Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines

doi:10.1016/j.rsci.2020.08.001

Abstract

Abstract:

Improved rice lines were developed frome three parents with the resistance or tolerance to bacterial leaf blight, blast and drought stress, respectively, using single-, double- and three-way crosses. The improved lines were assessed for agro-morphological and yield traits under non-drought stress (NS) and reproductive-stage drought stress (RS) treatments. The mean comparison of traits measured between parent plants and progenies (improved lines) were similar, and there were significant and non-significant differences among the parents and improved lines (genotypes) under NS and RS. Smilarly, there was significant and non-significant differences in the interaction among both parent varieties and improved lines for NS and RS. Cluster and 3D-model of principal component analysis did not generate categorical clusters according to crossing methods, and there were no exclusive crossing method inclined variations under the treatments. The improved lines were high-yielding, disease resistant, and drought-tolerant compared with their parents. All the crossing methods were good for this crop improvement program without preference to any, despite the number of genes introgressed.

Key words: rice, bacteria leaf blight, blast, drought tolerance, resistance gene

Silas Akos Ibrahim, Y. Rafii Mohd, Razi Ismail Mohd, Izan Ramlee Shairul, Abd Aziz Shamsudin Noraziyah, Ramli Asfaliza, Chibuike Chukwu Samuel, Swaray Senesie, Jalloh Momodu. Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines[J]. Rice Science, 2021, 28(3): 279-288.

Figures/Tables 9

References 50

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Genotype	Blast resistance gene				BLB resistance gene					Drought tolerance QTL
	Pi9	Pi2	Piz		Xa4		xa5	xa13	Xa21	qDTY_2.2		qDTY_3.2	qDTY_12.1
	RM6836	RM6836	RM6836/ RM8225		RM224		RM122/ RM13	Xa13prom/ RG136	RM21/ pTA248	RM236		RM520	RM511/ RM1261
Putra-1 (P)^a	+	+		+		-	-	-	-		-	-	-
IRBB60 (B)^a	-	-		-		+	+	+	+		-	-	-
MR219-PL-137 (D)^a	-	-		-		-	-	-	-		+	+	+
PD14	+	+		+		-,,	-	-	-		+	+	+
PD15	+	+		+		-	-	-	-		+	+	+
PB12	+	+		+		+	+	+	+		-	-	-
PB14	+	+		+		+	+	+	+		-	-	-
PBD1	+	+		+		+	+	+	+		+	+	+
PBD3	+	+		+		+	+	+	+		+	+	+
PDB3	+	+		+		+	+	+	+		+	+	+
DPB7	+	+		+		+	+	+	+		+	+	+
DPB12	+	+		+		+	+	+	+		-	+	+
DPB13	+	+		+		+	+	+	+		+	+	+
DPB20	+	+		+		+	+	+	+		+	+	+

Genotype	Blast resistance gene				BLB resistance gene					Drought tolerance QTL
	Pi9	Pi2	Piz		Xa4		xa5	xa13	Xa21	qDTY_2.2		qDTY_3.2	qDTY_12.1
	RM6836	RM6836	RM6836/ RM8225		RM224		RM122/ RM13	Xa13prom/ RG136	RM21/ pTA248	RM236		RM520	RM511/ RM1261
Putra-1 (P)^a	+	+		+		-	-	-	-		-	-	-
IRBB60 (B)^a	-	-		-		+	+	+	+		-	-	-
MR219-PL-137 (D)^a	-	-		-		-	-	-	-		+	+	+
PD14	+	+		+		-,,	-	-	-		+	+	+
PD15	+	+		+		-	-	-	-		+	+	+
PB12	+	+		+		+	+	+	+		-	-	-
PB14	+	+		+		+	+	+	+		-	-	-
PBD1	+	+		+		+	+	+	+		+	+	+
PBD3	+	+		+		+	+	+	+		+	+	+
PDB3	+	+		+		+	+	+	+		+	+	+
DPB7	+	+		+		+	+	+	+		+	+	+
DPB12	+	+		+		+	+	+	+		-	+	+
DPB13	+	+		+		+	+	+	+		+	+	+
DPB20	+	+		+		+	+	+	+		+	+	+

Crossing method	Population code	Lines developed	Introgressed genotype
Single cross	PD	PD14, PD15	Putra-1 × MR219-PL-137
Single cross	PB	PB12, PB14	Putra-1 × IRBB60
Double cross	PDB	PDB3	Putra-1 × MR219-PL-137 × IRBB60
Three-way cross	PBD	PBD1, PBD3	Putra-1× IRBB60 × MR219-PL-137
Three-way reciprocal cross	DPB	DPB7, DPB12, DPB13, DPB20	MR219-PL-137 × Putra-1 × IRBB60

Crossing method	Population code	Lines developed	Introgressed genotype
Single cross	PD	PD14, PD15	Putra-1 × MR219-PL-137
Single cross	PB	PB12, PB14	Putra-1 × IRBB60
Double cross	PDB	PDB3	Putra-1 × MR219-PL-137 × IRBB60
Three-way cross	PBD	PBD1, PBD3	Putra-1× IRBB60 × MR219-PL-137
Three-way reciprocal cross	DPB	DPB7, DPB12, DPB13, DPB20	MR219-PL-137 × Putra-1 × IRBB60

Genotype	DTF (d)									PH (cm)											PL (cm)
Genotype	NS			RS			Pool		NS				RS			Pool					NS						RS				Pool
P	89.0 ± 0.7 a			NA			NA		103.2 ± 1.1 c				NA			NA					25.0 ± 0.5 ab					NA					NA
D	88.0 ± 0.7 ab			NA			NA		100.3 ± 1.5 c				NA			NA					25.9 ± 0.3 ab					NA					NA
B	87.8 ± 0.7 a-c			96.6 ± 0.6 a			92.2 ± 1.5 a		103.6 ± 1.6 c				97.5 ± 3.7 a			100.6 ± 2.2 b					25.1 ± 0.1 b					20.7 ± 0.9 c					22.9 ± 0.9 c
PB12	87.6 ± 0.4 a-c			NA			NA		104.3 ± 2.3 c				NA			NA					24.9 ± 0.1 b					NA					NA
PB15	86.8 ± 0.7 a-d			NA			NA		102.6 ± 1.8 c				NA			NA					25.9 ± 0.4 ab					NA					NA
PD14	85.8 ± 0.5 b-d			91.8 ± 0.4 d			88.8 ± 1.0 de		102.6 ± 2.3 c				96.7 ± 4.1 a			99.6 ± 2.4 b					25.3 ± 0.4 b					23.7 ± 0.5 ab					24.5 ± 0.4 ab
PD15	87.4 ± 0.5 a-d			92.8 ± 0.5 cd			90.1 ± 1.0 b-d		104.5 ± 2.8 c				97.3 ± 4.0 a			100.9 ± 2.6 b					24.7 ± 0.4 b					22.6 ± 0.5 ab					23.6 ± 0.5 bc
PBD1	87.6 ± 0.5 a-c			94.0 ± 0.6 bc			90.8 ± 1.1 a-c		104.7 ± 2.4 c				100.5 ± 3.8 a			102.6 ± 2.3 ab					24.9 ± 0.6 b					22.1 ± 0.5 bc					23.5 ± 0.6 bc
PBD3	88.0 ± 0.5 ab			94.0 ± 0.5 bc			91.0 ± 1.0 ab		99.9 ± 0.3 c				96.2 ± 4.4 a			98.1 ± 2.7 b					22.9 ± 0.6 c					23.3 ± 0.5 ab					24.5 ± 0.4 ab
PDB3	87.4 ± 0.5 a-d			92.8 ± 0.4 cd			90.1 ± 1.0 b-d		104.1 ± 3.0 c				100.6 ± 4.3 a			102.3 ± 2.6 ab					24.9 ± 0.4 b					24.1 ± 0.4 a					24.5 ± 0.3 ab
DPB7	85.2 ± 1.7 d			91.8 ± 0.4 d			88.5 ± 1.4 e		113.4 ± 2.1 a				102.1 ± 4.3 a			107.8 ± 2.9 a					25.0 ± 0.5 b					24.0 ± 1.0 a					24.5 ± 0.5 ab
DPB12	86.0 ± 1.0 b-d			92.8 ± 0.6 cd			89.4 ± 1.3 c-e		106.1 ± 1.1 bc				99.7 ± 3.5 a			102.9 ± 2.0 ab					25.0 ± 0.4 b					23.4 ± 0.3 ab					24.2 ± 0.4 ab
DPB13	86.2 ± 0.9 b-d			93.0 ± 0.7 d			89.6 ± 1.3 b-e		103.6 ± 2.4 c				98.4 ± 3.7 a			101.0 ± 2.2 b					25.2 ± 0.4 b					23.4 ± 0.3 ab					24.3 ± 0.4 ab
DPB20	85.6 ± 1.0 cd			95.0 ± 0.7 b			90.3 ± 1.7 bc		112.3 ± 3.0 ab				96.3 ± 4.9 a			104.3 ± 3.8 ab					26.6 ± 0.2 a					23.7 ± 0.6 ab					25.2 ± 0.6 a
Mean	87.03			93.46			90.08		104.67				98.54			102.01					25.09					23.09					24.02
CV (%)	2.03			1.30			1.79		4.74				9.35			7.28					4.07					5.87					5.05
LSD	2.25			1.56			1.43		6.29				11.82			6.61					1.30					1.74					1.08
h²_B(%)	66.27			88.09			75.05		28.63				20.22			76.52					31.13					74.83					4.76
Genotype	ET								TT									FFG
Genotype	NS	RS			Pool				NS		RS			Pool				NS				RS						Pool
P	10.2 ± 1.4 b-d	NA			NA				10.6 ± 1.6 b-e		NA			NA			172.8 ± 5.9 ab						NA					NA
D	13.6 ± 1.8 a	NA			NA				13.8 ± 1.7 a		NA			NA			168.4 ± 4.0 a-c						NA					NA
B	7.8 ± 1.3 de	9.0 ± 1.6 ab			8.4 ± 1.0 c				8.8 ± 1.0 de		9.6 ± 1.4 ab			9.2 ± 0.8 b/d			172.4 ± 9.3 ab						26.4 ± 2.9 c					99.4 d ± 24.8 e
PB12	9.4 ± 0.5 b-e	NA			NA				9.6 ± 0.5 c-e		NA			NA			177.6 ± 6.6 a						NA					NA
PB15	9.0 ± 1.1 c-e	NA			NA				9.2 ± 0.9 de		NA			NA			166.0 ± 5.8 a-d						NA					NA
PD14	10.6 ± 0.4 b-d	11.4 ± 1.2 a			11.0 ± 0.6 a				11.0 ± 0.5 a-d		11.4 ± 1.2 ab			11.2 ± 0.6 ab			178.4 ± 8.8 a						52.4 ± 2.3 ab					115.4 ± 21.4 a
PD15	9.6 ± 1.2 b-e	8.0 ± 1.4 b			8.8 ± 0.9 bc				11.0 ± 1.1 a-d		8.4 ± 1.2 b			9.7 ± 0.9 b-d			157.2 ± 1.2 cd						49.8 ± 5.2 ab					103.5 ± 18.1 cd
PBD1	11.0 ± 1.0 a-c	11.6 ± 0.5 a			11.3 ± 0.5 a				12.4 ± 1.5 a-c		11.6 ± 0.5 a			12.0 ± 0.8 a			170.6 ± 3.7 a-c						45.4 ± 2.8 b					108.0 ± 21.0 a-c
PBD3	12.0 ± 0.6 ab	10.6 ± 1.2 ab			11.3 ± 0.7 a				13.2 ± 0.9 ab		10.6 ± 1.2 ab			11.5 ± 0.8 ab			168.4 ± 2.7 a-c						59.4 ± 5.9 a					113.9 ± 18.4 ab
PDB3	11.2 ± 1.1 a-c	10.0 ± 1.3 ab			10.6 ± 0.8 ab				13.0 ± 1.1 ab		10.0 ± 1.3 ab			11.5 ± 1.0 ab			153.4 ± 2.0 d						48.2 ± 1.6 ab					100.8 ± 17.6 cd
DPB7	9.4 ± 0.5 b-e	11.8 ± 0.7 a			10.6 ± 0.6 ab				9.8 ± 0.5 c-e		11.8 ± 0.7 a			10.8 ± 0.5 a-c			161.4 ± 3.1 b-d						53.4 ± 3.8 ab					107.4 ± 18.2 a-d
DPB12	7.2 ± 0.6 e	9.2 ± 1.2 ab			8.2 ± 0.7 c				7.8 ± 0.5 e		9.4 ± 1.0 ab			8.6 ± 0.6 d			152.4 ± 1.7 d						52.2 ± 4.9 ab					102.3 ± 16.9 cd
DPB13	9.4 ± 0.5 c-e	10.2 ± 1.2 ab			9.8 ± 0.7 a-c				9.6 ± 0.6 c-e		11.4 ± 1.0 ab			10.5 ± 0.6 a-d			161.0 ± 3.2 b-d						43.8 ± 5.5 b					102.4 ± 19.8 cd
DPB20	9.0 ± 1.0 c-e	10.2 ± 1.1 ab			9.6 ± 0.7 a-c				9.2 ± 1.0 de		10.4 ± 1.0 ab			9.8 ± 0.7 b-d			165.8 ± 3.6 a-d						46.2 ± 4.0 b					106.0 ± 20.1 b-d
Mean	9.96	10.20			9.96				10.64		10.46			10.52			166.13						47.72					105.91
CV (%)	23.13	25.43			22.97				21.69		22.86			21.06			6.54						19.30					9.11
LSD	2.92	3.33			2.04				2.93		3.07			1.97			13.78						11.81					8.60
h²_B(%)	72.11	52.61			14.31				76.02		52.09			7.79			74.06						81.94					0
Genotype	HGW (g)									GLWR										GD (d)
Genotype	NS		RS			Pool				NS		RS			Pool					NS					RS					Pool
P	2.41 ± 0.03 de		NA			NA		4.97 ± 0.07bc				NA			NA				117.4 ± 0.2 ab					NA					NA
D	2.47 ± 0.08 c-e		NA			NA		4.98 ± 0.11 b				NA			NA				117.4 ± 0.2 ab					NA					NA
B	2.35 ± 0.07 e		2.43 ± 0.06 a			2.39 ± 0.04 c		5.61 ± 0.23 a				4.42 ± 0.04 b			5.01 ± 0.23 a				116.8 ± 0.2 b					133.2 ± 1.2 b					125.0 ± 2.8 a
PB12	2.55 ± 0.03 a-c		NA			NA		4.96 ± 0.02 bc				NA			NA				118.2 ± 0.4 a					NA					NA
PB15	2.49 c-e		NA			NA		4.81 b-d				NA			NA				117.6 ab					NA					NA
PD14	2.55 ± 0.23 a-c		2.34 ± 0.03 a			2.44 ± 0.04 bc		4.86 ± 0.04 b-d				4.49 ± 0.04 b			4.67 ± 0.07 b				117.4 ± 0.5 ab					128.4 ± 0.2 b					122.9 ± 1.9 b
PD15	2.52 ± 0.02 a-d		2.40 ± 0.05 a			2.46 ± 0.03 a-c		4.86 ± 0.04 b-d				4.49 ± 0.02 b			4.67 ± 0.06 b				117.6 ± 0.5 ab					127.6 ± 0.5 b					122.6 ± 1.7 b
PBD1	2.63 ± 0.04 a		2.42 ± 0.05 a			2.52 ± 0.05 a		4.59 ± 0.04 b-d				4.51 ± 0.01 b			4.55 ± 0.03 bc				117.6 ± 0.4 ab					127.8 ± 0.4b					122.7 ± 1.7 b
PBD3	2.52 ± 0.04 a-d		2.38 ± 0.05 a			2.45 ± 0.04 a-c		4.63 ± 0.08 b-d				4.47 ± 0.04 b			4.55 ± 0.05 b				117.6 ± 0.5 ab					129.0 ± 0.5 b					123.3 ± 1.9 b
PDB3	2.47 ± 0.04 c-e		2.41 ± 0.03 a			2.44 ± 0.02 bc		4.53 ± 0.02 d				4.50 ± 0.02 b			4.51 ± 0.02 bc				117.0 ± 0.6 ab					127.8 ± 0.6 b					122.4 ± 1.8 b
DPB7	2.52 ± 0.06 a-d		2.32 ± 0.02 a			2.42 ± 0.05 bc		4.51 ± 0.04 cd				4.57 ± 0.03 ab			4.54 ± 0.02 bc				117.0 ± 0.7 ab					127.4 ± 0.5 ab					122.2 ± 1.8 b
DPB12	2.52 ± 0.03 a-d		2.35 ± 0.03 a			2.43 ± 0.04 bc		4.57 ± 0.04 cd				4.75 ± 0.20 a			4.66 ± 0.10 b				117.6 ± 0.9 ab					128.2 ± 0.4 a					122.9 ± 1.8 b
DPB13	2.60 ± 0.03 ab		2.39 ± 0.03 a			2.49 ± 0.04 ab		4.51 ± 0.04 d				4.57 ± 0.03 ab			4.54 ± 0.02 bc				117.0 ± 0.7 ab					128.2 ± 0.6 ab					122.6 ± 1.9 b
DPB20	2.56 ± 0.03 a-c		2.43 ± 0.02 a			2.50 ± 0.03 ab		4.08 ± 0.43 e				4.53 ± 0.05 b			4.31 ± 0.22 c				117.8 ± 0.4 ab					127.6 ± 0.7 b					122.7 ± 1.7 b
Mean	2.51		2.39			2.45		4.75				4.53			4.60				117.43					128.52					122.93
CV	3.83		3.75			3.67		6.63				3.50			5.99				0.88					1.09					1.03
LSD	0.12		0.11			0.08		0.40				0.20			0.25				1.31					1.80					1.12
h²_B(%)	66.67		50.00			0		85.92				57.14			0				39.77					88.13					0