Expression of a Magnaporthe grisea Elicitor and Its Biological Function in Activating Resistance in Rice

Abstract

Abstract: The expression of a protein elicitor from Magnaporthe griesea and its biological function in activating resistance in rice (Oryza sativa L) were reported. The gene of elicitor was expressed in Escherichia coli cells and produced a His6-fusion protein with 42 kD apparent molecular weight on SDS-PAGE. The purified protein could induce the resistance to blast disease, with the control efficiency of 46.47% and 36.41% at the 14th day and the 21st day after blast inoculation, respectively.After treatment with the expressed protein, the phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities were promoted in rice plants, meanwhile, the transcription levels of STKM, FAD, PBZ1 and PR1 genes were increased in rice plants. Moreover, after comparing the profile of total rice leaf proteins on two-dimensional electrophoresis gel, about 14 proteins were found to be increased in expression level after the expressed protein treatment. All the results indicated that the expressed protein could act as an elicitor to trigger the resistance in rice.

Key words: elicitor, Magnaporthe grisea, induced resistance, protein expression, blast resistance, rice, biological control

YAO Qing, YANG Xiu-fen, LIANG Ying, XU Feng, LIU Zheng, YUAN Jing-jing, QIU De-wen. Expression of a Magnaporthe grisea Elicitor and Its Biological Function in Activating Resistance in Rice[J]. RICE SCIENCE, 2007, 14(2): 149-156 .

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