Identification of Rice Accessions Associated with K+/Na+ Ratio and Salt Tolerance Based on Physiological and Molecular Responses

doi:10.1016/j.rsci.2017.10.002

Abstract

Abstract:

The key for rice plant survival under NaCl salt stress is maintaining a high K⁺/Na⁺ ratio in its cells. Selection for salt tolerance rice genotypes based on phenotypic performance alone will delay in progress in breeding. Use of molecular markers in tandem with physiological studies will help in better identification of salt tolerant rice accessions. Eight rice accessions along with the check Dongjin were screened using 1/2 Yoshida solution with 50 mmol/L NaCl at the seedling stage. The accessions IT001158, IT246674, IT260533 and IT291341 were classified as salt tolerant based on their K⁺/Na⁺ ratios. Seventeen SSR markers reported to be associated with K⁺/Na⁺ ratio were used to screen the accessions. Five SSR markers (RM8053, RM345, RM318, RM253 and RM7075) could differentiate accessions classified based on their K⁺/Na⁺ ratios. Banding pattern of the accessions was scored compared to the banding pattern of Dongjin. The study differentiated accessions based on their association of K⁺/Na⁺ ratio with molecular markers which are very reliable. These markers can play a significant role in screening large set of rice germplasms for salt tolerance and also help in identification of high-yielding varieties with better salt tolerance. The salt tolerant accessions can be taken forward into developing better varieties by conventional breeding and exploring genes for salt tolerance.

Key words: rice, salinity, K⁺/Na⁺ ratio, simple sequence repeat, salt tolerance

Naga Bheema Lingeswara Reddy Inja, Kim Sung-Mi, Kim Beom-Ki, Yoon In-Sun, Kwon Taek-Ryoun. Identification of Rice Accessions Associated with K⁺/Na⁺ Ratio and Salt Tolerance Based on Physiological and Molecular Responses[J]. Rice Science, 2017, 24(6): 360-364.

Figures/Tables 6

References 32

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Accession	Origin	Type	Sub-population
IT001158	India	Introduced cultivar	Indica
IT246674	Bangladesh	Introduced cultivar	Not available
IT260533	India	Not available	Indica
IT291341	Iran	Introduced cultivar	Indica
IT168699	South Korea	Weedy	Japonica
IT175218	South Korea	Weedy	Japonica
IT219993	China	Not available	Not available
IT227934	South Korea	Landrace	Japonica
Dongjin	South Korea	Breeding line	Japonica

Accession	Origin	Type	Sub-population
IT001158	India	Introduced cultivar	Indica
IT246674	Bangladesh	Introduced cultivar	Not available
IT260533	India	Not available	Indica
IT291341	Iran	Introduced cultivar	Indica
IT168699	South Korea	Weedy	Japonica
IT175218	South Korea	Weedy	Japonica
IT219993	China	Not available	Not available
IT227934	South Korea	Landrace	Japonica
Dongjin	South Korea	Breeding line	Japonica

Name	Forward primer (5′-3′)	Reverse primer (5′-3′)	AT (^oC)	Polymorphic	Reference
RM5	TGCAACTTCTAGCTGCTCGA	GCATCCGATCTTGATGGG	56	Monomorphic	Koyama et al, 2001
RM261	CTACTTCTCCCCTTGTGTCG	TGTACCATCGCCAAATCTCC	56	Monomorphic	Koyama et al, 2001
RM136	GAGAGCTCAGCTGCTGCCTCTAGC	GAGGAGCGCCACGGTGTACGCC	64	Monomorphic	Yao et al, 2005
RM13	TCCAACATGGCAAGAGAGAG	GGTGGCATTCGATTCCAG	56	Monomorphic	Yao et al, 2005
RM345	ATTGGTAGCTCAATGCAAGC	GTGCAACAACCCCACATG	55	Polymorphic	Yao et al, 2005
RM176	CGGCTCCCGCTACGACGTCTCC	AGCGATGCGCTGGAAGAGGTGC	64	Monomorphic	Yao et al, 2005
RM262	CATTCCGTCTCGGCTCAACT	CAGAGCAAGGTGGCTTGC	58	Monomorphic	Yao et al, 2005
RM318	GTACGGAAAACATGGTAGGAAG	TCGAGGGAAGGATCTGGTC	55	Polymorphic	Yao et al, 2005
RM104	GGAAGAGGAGAGAAAGATGTGTGTCG	TCAACAGACACACCGCCACCGC	64	Not reproducible	Yao et al, 2005
RM336	CTTACAGAGAAACGGCATCG	GCTGGTTTGTTTCAGGTTCG	55	Not reproducible	Bhowmik et al, 2009
RM7075	TATGGACTGGAGCAAACCTC	GGCACAGCACCAATGTCTC	55	Polymorphic	Bhowmik et al, 2009
RM253	TCCTTCAAGAGTGCAAAACC	GCATTGTCATGTCGAAGCC	55	Polymorphic	Bhowmik et al, 2009
RM8053	AGACATTGCCGATGATAGG	AAGTACCCCACCGAATAGAG	55	Polymorphic	Senguttuvel et al, 2010
RM9	GGTGCCATTGTCGTCCTC	ACGGCCCTCATCACCTTC	58	Not reproducible	Senguttuvel et al, 2010
RM212	CCACTTTCAGCTACTACCAG	CACCCATTTGTCTCTCATTATG	55	Monomorphic	Senguttuvel et al, 2010
RM23	CATTGGAGTGGAGGCTGG	GTCAGGCTTCTGCCATTCTC	55	Monomorphic	Senguttuvel et al, 2010
RM493	TAGCTCCAACAGGATCGACC	GTACGTAAACGCGGAAGGTG	58	Monomorphic	Senguttuvel et al, 2010

Name	Forward primer (5′-3′)	Reverse primer (5′-3′)	AT (^oC)	Polymorphic	Reference
RM5	TGCAACTTCTAGCTGCTCGA	GCATCCGATCTTGATGGG	56	Monomorphic	Koyama et al, 2001
RM261	CTACTTCTCCCCTTGTGTCG	TGTACCATCGCCAAATCTCC	56	Monomorphic	Koyama et al, 2001
RM136	GAGAGCTCAGCTGCTGCCTCTAGC	GAGGAGCGCCACGGTGTACGCC	64	Monomorphic	Yao et al, 2005
RM13	TCCAACATGGCAAGAGAGAG	GGTGGCATTCGATTCCAG	56	Monomorphic	Yao et al, 2005
RM345	ATTGGTAGCTCAATGCAAGC	GTGCAACAACCCCACATG	55	Polymorphic	Yao et al, 2005
RM176	CGGCTCCCGCTACGACGTCTCC	AGCGATGCGCTGGAAGAGGTGC	64	Monomorphic	Yao et al, 2005
RM262	CATTCCGTCTCGGCTCAACT	CAGAGCAAGGTGGCTTGC	58	Monomorphic	Yao et al, 2005
RM318	GTACGGAAAACATGGTAGGAAG	TCGAGGGAAGGATCTGGTC	55	Polymorphic	Yao et al, 2005
RM104	GGAAGAGGAGAGAAAGATGTGTGTCG	TCAACAGACACACCGCCACCGC	64	Not reproducible	Yao et al, 2005
RM336	CTTACAGAGAAACGGCATCG	GCTGGTTTGTTTCAGGTTCG	55	Not reproducible	Bhowmik et al, 2009
RM7075	TATGGACTGGAGCAAACCTC	GGCACAGCACCAATGTCTC	55	Polymorphic	Bhowmik et al, 2009
RM253	TCCTTCAAGAGTGCAAAACC	GCATTGTCATGTCGAAGCC	55	Polymorphic	Bhowmik et al, 2009
RM8053	AGACATTGCCGATGATAGG	AAGTACCCCACCGAATAGAG	55	Polymorphic	Senguttuvel et al, 2010
RM9	GGTGCCATTGTCGTCCTC	ACGGCCCTCATCACCTTC	58	Not reproducible	Senguttuvel et al, 2010
RM212	CCACTTTCAGCTACTACCAG	CACCCATTTGTCTCTCATTATG	55	Monomorphic	Senguttuvel et al, 2010
RM23	CATTGGAGTGGAGGCTGG	GTCAGGCTTCTGCCATTCTC	55	Monomorphic	Senguttuvel et al, 2010
RM493	TAGCTCCAACAGGATCGACC	GTACGTAAACGCGGAAGGTG	58	Monomorphic	Senguttuvel et al, 2010

Variable	K⁺/Na⁺ ratio	Na⁺ concentration	K⁺ concentration
Biomass	0.805*	-0.245	0.815*
K⁺/Na⁺ ratio		-0.552	0.758*
Na⁺ concentration			-0.068