Agrobacterium-Mediated Transformation of Rice: Constraints and Possible Solutions

doi:10.1016/j.rsci.2019.04.001

Abstract

Abstract:

Genetic transformation of rice (Oryza sativa L.) by introducing beneficial traits is now a central research instrument in plant physiology and a practical tool for plant improvement. Many approaches are verified for stable introduction of foreign genes into the plant genome. The review examined the different constraints that limit the success of rice genetic transformation via Agrobacterium-mediated approach and suggested possible solutions. Explant identification, gene transfer technique and construct to tailor the integration, transgene expression without collateral to genetic damage and transformant selection are among the technical challenges affecting the rice transformation. Despite the contests, Agrobacterium- mediated transformation system has been a better option for producing transgenic rice varieties because of its exact T-DNA processing and simple integration of low copy-number transgene. This information is necessary for improving the transformation system for recalcitrant rice varieties.

Key words: rice, Agrobacterium-mediated transformation, tissue culture, gene transfer, T-DNA integration

Mohammed Sulaiman, Abd Samad Azman, Rahmat Zaidah. Agrobacterium-Mediated Transformation of Rice: Constraints and Possible Solutions[J]. Rice Science, 2019, 26(3): 133-146.

Figures/Tables 4

References 136

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Cultivar	Sub-species	Medium ^a	Target explant	Improvement trait	Reference
Pusa Basmati	indica	MS	Embryogenic callus	Development of fertile transgenic rice	Mohanty et al, 1999
Sambha Mahsuri, Cotton Sannalu, Pusa Basmati, Taraori Basmati	indica	MS	Embryogenic callus	Abiotic stress (salt) resistant; SOD gene	Sarangi et al, 2011 Reddy et al, 2019
IR64	indica	MS	Shoot apex	Antibiotic resistant plant	Dey et al, 2012
Cempo Ireng	indica	2N6, MS	Embryogenic callus	Early flowering development	Purwestri et al, 2015
MR219	indica	MS	Embryogenic callus	Overexpression of stress related gene, Auxin Binding Protein 57 (Abp 57)	Tan et al, 2017
Sambha Mahsuri, Cotton Sannalu	indica	N6, MS	Embryogenic callus	Improvement of drought tolerance	Reddy et al, 2018
Tsukinohikari, Asanohikari, Koshihikari	japonica	N6	Immature embryo	Hygromycin resistant plant	Hiei et al, 1994
Nipponbare	japonica	N6	Embryogenic callus	Identification of HAP2 genes, 11 HAP3 genes and 7 HAP5 genes binding to the CCAAT-box	Thirumurugan et al, 2008
Nipponbare	japonica	MS	Embryogenic callus	Resistance to drought, salinity and pathogens and increasing photosynthesis potential and tiller number	Alam et al, 2015
Nipponbare	japonica	YN	Embryo	Heat tolerance	Liu et al, 2016
Dongjin	japonica	YN	Embryo	Increase tolerance to cold stress, OsCYP19-4 gene	Yoon et al, 2015
Dongjin	japonica	2N6	Embryogenic callus	Promote flowering in rice at short-day condition	Han et al, 2015
Taipei 309	japonica	MS, N6	Embryogenic callus	Iron vitamin improvement	Lucca et al, 2001
Taipei 309	japonica	MS	Embryogenic callus	Early development of rice inflorescence	Prasad et al, 2003
Zhonghua 11	japonica	N6	Immature embryo	Floral activator OsELF3 controlling heading date at long-day condition	Yang et al, 2013
Zhonghua 11	japonica	MS	Embryogenic callus	Increase plant height, grain yield and grain weight	Zhu et al, 2017

Cultivar	Sub-species	Medium ^a	Target explant	Improvement trait	Reference
Pusa Basmati	indica	MS	Embryogenic callus	Development of fertile transgenic rice	Mohanty et al, 1999
Sambha Mahsuri, Cotton Sannalu, Pusa Basmati, Taraori Basmati	indica	MS	Embryogenic callus	Abiotic stress (salt) resistant; SOD gene	Sarangi et al, 2011 Reddy et al, 2019
IR64	indica	MS	Shoot apex	Antibiotic resistant plant	Dey et al, 2012
Cempo Ireng	indica	2N6, MS	Embryogenic callus	Early flowering development	Purwestri et al, 2015
MR219	indica	MS	Embryogenic callus	Overexpression of stress related gene, Auxin Binding Protein 57 (Abp 57)	Tan et al, 2017
Sambha Mahsuri, Cotton Sannalu	indica	N6, MS	Embryogenic callus	Improvement of drought tolerance	Reddy et al, 2018
Tsukinohikari, Asanohikari, Koshihikari	japonica	N6	Immature embryo	Hygromycin resistant plant	Hiei et al, 1994
Nipponbare	japonica	N6	Embryogenic callus	Identification of HAP2 genes, 11 HAP3 genes and 7 HAP5 genes binding to the CCAAT-box	Thirumurugan et al, 2008
Nipponbare	japonica	MS	Embryogenic callus	Resistance to drought, salinity and pathogens and increasing photosynthesis potential and tiller number	Alam et al, 2015
Nipponbare	japonica	YN	Embryo	Heat tolerance	Liu et al, 2016
Dongjin	japonica	YN	Embryo	Increase tolerance to cold stress, OsCYP19-4 gene	Yoon et al, 2015
Dongjin	japonica	2N6	Embryogenic callus	Promote flowering in rice at short-day condition	Han et al, 2015
Taipei 309	japonica	MS, N6	Embryogenic callus	Iron vitamin improvement	Lucca et al, 2001
Taipei 309	japonica	MS	Embryogenic callus	Early development of rice inflorescence	Prasad et al, 2003
Zhonghua 11	japonica	N6	Immature embryo	Floral activator OsELF3 controlling heading date at long-day condition	Yang et al, 2013
Zhonghua 11	japonica	MS	Embryogenic callus	Increase plant height, grain yield and grain weight	Zhu et al, 2017

Variety	Sub-species	Agrobacterium strain	Reporter gene	Selectable marker	Transformation efficiency (%)	Reference
Kasalath	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	66.9	Saika and Toki, 2010
			CaMV35S:sgfp
			CaMV35S:luc
Pusa Basmati	indica	EHA105	CaMV35S:gus	CaMV35S:nptII	26	Tripathi et al, 2010
MR219	indica	LBA4404	CaMV35S:gus	CaMV35S:hpt	± 35	Rahman et al, 2011
MR219	indica	LBA4404	CaMV35S:gus	CaMV35S:nptII	± 35	Rahman et al, 2011
Handao 297	japonica	ALG1	CaMV35S:gus	CaMV35S:hpt	20	Zhao et al, 2011
Pusa Basmati, Taraori Basmati	indica	LBA4404	CaMV35S:gus	CaMV35S:hpt	80	Sarangi et al, 2011
IR64, CSR10, PB1, Swarna	indica	EHA105, LBA4404	CaMV35S:gus	CaMV35S:hpt	45	Sahoo et al, 2011
BR29, IR68899B	indica	EHA105	rd29	rd29:hpt	12	Datta et al, 2012
Zhenshan 97	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	85.2	Tie et al, 2012
Nipponbare	japonica	EHA105	CaMV35S:rUbi	CaMV35S:hpt	1.8	Ozawa et al, 2012
IR64	indica	LBA4404	CaMV35S:SUV	CaMV35S:hpt	12	Sahoo and Tuteja, 2012
BPT5204	indica	LBA4404	CaMV35S:gus	CaMV35S:hpt	16.7	Manimaran et al, 2013
BPT5204	indica	LBA4404	CaMV35S:gus	CaMV35S:nptII	16.7	Manimaran et al, 2013
IR36	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	99.5	Krishnan et al, 2013
Zhonghua 11	japonica	EHA105	CaMV35S:gus	CaMV35S:hpt	66.7	Yang et al, 2013
Samba Mahsuri (BPT-5204)	indica	LBA4404	CaMV35S:rd29A	CaMV35S:hpt	± 30	Ravikumar et al, 2014
MR219	indica	EHA101, EHA105, LBA4404	mgfp:gus	mGFP:hptII	5.8	Fook et al, 2015
JK1044R, JKRH401	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	30	Chakraborty et al, 2016
Nipponbare	japonica	EHA105	Ubi promoter	Ubi:hpt	41.2	Xu et al, 2017
Zhonghua 11	japonica	EHA105	CaMV35S:gus	CaMV35S:hpt	± 67.4	Zhu et al, 2017
SR1, Jhelum, K332	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	9.3	Yaqoob et al, 2017
SR1, Jhelum, K332	indica	EHA105	CaMV35S:gus	CaMV35S:nptII	9.3	Yaqoob et al, 2017
MR219	indica	LBA4404	CaMV35S	CaMV35S:hptII	26.4	Tan et al, 2017
Sambha Mahsuri, Cotton Sannula	indica	LBA4404	CaMV35S:gus	CaMV35S:nptII	± 50	Reddy et al, 2019

Variety	Sub-species	Agrobacterium strain	Reporter gene	Selectable marker	Transformation efficiency (%)	Reference
Kasalath	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	66.9	Saika and Toki, 2010
			CaMV35S:sgfp
			CaMV35S:luc
Pusa Basmati	indica	EHA105	CaMV35S:gus	CaMV35S:nptII	26	Tripathi et al, 2010
MR219	indica	LBA4404	CaMV35S:gus	CaMV35S:hpt	± 35	Rahman et al, 2011
MR219	indica	LBA4404	CaMV35S:gus	CaMV35S:nptII	± 35	Rahman et al, 2011
Handao 297	japonica	ALG1	CaMV35S:gus	CaMV35S:hpt	20	Zhao et al, 2011
Pusa Basmati, Taraori Basmati	indica	LBA4404	CaMV35S:gus	CaMV35S:hpt	80	Sarangi et al, 2011
IR64, CSR10, PB1, Swarna	indica	EHA105, LBA4404	CaMV35S:gus	CaMV35S:hpt	45	Sahoo et al, 2011
BR29, IR68899B	indica	EHA105	rd29	rd29:hpt	12	Datta et al, 2012
Zhenshan 97	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	85.2	Tie et al, 2012
Nipponbare	japonica	EHA105	CaMV35S:rUbi	CaMV35S:hpt	1.8	Ozawa et al, 2012
IR64	indica	LBA4404	CaMV35S:SUV	CaMV35S:hpt	12	Sahoo and Tuteja, 2012
BPT5204	indica	LBA4404	CaMV35S:gus	CaMV35S:hpt	16.7	Manimaran et al, 2013
BPT5204	indica	LBA4404	CaMV35S:gus	CaMV35S:nptII	16.7	Manimaran et al, 2013
IR36	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	99.5	Krishnan et al, 2013
Zhonghua 11	japonica	EHA105	CaMV35S:gus	CaMV35S:hpt	66.7	Yang et al, 2013
Samba Mahsuri (BPT-5204)	indica	LBA4404	CaMV35S:rd29A	CaMV35S:hpt	± 30	Ravikumar et al, 2014
MR219	indica	EHA101, EHA105, LBA4404	mgfp:gus	mGFP:hptII	5.8	Fook et al, 2015
JK1044R, JKRH401	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	30	Chakraborty et al, 2016
Nipponbare	japonica	EHA105	Ubi promoter	Ubi:hpt	41.2	Xu et al, 2017
Zhonghua 11	japonica	EHA105	CaMV35S:gus	CaMV35S:hpt	± 67.4	Zhu et al, 2017
SR1, Jhelum, K332	indica	EHA105	CaMV35S:gus	CaMV35S:hpt	9.3	Yaqoob et al, 2017
SR1, Jhelum, K332	indica	EHA105	CaMV35S:gus	CaMV35S:nptII	9.3	Yaqoob et al, 2017
MR219	indica	LBA4404	CaMV35S	CaMV35S:hptII	26.4	Tan et al, 2017
Sambha Mahsuri, Cotton Sannula	indica	LBA4404	CaMV35S:gus	CaMV35S:nptII	± 50	Reddy et al, 2019