Marker-Assisted Pyramiding of Genes Conferring Resistance Against Bacterial Blight and Blast Diseases into Indian Rice Variety MTU1010

doi:10.1016/j.rsci.2016.04.005

Abstract

Abstract:

Abstract: Two major bacterial blight (BB) resistance genes (Xa21 and xa13) and a major gene for blast resistance (Pi54) were introgressed into an Indian rice variety MTU1010 through marker-assisted backcross breeding. Improved Samba Mahsuri (possessing Xa21 and xa13) and NLR145 (possessing Pi54) were used as donor parents. Marker-assisted backcrossing was continued till BC₂ generation wherein PCR based functional markers specific for the resistance genes were used for foreground selection and a set of parental polymorphic microsatellite markers were used for background selection at each stage of backcrossing. Selected BC₂F₁ plants from both crosses, having the highest recoveries of MTU1010 genome (90% and 92%, respectively), were intercrossed to obtain intercross F₁(ICF₁) plants, which were then selfed to generate 880 ICF₂ plants possessing different combinations of the BB and blast resistance genes. Among the ICF₂plants, seven triple homozygous plants (xa13xa13Xa21Xa21Pi54Pi54) with recurrent parent genome recovery ranging from 82% to 92% were identified. All the seven ICF₂ plants showed high resistance against the bacterial blight disease with a lesion lengths of only 0.53-2.28 cm, 1%-5% disease leaf areas and disease scoring values of ‘1’ or ‘3’. The seven ICF₂plants were selfed to generate ICF₃, which were then screened for blast resistance, and all were observed to be highly resistant to the diseases. Several ICF₃ lines possessing high level of resistance against BB and blast, coupled with yield, grain quality and plant type on par with MTU1010 were identified and advanced for further selection and evaluation.

Key words: gene pyramiding, bacterial blight resistance, blast resistance, rice, marker-assisted backcross breeding

Arunakumari K., V. Durgarani C., Satturu V., R. Sarikonda K., D. R. Chittoor P., Vutukuri B., S. Laha G., P. K. Nelli A., Gattu S., Jamal M., Prasadbabu A., Hajira S., M. Sundaram R.. Marker-Assisted Pyramiding of Genes Conferring Resistance Against Bacterial Blight and Blast Diseases into Indian Rice Variety MTU1010[J]. Rice Science, 2016, 23(6): 306-316.

Figures/Tables 5

References 39

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Cross combination	Particular of cross combination	No. of plants screened	No. of plants confirmed	Gene combination in the confirmed plants
MTU1010 × ISM (C1)	C1-F₁	125	101	Xa13xa13Xa21xa21
MTU1010 × C1-F₁	C1-BC₁F₁	293	8	Xa13xa13Xa21xa21
MTU1010 × C1-BC₁F₁	C1-BC₂F₁	534	11	Xa13xa13Xa21xa21
MTU1010 × NLR145 (C2)	C2-F₁	110	74	Pi54pi54
MTU1010 × C2-F₁	C2-BC₁F₁	80	35	Pi54pi54
MTU1010 × C2-BC₁F₁	C2-BC₂F₁	268	17	Pi54pi54
C1-BC₂F₁ × C2-BC₂F₁	ICF₁	360	4	Xa13xa13Xa21xa21Pi54pi54
Selfed progeny of selected ICF₁ plant	ICF₂	880	7	xa13xa13Xa21Xa21Pi54Pi54
Selfed progeny of ICF₂	ICF₃	-	7	xa13xa13Xa21Xa21Pi54Pi54

Cross combination	Particular of cross combination	No. of plants screened	No. of plants confirmed	Gene combination in the confirmed plants
MTU1010 × ISM (C1)	C1-F₁	125	101	Xa13xa13Xa21xa21
MTU1010 × C1-F₁	C1-BC₁F₁	293	8	Xa13xa13Xa21xa21
MTU1010 × C1-BC₁F₁	C1-BC₂F₁	534	11	Xa13xa13Xa21xa21
MTU1010 × NLR145 (C2)	C2-F₁	110	74	Pi54pi54
MTU1010 × C2-F₁	C2-BC₁F₁	80	35	Pi54pi54
MTU1010 × C2-BC₁F₁	C2-BC₂F₁	268	17	Pi54pi54
C1-BC₂F₁ × C2-BC₂F₁	ICF₁	360	4	Xa13xa13Xa21xa21Pi54pi54
Selfed progeny of selected ICF₁ plant	ICF₂	880	7	xa13xa13Xa21Xa21Pi54Pi54
Selfed progeny of ICF₂	ICF₃	-	7	xa13xa13Xa21Xa21Pi54Pi54

Plant identity	Allelic status of xa13, Xa21 and Pi54	Disease scoring scale for BB in ICF₂	Disease scoring scale for rice blast in ICF₃	Background genome recovery (%)
MTU1010	Xa13Xa13xa21xa21pi54pi54	9	7	-
Improved Samba Mahsuri	xa13xa13Xa21Xa21	1	-	-
NLR145	Pi54Pi54	-	3	-
ICF_2:3-16-59	xa13xa13Xa21Xa21Pi54Pi54	1	1	92
ICF_2:3-16-231	xa13xa13Xa21Xa21Pi54Pi54	1	2	85
ICF_2:3-16-235	xa13xa13Xa21Xa21Pi54Pi54	1	2	82
ICF_2:3-16-282	xa13xa13Xa21Xa21Pi54Pi54	1	2	83
ICF_2:3-16-521	xa13xa13Xa21Xa21Pi54Pi54	1	1	82
ICF_2:3-16-786	xa13xa13Xa21Xa21Pi54Pi54	3	2	83
ICF_2:3-16-837	xa13xa13Xa21Xa21Pi54Pi54	1	2	88

Plant identity	Allelic status of xa13, Xa21 and Pi54	Disease scoring scale for BB in ICF₂	Disease scoring scale for rice blast in ICF₃	Background genome recovery (%)
MTU1010	Xa13Xa13xa21xa21pi54pi54	9	7	-
Improved Samba Mahsuri	xa13xa13Xa21Xa21	1	-	-
NLR145	Pi54Pi54	-	3	-
ICF_2:3-16-59	xa13xa13Xa21Xa21Pi54Pi54	1	1	92
ICF_2:3-16-231	xa13xa13Xa21Xa21Pi54Pi54	1	2	85
ICF_2:3-16-235	xa13xa13Xa21Xa21Pi54Pi54	1	2	82
ICF_2:3-16-282	xa13xa13Xa21Xa21Pi54Pi54	1	2	83
ICF_2:3-16-521	xa13xa13Xa21Xa21Pi54Pi54	1	1	82
ICF_2:3-16-786	xa13xa13Xa21Xa21Pi54Pi54	3	2	83
ICF_2:3-16-837	xa13xa13Xa21Xa21Pi54Pi54	1	2	88

Line	Days to heading (d)	Plant height (cm)	No. of productive panicles per plant	Panicle length (cm)	No. of filled grains per panicle	Grain yield per plant (g)	1000-grain weight (g)	Grain type
ICF₃-16-59	87.3	106.0	15.7	31.3*	118.3	24.0	16.3	LS
ICF₃-16-231	83.6	104.0	17.0	30.3*	111.7	24.7	16.5	LS
ICF₃-16-235	86.0	105.7	18.7	29.0*	130.0*	27.0*	17.1	LS
ICF₃-16-282	85.3	107.3	17.7	28.7	114.7	24.3	16.5	LS
ICF₃-16-521	85.7	106.3	14.7	27.7	110.3	24.0	16.4	LS
ICF₃-16-786	87.7	107.0	13.3	28.0	101.7	23.3	16.8	LS
ICF₃-16-837	88.0	103.7	16.7	26.7	108.0	23.7	16.7	LS
MTU1010	86.7	107.3	16.7	26.0	117.7	23.7	16.4	LS
SD	1.4	1.4	1.7	1.6	8.4	1.1	0.3
CV (%)	1.7	1.3	10.4	6.2	7.4	4.4	1.6
CD (5%)	1.4	2.7	2.0	2.0	2.6	1.0	0.1
SD, Standard deviation; CV, Coefficient of variation; CD, Critical difference; LS, Long slender grain type. *, Values significantly different from MTU1010 at the 0.05 level.