

TGMS line	PE (%)	PF (%)	SO (%)	SPF (%)	AL (mm)	AB (mm)	FPD (mm)	SPD (mm)	SL (mm)	AGO (°)	SY (g)
TNAU18S	92.8	60	20.2	26.6	1.84	0.37	0.91	0.03	2.01	23	16.6
TNAU60S	91.8	61	15.5	27.8	2.21	0.41	0.85	0.01	1.75	18.5	13.2
TS-29-150GY	90.6	61	15.3	66.9	1.92	0.38	0.92	0.01	1.85	20.2	21.2
Mean	91.7	60.7	17	40.4	1.99	0.39	0.89	0.02	1.87	20.6	17

Cross combination	Plant height (cm)			Duration of flowering synchrony (d)	Outcrossing percentage (%)	Seed yield (g)
Cross combination	Female parent	Male parent	Difference in height	Duration of flowering synchrony (d)	Outcrossing percentage (%)	Seed yield (g)
TNAU18S/IET21009	64.2	115.4	51.2	15	27.5	26.5
TNAU18S/IET21508	58.4	86.2	27.8	12	39.3	36
TNAU18S/IET21044	60.8	114.8	54	14	12.5	13
TNAU18S/IET20921	62.4	98.4	36	18	8.3	9.6
TNAU60S/IET21493	63.4	87.4	24	10	8.9	12
TNAU60S/IET21513	64	115.4	51.4	15	17.6	18.5
TNAU60S/CB-09-106	74.4	96.4	22	16	11.3	26.2
TS-29-150GY/CB-09-106	66.8	90	23.2	6	4.7	1.9
TS-29-150GY/DRR3306	62.2	85.4	23.2	11	24.6	39.2
TNAU60S/IET21009	65.6	88.4	22.8	12	17.2	14
Mean	64.2	97.8	33.6	12.9	17.2	19.69

Cross combination	Plant height (cm)			Duration of flowering synchrony (d)	Outcrossing percentage (%)	Seed yield (g)
Cross combination	Female parent	Male parent	Difference in height	Duration of flowering synchrony (d)	Outcrossing percentage (%)	Seed yield (g)
TNAU18S/IET21009	64.2	115.4	51.2	15	27.5	26.5
TNAU18S/IET21508	58.4	86.2	27.8	12	39.3	36
TNAU18S/IET21044	60.8	114.8	54	14	12.5	13
TNAU18S/IET20921	62.4	98.4	36	18	8.3	9.6
TNAU60S/IET21493	63.4	87.4	24	10	8.9	12
TNAU60S/IET21513	64	115.4	51.4	15	17.6	18.5
TNAU60S/CB-09-106	74.4	96.4	22	16	11.3	26.2
TS-29-150GY/CB-09-106	66.8	90	23.2	6	4.7	1.9
TS-29-150GY/DRR3306	62.2	85.4	23.2	11	24.6	39.2
TNAU60S/IET21009	65.6	88.4	22.8	12	17.2	14
Mean	64.2	97.8	33.6	12.9	17.2	19.69

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