Rice Science ›› 2020, Vol. 27 ›› Issue (6): 515-528.DOI: 10.1016/j.rsci.2020.09.008
• Research Paper • Previous Articles Next Articles
Mondal Satyen1,2,3(
), Jamil Hasan M.2, Ahmed Tofayel4, Giashuddin Miah M.4, C. Sta Cruz Pompe3, M. Ismail Abdel1()
Received:2019-08-13
Accepted:2019-12-09
Online:2020-11-28
Published:2020-11-28
Mondal Satyen, Jamil Hasan M., Ahmed Tofayel, Giashuddin Miah M., C. Sta Cruz Pompe, M. Ismail Abdel. Effects of AG1 and AG2 QTLs on Nonstructural Carbohydrate and Seed Management Options for Rice Seedling Growth and Establishment under Flooding Stress[J]. Rice Science, 2020, 27(6): 515-528.
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| Carbohydrate | Genotype | Flooded | Control | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 DAS | 1 DAS | 2 DAS | 3 DAS | 7 DAS | 0 DAS | 1 DAS | 2 DAS | 3 DAS | 7 DAS | |||
| Soluble sugar | PSB Rc82-AG1 | 1.1 | 1.1 | 1.1 | 1.6 | 2.0 | 1.1 | 1.5 | 1.8 | 2.3 | 3.1 | |
| PSB Rc82-AG2 | 1.1 | 1.1 | 1.1 | 1.7 | 2.1 | 1.1 | 1.5 | 1.8 | 2.3 | 3.2 | ||
| PSB Rc82-AG1-AG2 | 1.1 | 1.2 | 1.3 | 1.9 | 2.3 | 1.1 | 1.5 | 1.8 | 2.3 | 3.2 | ||
| Ciherang-Sub1-AG1 | 1.1 | 1.1 | 1.1 | 1.6 | 2.1 | 1.1 | 1.5 | 1.9 | 2.3 | 3.3 | ||
| Ciherang-Sub1-AG2 | 1.1 | 1.2 | 1.1 | 1.8 | 2.4 | 1.1 | 1.5 | 1.9 | 2.3 | 3.3 | ||
| Ciherang-Sub1-AG1-AG2 | 1.1 | 1.2 | 1.2 | 1.9 | 2.5 | 1.1 | 1.6 | 2.0 | 2.4 | 3.3 | ||
| PSB Rc82 | 0.9 | 0.9 | 0.9 | 0.9 | 1.0 | 0.9 | 1.7 | 1.9 | 2.6 | 3.2 | ||
| Ciherang-Sub1 | 1.0 | 1.0 | 1.0 | 1.0 | 1.4 | 1.0 | 1.6 | 2.0 | 2.5 | 3.3 | ||
| Kho Hlan On | 1.2 | 1.2 | 1.2 | 2.1 | 3.0 | 1.2 | 1.6 | 2.0 | 2.5 | 3.3 | ||
| Ma-Zhan Red | 1.2 | 1.2 | 1.2 | 1.9 | 2.7 | 1.2 | 1.5 | 2.0 | 2.5 | 3.3 | ||
| Mean | 1.1 | 1.1 | 1.1 | 1.6 | 2.2 | 1.1 | 1.6 | 1.9 | 2.4 | 3.3 | ||
| HSD0.05 | ns | 0.20*** | 0.19*** | 0.31*** | 0.37*** | ns | ns | ns | 0.31*** | ns | ||
| Starch | PSB Rc82-AG1 | 74.5 | 72.8 | 69.9 | 66.9 | 64.0 | 74.5 | 69.1 | 63.1 | 56.1 | 24.5 | |
| PSB Rc82-AG2 | 77.6 | 74.2 | 66.8 | 64.0 | 61.1 | 77.6 | 71.4 | 62.9 | 56.4 | 23.4 | ||
| PSB Rc82-AG1-AG2 | 75.8 | 72.9 | 70.3 | 66.5 | 60.2 | 75.8 | 69.9 | 64.6 | 54.7 | 22.4 | ||
| Ciherang-Sub1-AG1 | 75.3 | 72.9 | 69.4 | 66.6 | 62.0 | 75.3 | 72.3 | 65.6 | 57.8 | 25.3 | ||
| Ciherang-Sub1-AG2 | 77.5 | 75.0 | 71.2 | 66.0 | 60.6 | 77.5 | 69.6 | 63.7 | 55.4 | 16.5 | ||
| Ciherang-Sub1-AG1-AG2 | 76.7 | 73.8 | 69.1 | 65.7 | 59.8 | 76.7 | 71.3 | 63.9 | 58.0 | 17.8 | ||
| PSB Rc82 | 75.8 | 74.5 | 74.0 | 72.1 | 68.1 | 75.8 | 70.3 | 63.0 | 57.0 | 27.1 | ||
| Ciherang-Sub1 | 74.5 | 72.9 | 72.3 | 69.6 | 65.8 | 74.5 | 72.0 | 66.1 | 56.4 | 20.7 | ||
| Kho Hlan On | 78.9 | 73.2 | 70.2 | 65.4 | 59.0 | 78.9 | 72.6 | 66.5 | 55.5 | 17.1 | ||
| Ma-Zhan Red | 76.1 | 71.3 | 70.5 | 65.5 | 60.0 | 76.1 | 68.8 | 63.0 | 58.0 | 15.2 | ||
| Mean | 76.3 | 73.4 | 70.4 | 66.8 | 62.1 | 76.3 | 70.7 | 64.2 | 56.5 | 21.0 | ||
| HSD0.05 | ns | ns | ns | ns | 5.23*** | ns | ns | ns | ns | ns | ||
Table 1 Soluble sugar and starch concentrations in germinating seeds under flooded with 10 cm water depth and control conditions in greenhouse. %
| Carbohydrate | Genotype | Flooded | Control | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 DAS | 1 DAS | 2 DAS | 3 DAS | 7 DAS | 0 DAS | 1 DAS | 2 DAS | 3 DAS | 7 DAS | |||
| Soluble sugar | PSB Rc82-AG1 | 1.1 | 1.1 | 1.1 | 1.6 | 2.0 | 1.1 | 1.5 | 1.8 | 2.3 | 3.1 | |
| PSB Rc82-AG2 | 1.1 | 1.1 | 1.1 | 1.7 | 2.1 | 1.1 | 1.5 | 1.8 | 2.3 | 3.2 | ||
| PSB Rc82-AG1-AG2 | 1.1 | 1.2 | 1.3 | 1.9 | 2.3 | 1.1 | 1.5 | 1.8 | 2.3 | 3.2 | ||
| Ciherang-Sub1-AG1 | 1.1 | 1.1 | 1.1 | 1.6 | 2.1 | 1.1 | 1.5 | 1.9 | 2.3 | 3.3 | ||
| Ciherang-Sub1-AG2 | 1.1 | 1.2 | 1.1 | 1.8 | 2.4 | 1.1 | 1.5 | 1.9 | 2.3 | 3.3 | ||
| Ciherang-Sub1-AG1-AG2 | 1.1 | 1.2 | 1.2 | 1.9 | 2.5 | 1.1 | 1.6 | 2.0 | 2.4 | 3.3 | ||
| PSB Rc82 | 0.9 | 0.9 | 0.9 | 0.9 | 1.0 | 0.9 | 1.7 | 1.9 | 2.6 | 3.2 | ||
| Ciherang-Sub1 | 1.0 | 1.0 | 1.0 | 1.0 | 1.4 | 1.0 | 1.6 | 2.0 | 2.5 | 3.3 | ||
| Kho Hlan On | 1.2 | 1.2 | 1.2 | 2.1 | 3.0 | 1.2 | 1.6 | 2.0 | 2.5 | 3.3 | ||
| Ma-Zhan Red | 1.2 | 1.2 | 1.2 | 1.9 | 2.7 | 1.2 | 1.5 | 2.0 | 2.5 | 3.3 | ||
| Mean | 1.1 | 1.1 | 1.1 | 1.6 | 2.2 | 1.1 | 1.6 | 1.9 | 2.4 | 3.3 | ||
| HSD0.05 | ns | 0.20*** | 0.19*** | 0.31*** | 0.37*** | ns | ns | ns | 0.31*** | ns | ||
| Starch | PSB Rc82-AG1 | 74.5 | 72.8 | 69.9 | 66.9 | 64.0 | 74.5 | 69.1 | 63.1 | 56.1 | 24.5 | |
| PSB Rc82-AG2 | 77.6 | 74.2 | 66.8 | 64.0 | 61.1 | 77.6 | 71.4 | 62.9 | 56.4 | 23.4 | ||
| PSB Rc82-AG1-AG2 | 75.8 | 72.9 | 70.3 | 66.5 | 60.2 | 75.8 | 69.9 | 64.6 | 54.7 | 22.4 | ||
| Ciherang-Sub1-AG1 | 75.3 | 72.9 | 69.4 | 66.6 | 62.0 | 75.3 | 72.3 | 65.6 | 57.8 | 25.3 | ||
| Ciherang-Sub1-AG2 | 77.5 | 75.0 | 71.2 | 66.0 | 60.6 | 77.5 | 69.6 | 63.7 | 55.4 | 16.5 | ||
| Ciherang-Sub1-AG1-AG2 | 76.7 | 73.8 | 69.1 | 65.7 | 59.8 | 76.7 | 71.3 | 63.9 | 58.0 | 17.8 | ||
| PSB Rc82 | 75.8 | 74.5 | 74.0 | 72.1 | 68.1 | 75.8 | 70.3 | 63.0 | 57.0 | 27.1 | ||
| Ciherang-Sub1 | 74.5 | 72.9 | 72.3 | 69.6 | 65.8 | 74.5 | 72.0 | 66.1 | 56.4 | 20.7 | ||
| Kho Hlan On | 78.9 | 73.2 | 70.2 | 65.4 | 59.0 | 78.9 | 72.6 | 66.5 | 55.5 | 17.1 | ||
| Ma-Zhan Red | 76.1 | 71.3 | 70.5 | 65.5 | 60.0 | 76.1 | 68.8 | 63.0 | 58.0 | 15.2 | ||
| Mean | 76.3 | 73.4 | 70.4 | 66.8 | 62.1 | 76.3 | 70.7 | 64.2 | 56.5 | 21.0 | ||
| HSD0.05 | ns | ns | ns | ns | 5.23*** | ns | ns | ns | ns | ns | ||
| Genotype | Soluble sugar concentration (%) | Starch concentration (%) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Flooded | Control | Flooded | Control | ||||||||||||
| 28 DAS | PI | MS | 28 DAS | PI | MS | 28 DAS | PI | MS | 28 DAS | PI | MS | ||||
| Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 2.6 | 5.4 | 6.3 | 3.9 | 6.0 | 5.7 | 3.5 | 5.5 | 3.5 | 3.8 | 6.1 | 3.0 | |||
| Ciherang-Sub1-AG2 | 2.9 | 8.1 | 5.5 | 3.9 | 7.9 | 5.0 | 3.9 | 7.4 | 3.4 | 4.1 | 6.9 | 1.8 | |||
| Ciherang-Sub1-AG1-AG2 | 2.5 | 9.1 | 7.1 | 5.5 | 8.4 | 5.4 | 3.5 | 6.8 | 2.9 | 4.2 | 6.6 | 2.7 | |||
| Ciherang-Sub1 | 1.9 | 5.7 | 5.0 | 4.4 | 6.3 | 4.4 | 3.7 | 4.4 | 2.9 | 3.8 | 5.8 | 2.2 | |||
| Kho Hlan On | 3.9 | 9.8 | 4.8 | 4.3 | 9.9 | 4.4 | 3.8 | 6.1 | 2.9 | 3.6 | 6.7 | 3.8 | |||
| Ma-Zhan Red | 4.0 | 8.5 | 4.7 | 3.8 | 8.5 | 4.7 | 3.6 | 6.0 | 3.0 | 2.9 | 6.7 | 2.7 | |||
| Mean | 3.0 | 7.8 | 5.6 | 4.3 | 7.8 | 4.9 | 3.7 | 6.0 | 3.1 | 3.7 | 6.5 | 2.7 | |||
| HSD0.05 | 0.97*** | ns | ns | 0.97*** | ns | ns | 0.92* | 0.84*** | ns | 0.92* | 0.84*** | ns | |||
| Dry season in 2017 | |||||||||||||||
| PSB Rc82-AG1 | 3.3 | 5.4 | 4.5 | 3.5 | 4.8 | 3.1 | 3.6 | 3.5 | 0.9 | 4.7 | 6.4 | 2.8 | |||
| PSB Rc82-AG2 | 4.0 | 5.0 | 3.8 | 2.6 | 4.2 | 3.8 | 3.8 | 3.9 | 1.3 | 5.9 | 7.2 | 2.1 | |||
| PSB Rc82-AG1-AG2 | 3.0 | 5.2 | 2.3 | 2.8 | 4.3 | 4.0 | 4.2 | 4.8 | 1.2 | 4.6 | 5.5 | 4.0 | |||
| Ciherang-Sub1-AG1 | 4.0 | 5.6 | 4.8 | 4.1 | 5.3 | 4.0 | 4.4 | 3.8 | 1.2 | 4.7 | 5.3 | 1.2 | |||
| Ciherang-Sub1-G2 | 4.7 | 7.0 | 4.9 | 4.5 | 7.2 | 5.6 | 4.7 | 6.4 | 1.6 | 4.1 | 5.4 | 2.4 | |||
| Ciherang-Sub1-AG1-AG2 | 5.4 | 5.4 | 3.3 | 4.2 | 5.8 | 4.0 | 4.1 | 5.7 | 1.5 | 5.1 | 5.2 | 1.9 | |||
| PSB Rc82 | 3.4 | 6.3 | 2.8 | 3.1 | 4.9 | 3.9 | 3.7 | 6.6 | 1.1 | 4.2 | 5.9 | 1.4 | |||
| Ciherang-Sub1 | 4.3 | 7.5 | 2.3 | 4.6 | 5.0 | 3.5 | 4.8 | 5.1 | 1.9 | 4.1 | 4.7 | 2.1 | |||
| Kho Hlan On | 4.6 | 8.8 | 4.3 | 6.0 | 8.4 | 5.6 | 7.4 | 8.5 | 2.6 | 4.0 | 7.4 | 3.4 | |||
| Ma-Zhan Red | 3.6 | 8.0 | 4.8 | 4.6 | 5.7 | 2.6 | 4.7 | 5.9 | 1.6 | 4.1 | 4.4 | 1.9 | |||
| Mean | 4.0 | 6.4 | 3.8 | 4.0 | 5.6 | 4.0 | 4.5 | 5.4 | 1.5 | 4.5 | 5.7 | 2.3 | |||
| HSD0.05 | ns | ns | 1.71*** | ns | ns | 1.71*** | 1.08*** | 1.76*** | 0.79*** | 1.08*** | 1.76*** | 0.79*** | |||
Table 2 Sugar and starch concentrations in rice stem at different growth stages under flooded with 3-5 cm water depth and control conditions in field. %
| Genotype | Soluble sugar concentration (%) | Starch concentration (%) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Flooded | Control | Flooded | Control | ||||||||||||
| 28 DAS | PI | MS | 28 DAS | PI | MS | 28 DAS | PI | MS | 28 DAS | PI | MS | ||||
| Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 2.6 | 5.4 | 6.3 | 3.9 | 6.0 | 5.7 | 3.5 | 5.5 | 3.5 | 3.8 | 6.1 | 3.0 | |||
| Ciherang-Sub1-AG2 | 2.9 | 8.1 | 5.5 | 3.9 | 7.9 | 5.0 | 3.9 | 7.4 | 3.4 | 4.1 | 6.9 | 1.8 | |||
| Ciherang-Sub1-AG1-AG2 | 2.5 | 9.1 | 7.1 | 5.5 | 8.4 | 5.4 | 3.5 | 6.8 | 2.9 | 4.2 | 6.6 | 2.7 | |||
| Ciherang-Sub1 | 1.9 | 5.7 | 5.0 | 4.4 | 6.3 | 4.4 | 3.7 | 4.4 | 2.9 | 3.8 | 5.8 | 2.2 | |||
| Kho Hlan On | 3.9 | 9.8 | 4.8 | 4.3 | 9.9 | 4.4 | 3.8 | 6.1 | 2.9 | 3.6 | 6.7 | 3.8 | |||
| Ma-Zhan Red | 4.0 | 8.5 | 4.7 | 3.8 | 8.5 | 4.7 | 3.6 | 6.0 | 3.0 | 2.9 | 6.7 | 2.7 | |||
| Mean | 3.0 | 7.8 | 5.6 | 4.3 | 7.8 | 4.9 | 3.7 | 6.0 | 3.1 | 3.7 | 6.5 | 2.7 | |||
| HSD0.05 | 0.97*** | ns | ns | 0.97*** | ns | ns | 0.92* | 0.84*** | ns | 0.92* | 0.84*** | ns | |||
| Dry season in 2017 | |||||||||||||||
| PSB Rc82-AG1 | 3.3 | 5.4 | 4.5 | 3.5 | 4.8 | 3.1 | 3.6 | 3.5 | 0.9 | 4.7 | 6.4 | 2.8 | |||
| PSB Rc82-AG2 | 4.0 | 5.0 | 3.8 | 2.6 | 4.2 | 3.8 | 3.8 | 3.9 | 1.3 | 5.9 | 7.2 | 2.1 | |||
| PSB Rc82-AG1-AG2 | 3.0 | 5.2 | 2.3 | 2.8 | 4.3 | 4.0 | 4.2 | 4.8 | 1.2 | 4.6 | 5.5 | 4.0 | |||
| Ciherang-Sub1-AG1 | 4.0 | 5.6 | 4.8 | 4.1 | 5.3 | 4.0 | 4.4 | 3.8 | 1.2 | 4.7 | 5.3 | 1.2 | |||
| Ciherang-Sub1-G2 | 4.7 | 7.0 | 4.9 | 4.5 | 7.2 | 5.6 | 4.7 | 6.4 | 1.6 | 4.1 | 5.4 | 2.4 | |||
| Ciherang-Sub1-AG1-AG2 | 5.4 | 5.4 | 3.3 | 4.2 | 5.8 | 4.0 | 4.1 | 5.7 | 1.5 | 5.1 | 5.2 | 1.9 | |||
| PSB Rc82 | 3.4 | 6.3 | 2.8 | 3.1 | 4.9 | 3.9 | 3.7 | 6.6 | 1.1 | 4.2 | 5.9 | 1.4 | |||
| Ciherang-Sub1 | 4.3 | 7.5 | 2.3 | 4.6 | 5.0 | 3.5 | 4.8 | 5.1 | 1.9 | 4.1 | 4.7 | 2.1 | |||
| Kho Hlan On | 4.6 | 8.8 | 4.3 | 6.0 | 8.4 | 5.6 | 7.4 | 8.5 | 2.6 | 4.0 | 7.4 | 3.4 | |||
| Ma-Zhan Red | 3.6 | 8.0 | 4.8 | 4.6 | 5.7 | 2.6 | 4.7 | 5.9 | 1.6 | 4.1 | 4.4 | 1.9 | |||
| Mean | 4.0 | 6.4 | 3.8 | 4.0 | 5.6 | 4.0 | 4.5 | 5.4 | 1.5 | 4.5 | 5.7 | 2.3 | |||
| HSD0.05 | ns | ns | 1.71*** | ns | ns | 1.71*** | 1.08*** | 1.76*** | 0.79*** | 1.08*** | 1.76*** | 0.79*** | |||
Fig. 1. Relationship of stem nonstructural carbohydrates (NSCs) with plant height at different growth stages under flooded condition.
| Genotypes | Amylose content (%) | Sugar content (%) | Starch content (%) |
|---|---|---|---|
| PSB Rc82-AG1 | 20.3±0.5 | 1.78±0.2 | 56.6±0.5 |
| PSB Rc82-AG2 | 20.8±1.4 | 2.04±0.1 | 58.4±0.6 |
| PSB Rc82-AG1-AG2 | 22.7±0.9 | 2.12±0.0 | 55.7±0.6 |
| Ciherang-Sub1-AG1 | 21.1±0.2 | 1.31±0.1 | 59.4±0.8 |
| Ciherang-Sub1-AG2 | 21.4±0.5 | 1.6±0.1 | 63.7±4.3 |
| Ciherang-Sub1-AG1-AG2 | 20.9±0.4 | 1.23±0.1 | 60.7±1.5 |
| PSB Rc82 | 22.1±0.8 | 1.91±0.1 | 57.7±1.5 |
| Ciherang-Sub1 | 21.0±0.6 | 1.49±0.2 | 67.4±7.9 |
Supplemental Table 1. Amylose, sugar and starch content were determined using paddy rice and expressed as percentage (weight basis).
| Genotypes | Amylose content (%) | Sugar content (%) | Starch content (%) |
|---|---|---|---|
| PSB Rc82-AG1 | 20.3±0.5 | 1.78±0.2 | 56.6±0.5 |
| PSB Rc82-AG2 | 20.8±1.4 | 2.04±0.1 | 58.4±0.6 |
| PSB Rc82-AG1-AG2 | 22.7±0.9 | 2.12±0.0 | 55.7±0.6 |
| Ciherang-Sub1-AG1 | 21.1±0.2 | 1.31±0.1 | 59.4±0.8 |
| Ciherang-Sub1-AG2 | 21.4±0.5 | 1.6±0.1 | 63.7±4.3 |
| Ciherang-Sub1-AG1-AG2 | 20.9±0.4 | 1.23±0.1 | 60.7±1.5 |
| PSB Rc82 | 22.1±0.8 | 1.91±0.1 | 57.7±1.5 |
| Ciherang-Sub1 | 21.0±0.6 | 1.49±0.2 | 67.4±7.9 |
Fig. 2. Effects of seeding rate and depth on survival of rice genotypes at 21 d after seedling.A, Under flooded condition of 3-5 cm water depth in wet season of 2016. B, Under control condition in wet season of 2016. C, Under flooded condition of 3-5 cm water depth in dry season of 2017. D, Under control condition in dry season of 2017.S1, S2 and S3, Seeding rates at 4, 8 and 12 g/m2, respectively; D1 and D2, Depth of seeding with 0.5 and 2.0 cm, respectively. L1, Ciherang-Sub1-AG1; L2, Ciherang-Sub1-AG2; L3, Ciherang-Sub1-AG1-AG2; L4, Ciherang-Sub1; L5, Ma-Zhan Red.Data indicate Mean ± SE (n = 3).
Fig. 3. Effects of seeding rate and depth on germination rate of rice genotypes under the flooded condition.A-E, Wet season in 2016. F-J, Dry season in 2017. S1, S2 and S3, Seeding rates at 4, 8 and 12 g/m2, respectively; D1 and D2, Depths of seeding with 0.5 and 2.0 cm, respectively. ns, No significance; Vertical bars indicate significant difference at the LSD0.05 level (n = 3).
Fig. 4. Effect of seeding rate and depth on rice seedling vigor index at 28 d after seedling.A, Under flooded condition of 3-5 cm water depth in wet season 2016. B, Under control condition in wet season 2016. C, Under flooded condition of 3-5 cm water depth in dry season of 2017. D, Under control condition in dry season of 2017.L1, Ciherang-Sub1-AG1; L2, Ciherang-Sub1-AG2; L3, Ciherang-Sub1-AG1-AG2; L4, Ciherang-Sub1; L5, Ma-Zhan Red. S1, S2 and S3, Seeding rate at 4, 8 and 12 g/m2, respectively; D1 and D2, Depth of seeding with 0.5 cm and 2.0 cm, respectively. Data are Mean ± SE (n = 3).
| Genotype | Control | Flooded | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D1 | D2 | ||||||||||||
| S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | ||||
| Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 9.2 | 11.8 | 8.7 | 11.2 | 12.5 | 12.7 | 4.2 | 2.3 | 3.4 | 4.1 | 5.8 | 3.8 | |||
| Ciherang-Sub1-AG2 | 9.6 | 10.9 | 10.4 | 11.2 | 10.2 | 10.6 | 4.7 | 4.7 | 3.5 | 4.0 | 6.6 | 8.4 | |||
| Ciherang-Sub1-AG1-AG2 | 9.6 | 9.9 | 12.0 | 13.0 | 11.1 | 13.9 | 3.8 | 3.5 | 2.9 | 4.9 | 6.5 | 6.2 | |||
| Ciherang-Sub1 | 10.0 | 11.0 | 10.5 | 13.6 | 12.3 | 15.2 | 2.6 | 3.7 | 2.7 | 2.5 | 4.4 | 4.4 | |||
| Ma-Zhan Red | 12.4 | 11.7 | 13.5 | 10.9 | 12.4 | 15.9 | 3.8 | 4.1 | 6.7 | 5.6 | 4.8 | 4.7 | |||
| Mean | 10.2 | 11.1 | 11.0 | 12.0 | 11.7 | 13.7 | 3.8 | 3.7 | 3.9 | 4.2 | 5.6 | 5.5 | |||
| HSD0.05 | ns | ns | ns | ns | ns | ns | 1.0** | 1.0** | 1.0** | 1.0** | 1.0** | 1.0** | |||
| Dry season in 2017 | |||||||||||||||
| Ciherang-Sub1-AG1 | 10.0 | 9.5 | 9.5 | 12.3 | 9.5 | 11.0 | 9.0 | 5.7 | 10.6 | 10.9 | 10.0 | 10.6 | |||
| Ciherang-Sub1-AG2 | 13.8 | 10.5 | 15.4 | 8.6 | 13.8 | 11.0 | 7.5 | 6.9 | 13.2 | 11.8 | 11.4 | 10.0 | |||
| Ciherang-Sub1-AG1-AG2 | 12.3 | 9.3 | 11.8 | 10.2 | 12.2 | 14.7 | 9.7 | 9.8 | 12.5 | 13.7 | 11.8 | 9.7 | |||
| Ciherang-Sub1 | 8.4 | 7.9 | 9.7 | 9.9 | 10.1 | 11.3 | 8.7 | 6.7 | 11.2 | 10.5 | 9.3 | 8.5 | |||
| Ma-Zhan Red | 9.2 | 10.8 | 10.4 | 11.9 | 13.1 | 12.2 | 7.9 | 7.3 | 9.8 | 10.7 | 9.6 | 8.7 | |||
| Mean | 10.7 | 9.6 | 11.3 | 10.6 | 11.7 | 12.0 | 8.6 | 7.3 | 11.4 | 11.5 | 10.4 | 9.5 | |||
| HSD0.05 | ns | ns | ns | ns | ns | ns | 0.84* | 0.84* | 0.84* | 0.84* | 0.84* | 0.84* | |||
Table 3 Effects of seeding rate and depth on specific leaf area of different AG-introgression lines at 21 d after sowing. cm2/g
| Genotype | Control | Flooded | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D1 | D2 | ||||||||||||
| S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | ||||
| Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 9.2 | 11.8 | 8.7 | 11.2 | 12.5 | 12.7 | 4.2 | 2.3 | 3.4 | 4.1 | 5.8 | 3.8 | |||
| Ciherang-Sub1-AG2 | 9.6 | 10.9 | 10.4 | 11.2 | 10.2 | 10.6 | 4.7 | 4.7 | 3.5 | 4.0 | 6.6 | 8.4 | |||
| Ciherang-Sub1-AG1-AG2 | 9.6 | 9.9 | 12.0 | 13.0 | 11.1 | 13.9 | 3.8 | 3.5 | 2.9 | 4.9 | 6.5 | 6.2 | |||
| Ciherang-Sub1 | 10.0 | 11.0 | 10.5 | 13.6 | 12.3 | 15.2 | 2.6 | 3.7 | 2.7 | 2.5 | 4.4 | 4.4 | |||
| Ma-Zhan Red | 12.4 | 11.7 | 13.5 | 10.9 | 12.4 | 15.9 | 3.8 | 4.1 | 6.7 | 5.6 | 4.8 | 4.7 | |||
| Mean | 10.2 | 11.1 | 11.0 | 12.0 | 11.7 | 13.7 | 3.8 | 3.7 | 3.9 | 4.2 | 5.6 | 5.5 | |||
| HSD0.05 | ns | ns | ns | ns | ns | ns | 1.0** | 1.0** | 1.0** | 1.0** | 1.0** | 1.0** | |||
| Dry season in 2017 | |||||||||||||||
| Ciherang-Sub1-AG1 | 10.0 | 9.5 | 9.5 | 12.3 | 9.5 | 11.0 | 9.0 | 5.7 | 10.6 | 10.9 | 10.0 | 10.6 | |||
| Ciherang-Sub1-AG2 | 13.8 | 10.5 | 15.4 | 8.6 | 13.8 | 11.0 | 7.5 | 6.9 | 13.2 | 11.8 | 11.4 | 10.0 | |||
| Ciherang-Sub1-AG1-AG2 | 12.3 | 9.3 | 11.8 | 10.2 | 12.2 | 14.7 | 9.7 | 9.8 | 12.5 | 13.7 | 11.8 | 9.7 | |||
| Ciherang-Sub1 | 8.4 | 7.9 | 9.7 | 9.9 | 10.1 | 11.3 | 8.7 | 6.7 | 11.2 | 10.5 | 9.3 | 8.5 | |||
| Ma-Zhan Red | 9.2 | 10.8 | 10.4 | 11.9 | 13.1 | 12.2 | 7.9 | 7.3 | 9.8 | 10.7 | 9.6 | 8.7 | |||
| Mean | 10.7 | 9.6 | 11.3 | 10.6 | 11.7 | 12.0 | 8.6 | 7.3 | 11.4 | 11.5 | 10.4 | 9.5 | |||
| HSD0.05 | ns | ns | ns | ns | ns | ns | 0.84* | 0.84* | 0.84* | 0.84* | 0.84* | 0.84* | |||
| Genotype | Saturated | |||||
|---|---|---|---|---|---|---|
| D1 | D2 | |||||
| Wet season-2016 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 |
| Ciherang-Sub1-AG1 | 4.4 | 5.8 | 4.2 | 5.2667 | 4.8 | 6.5 |
| Ciherang-Sub1-AG2 | 4.4 | 5.1 | 4.7 | 5.0767 | 4.7 | 5.9 |
| Ciherang-Sub1-AG1-AG2 | 4.1 | 5.0 | 4.8 | 6.08 | 5.5 | 6.0 |
| Ciherang-Sub1 | 4.8 | 4.0 | 3.1 | 4.0833 | 4.2 | 6.7 |
| Ma-Zhan Red | 4.7 | 4.9 | 6.3 | 9.4267 | 6.9 | 8.1 |
| Mean | 4.5 | 5.0 | 4.6 | 6.0 | 5.2 | 6.6 |
| LSD 0.05 | 0.93** | 0.93** | 0.93** | 0.93** | 0.93** | 0.93** |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 1.2 | 1.8 | 1.6 | 1.9 | 1.9 | 1.5 |
| Ciherang-Sub1-AG2 | 2.3 | 2.5 | 1.9 | 1.7 | 1.9 | 2.0 |
| Ciherang-Sub1-AG1-AG2 | 2.0 | 2.3 | 1.9 | 1.4 | 1.5 | 1.6 |
| Ciherang-Sub1 | 1.3 | 1.3 | 1.5 | 1.1 | 1.0 | 1.2 |
| Ma-Zhan Red | 1.9 | 1.8 | 2.0 | 2.3 | 1.5 | 1.2 |
| Mean | 1.8 | 1.9 | 1.8 | 1.7 | 1.6 | 1.5 |
| LSD 0.05 | 0.49* | 0.49* | 0.49* | 0.49* | 0.49* | 0.49* |
| Dry season 2017 | Saturated | |||||
| Ciherang-Sub1-AG1 | 4.6 | 3.9 | 4.9 | 3.9 | 4.1 | 3.7 |
| Ciherang-Sub1-AG2 | 4.6 | 3.9 | 5.2 | 4.4 | 4.9 | 4.4 |
| Ciherang-Sub1-AG1-AG2 | 4.7 | 3.3 | 6.0 | 5.2 | 6.1 | 4.7 |
| Ciherang-Sub1 | 5.4 | 2.4 | 3.6 | 3.0 | 4.0 | 3.7 |
| Ma-Zhan Red | 5.2 | 4.3 | 5.9 | 4.4 | 5.4 | 5.5 |
| Mean | 4.9 | 3.5 | 5.1 | 4.2 | 4.9 | 4.4 |
| LSD 0.05 | ns | ns | ns | ns | ns | ns |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 2.2 | 1.2 | 2.2 | 3.5 | 2.4 | 2.3 |
| Ciherang-Sub1-AG2 | 3.0 | 2.5 | 2.2 | 2.7 | 2.9 | 2.6 |
| Ciherang-Sub1-AG1-AG2 | 3.4 | 2.9 | 2.6 | 2.7 | 3.2 | 2.7 |
| Ciherang-Sub1 | 2.1 | 1.7 | 2.0 | 1.8 | 2.4 | 2.0 |
| Ma-Zhan Red | 2.3 | 2.5 | 2.2 | 2.2 | 2.3 | 2.3 |
| Mean | 2.6 | 2.1 | 2.3 | 2.6 | 2.6 | 2.4 |
| LSD 0.05 | 0.52 | 0.52* | 0.52* | 0.52* | 0.52* | 0.52* |
Supplemental Table 2. Effect of seeding rate and depth on leaf area per plant (cm-2) of different AG-introgression lines at 14 DAS during the wet season of 2016 and dry season 2017.
| Genotype | Saturated | |||||
|---|---|---|---|---|---|---|
| D1 | D2 | |||||
| Wet season-2016 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 |
| Ciherang-Sub1-AG1 | 4.4 | 5.8 | 4.2 | 5.2667 | 4.8 | 6.5 |
| Ciherang-Sub1-AG2 | 4.4 | 5.1 | 4.7 | 5.0767 | 4.7 | 5.9 |
| Ciherang-Sub1-AG1-AG2 | 4.1 | 5.0 | 4.8 | 6.08 | 5.5 | 6.0 |
| Ciherang-Sub1 | 4.8 | 4.0 | 3.1 | 4.0833 | 4.2 | 6.7 |
| Ma-Zhan Red | 4.7 | 4.9 | 6.3 | 9.4267 | 6.9 | 8.1 |
| Mean | 4.5 | 5.0 | 4.6 | 6.0 | 5.2 | 6.6 |
| LSD 0.05 | 0.93** | 0.93** | 0.93** | 0.93** | 0.93** | 0.93** |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 1.2 | 1.8 | 1.6 | 1.9 | 1.9 | 1.5 |
| Ciherang-Sub1-AG2 | 2.3 | 2.5 | 1.9 | 1.7 | 1.9 | 2.0 |
| Ciherang-Sub1-AG1-AG2 | 2.0 | 2.3 | 1.9 | 1.4 | 1.5 | 1.6 |
| Ciherang-Sub1 | 1.3 | 1.3 | 1.5 | 1.1 | 1.0 | 1.2 |
| Ma-Zhan Red | 1.9 | 1.8 | 2.0 | 2.3 | 1.5 | 1.2 |
| Mean | 1.8 | 1.9 | 1.8 | 1.7 | 1.6 | 1.5 |
| LSD 0.05 | 0.49* | 0.49* | 0.49* | 0.49* | 0.49* | 0.49* |
| Dry season 2017 | Saturated | |||||
| Ciherang-Sub1-AG1 | 4.6 | 3.9 | 4.9 | 3.9 | 4.1 | 3.7 |
| Ciherang-Sub1-AG2 | 4.6 | 3.9 | 5.2 | 4.4 | 4.9 | 4.4 |
| Ciherang-Sub1-AG1-AG2 | 4.7 | 3.3 | 6.0 | 5.2 | 6.1 | 4.7 |
| Ciherang-Sub1 | 5.4 | 2.4 | 3.6 | 3.0 | 4.0 | 3.7 |
| Ma-Zhan Red | 5.2 | 4.3 | 5.9 | 4.4 | 5.4 | 5.5 |
| Mean | 4.9 | 3.5 | 5.1 | 4.2 | 4.9 | 4.4 |
| LSD 0.05 | ns | ns | ns | ns | ns | ns |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 2.2 | 1.2 | 2.2 | 3.5 | 2.4 | 2.3 |
| Ciherang-Sub1-AG2 | 3.0 | 2.5 | 2.2 | 2.7 | 2.9 | 2.6 |
| Ciherang-Sub1-AG1-AG2 | 3.4 | 2.9 | 2.6 | 2.7 | 3.2 | 2.7 |
| Ciherang-Sub1 | 2.1 | 1.7 | 2.0 | 1.8 | 2.4 | 2.0 |
| Ma-Zhan Red | 2.3 | 2.5 | 2.2 | 2.2 | 2.3 | 2.3 |
| Mean | 2.6 | 2.1 | 2.3 | 2.6 | 2.6 | 2.4 |
| LSD 0.05 | 0.52 | 0.52* | 0.52* | 0.52* | 0.52* | 0.52* |
| Genotype | Saturated | |||||
|---|---|---|---|---|---|---|
| D1 | D2 | |||||
| Wet season 2016 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 |
| Ciherang-Sub1-AG1 | 303.4 | 988 | 1206.6 | 437 | 577.4 | 1181.2 |
| Ciherang-Sub1-AG2 | 420.5 | 972.8 | 1016.1 | 446.7 | 614.4 | 1288 |
| Ciherang-Sub1-AG1-AG2 | 379.3 | 730.4 | 1030 | 461 | 731.1 | 1058.3 |
| Ciherang-Sub1 | 400.5 | 761.3 | 1076 | 421.4 | 635.7 | 1290.6 |
| Ma-Zhan Red | 521.7 | 1139.4 | 1341.5 | 594.4 | 1038.5 | 1710 |
| Mean | 405.1 | 918.4 | 1134 | 472.1 | 719.4 | 1305.6 |
| LSD 0.05 | ns | ns | ns | ns | ns | ns |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 45.6 | 82.7 | 139.7 | 52.6 | 95.5 | 159.4 |
| Ciherang-Sub1-AG2 | 76.6 | 117.6 | 168.1 | 64.3 | 115.8 | 244.9 |
| Ciherang-Sub1-AG1-AG2 | 66.3 | 82.6 | 135.6 | 53.9 | 112.4 | 201 |
| Ciherang-Sub1 | 18.5 | 39.8 | 60.8 | 25.2 | 77.2 | 74.3 |
| Ma-Zhan Red | 65.2 | 153.4 | 297.6 | 141.4 | 202.8 | 284.2 |
| Mean | 54.4 | 95.2 | 160.4 | 67.5 | 120.7 | 192.8 |
| LSD 0.05 | 39.1** | 39.1** | 39.1** | 39.1** | 39.1** | 39.1** |
| Dry season 2017 | Saturated | |||||
| Ciherang-Sub1-AG1 | 378.4 | 861.9 | 1305.8 | 500.4 | 766.3 | 1167.5 |
| Ciherang-Sub1-AG2 | 461.6 | 859.2 | 1317.8 | 413.5 | 961.4 | 1315.4 |
| Ciherang-Sub1-AG1-AG2 | 491.4 | 679.6 | 1210.6 | 492.9 | 884.7 | 1037.9 |
| Ciherang-Sub1 | 553.1 | 843.6 | 1272.9 | 407.7 | 768.4 | 1187.7 |
| Ma-Zhan Red | 569.7 | 1014.7 | 1376.6 | 506.8 | 953.6 | 1761.3 |
| Mean | 490.8 | 851.8 | 1296.7 | 464.3 | 866.9 | 1294 |
| LSD 0.05 | ns | ns | ns | ns | ns | ns |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 260.9 | 292.8 | 716 | 212 | 367.4 | 354 |
| Ciherang-Sub1-AG2 | 241.6 | 420 | 389.6 | 232.9 | 536.2 | 363 |
| Ciherang-Sub1-AG1-AG2 | 302.4 | 607.1 | 602.1 | 312 | 527.7 | 548 |
| Ciherang-Sub1 | 152 | 175.7 | 249.4 | 134.1 | 169.3 | 195.7 |
| Ma-Zhan Red | 314.3 | 670 | 704.2 | 268.4 | 572.4 | 787.7 |
| Mean | 254.2 | 433.1 | 532.3 | 231.9 | 434.6 | 449.7 |
| LSD 0.05 | 121.4* | 121.4* | 121.4* | 121.4* | 121.4* | 121.4* |
Supplemental Table 3. Effect of seeding rate and depth on leaf no per m2 of different AG introgression lines at 14 DAS during the wet season 2016 and dry season 2017.
| Genotype | Saturated | |||||
|---|---|---|---|---|---|---|
| D1 | D2 | |||||
| Wet season 2016 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 | 4 g/m2 | 8 g/m2 |
| Ciherang-Sub1-AG1 | 303.4 | 988 | 1206.6 | 437 | 577.4 | 1181.2 |
| Ciherang-Sub1-AG2 | 420.5 | 972.8 | 1016.1 | 446.7 | 614.4 | 1288 |
| Ciherang-Sub1-AG1-AG2 | 379.3 | 730.4 | 1030 | 461 | 731.1 | 1058.3 |
| Ciherang-Sub1 | 400.5 | 761.3 | 1076 | 421.4 | 635.7 | 1290.6 |
| Ma-Zhan Red | 521.7 | 1139.4 | 1341.5 | 594.4 | 1038.5 | 1710 |
| Mean | 405.1 | 918.4 | 1134 | 472.1 | 719.4 | 1305.6 |
| LSD 0.05 | ns | ns | ns | ns | ns | ns |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 45.6 | 82.7 | 139.7 | 52.6 | 95.5 | 159.4 |
| Ciherang-Sub1-AG2 | 76.6 | 117.6 | 168.1 | 64.3 | 115.8 | 244.9 |
| Ciherang-Sub1-AG1-AG2 | 66.3 | 82.6 | 135.6 | 53.9 | 112.4 | 201 |
| Ciherang-Sub1 | 18.5 | 39.8 | 60.8 | 25.2 | 77.2 | 74.3 |
| Ma-Zhan Red | 65.2 | 153.4 | 297.6 | 141.4 | 202.8 | 284.2 |
| Mean | 54.4 | 95.2 | 160.4 | 67.5 | 120.7 | 192.8 |
| LSD 0.05 | 39.1** | 39.1** | 39.1** | 39.1** | 39.1** | 39.1** |
| Dry season 2017 | Saturated | |||||
| Ciherang-Sub1-AG1 | 378.4 | 861.9 | 1305.8 | 500.4 | 766.3 | 1167.5 |
| Ciherang-Sub1-AG2 | 461.6 | 859.2 | 1317.8 | 413.5 | 961.4 | 1315.4 |
| Ciherang-Sub1-AG1-AG2 | 491.4 | 679.6 | 1210.6 | 492.9 | 884.7 | 1037.9 |
| Ciherang-Sub1 | 553.1 | 843.6 | 1272.9 | 407.7 | 768.4 | 1187.7 |
| Ma-Zhan Red | 569.7 | 1014.7 | 1376.6 | 506.8 | 953.6 | 1761.3 |
| Mean | 490.8 | 851.8 | 1296.7 | 464.3 | 866.9 | 1294 |
| LSD 0.05 | ns | ns | ns | ns | ns | ns |
| Flood | ||||||
| Ciherang-Sub1-AG1 | 260.9 | 292.8 | 716 | 212 | 367.4 | 354 |
| Ciherang-Sub1-AG2 | 241.6 | 420 | 389.6 | 232.9 | 536.2 | 363 |
| Ciherang-Sub1-AG1-AG2 | 302.4 | 607.1 | 602.1 | 312 | 527.7 | 548 |
| Ciherang-Sub1 | 152 | 175.7 | 249.4 | 134.1 | 169.3 | 195.7 |
| Ma-Zhan Red | 314.3 | 670 | 704.2 | 268.4 | 572.4 | 787.7 |
| Mean | 254.2 | 433.1 | 532.3 | 231.9 | 434.6 | 449.7 |
| LSD 0.05 | 121.4* | 121.4* | 121.4* | 121.4* | 121.4* | 121.4* |
| Genotype | Control | Flooded | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D1 | D2 | ||||||||||||
| S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | ||||
| Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 465.0 | 1299.5 | 1657.9 | 744.2 | 903.0 | 1743.3 | 67.2 | 123.2 | 206.9 | 76.9 | 126.3 | 246.3 | |||
| Ciherang-Sub1-AG2 | 580.9 | 1482.3 | 1736.5 | 700.0 | 1201.7 | 1937.6 | 133.4 | 138.0 | 284.1 | 90.5 | 264.2 | 482.2 | |||
| Ciherang-Sub1-AG1-AG2 | 578.4 | 1129.1 | 1512.1 | 618.2 | 1137.4 | 1840.1 | 66.1 | 103.6 | 153.8 | 65.5 | 175.9 | 298.7 | |||
| Ciherang-Sub1 | 494.8 | 1012.9 | 1525.5 | 654.9 | 1082.7 | 1555.0 | 16.5 | 51.9 | 101.1 | 18.9 | 96.2 | 113.7 | |||
| Ma-Zhan Red | 842.4 | 1545.6 | 1808.9 | 893.8 | 1538.9 | 2041.5 | 137.1 | 230.5 | 632.3 | 258.9 | 358.2 | 469.8 | |||
| Mean | 592.3 | 1293.9 | 1648.2 | 722.2 | 1172.7 | 1823.5 | 84.0 | 129.4 | 275.7 | 102.2 | 204.2 | 322.1 | |||
| HSD0.05 | 222.5* | 222.5* | 222.5* | 222.5* | 222.5* | 222.5* | 81.3** | 81.3** | 81.3** | 81.3** | 81.3** | 81.3** | |||
| Dry season in 2017 | |||||||||||||||
| Ciherang-Sub1-AG1 | 629.0 | 1610.2 | 1978.1 | 1032.6 | 1212.7 | 2158.3 | 478.1 | 549.0 | 1779.3 | 553.2 | 742.1 | 1024.7 | |||
| Ciherang-Sub1-AG2 | 1026.7 | 1759.7 | 2678.3 | 835.9 | 1803.6 | 2304.6 | 486.6 | 813.8 | 1266.6 | 668.7 | 1144.6 | 894.4 | |||
| Ciherang-Sub1-AG1-AG2 | 954.0 | 1555.4 | 2020.9 | 864.8 | 1470.8 | 2086.3 | 590.8 | 1267.6 | 1632.0 | 942.9 | 1282.9 | 1357.1 | |||
| Ciherang-Sub1 | 799.4 | 1556.6 | 2145.7 | 860.3 | 1394.3 | 2018.6 | 305.6 | 412.2 | 725.1 | 335.0 | 386.6 | 447.0 | |||
| Ma-Zhan Red | 1043.3 | 1786.8 | 2358.5 | 1030.4 | 1902.6 | 2358.1 | 509.5 | 1323.4 | 1693.0 | 683.8 | 1052.7 | 1447.8 | |||
| Mean | 890.5 | 1653.7 | 2236.3 | 924.8 | 1556.8 | 2185.2 | 474.1 | 873.2 | 1419.2 | 636.7 | 921.8 | 1034.2 | |||
| HSD0.05 | ns | ns | ns | ns | ns | ns | 241.4* | 241.4* | 241.4* | 241.4* | 241.4* | 241.4* | |||
Table 4 Effects of seeding rate and depth on leaf number per square of different AG-introgression lines at 21 d after sowing.
| Genotype | Control | Flooded | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D1 | D2 | ||||||||||||
| S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | ||||
| Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 465.0 | 1299.5 | 1657.9 | 744.2 | 903.0 | 1743.3 | 67.2 | 123.2 | 206.9 | 76.9 | 126.3 | 246.3 | |||
| Ciherang-Sub1-AG2 | 580.9 | 1482.3 | 1736.5 | 700.0 | 1201.7 | 1937.6 | 133.4 | 138.0 | 284.1 | 90.5 | 264.2 | 482.2 | |||
| Ciherang-Sub1-AG1-AG2 | 578.4 | 1129.1 | 1512.1 | 618.2 | 1137.4 | 1840.1 | 66.1 | 103.6 | 153.8 | 65.5 | 175.9 | 298.7 | |||
| Ciherang-Sub1 | 494.8 | 1012.9 | 1525.5 | 654.9 | 1082.7 | 1555.0 | 16.5 | 51.9 | 101.1 | 18.9 | 96.2 | 113.7 | |||
| Ma-Zhan Red | 842.4 | 1545.6 | 1808.9 | 893.8 | 1538.9 | 2041.5 | 137.1 | 230.5 | 632.3 | 258.9 | 358.2 | 469.8 | |||
| Mean | 592.3 | 1293.9 | 1648.2 | 722.2 | 1172.7 | 1823.5 | 84.0 | 129.4 | 275.7 | 102.2 | 204.2 | 322.1 | |||
| HSD0.05 | 222.5* | 222.5* | 222.5* | 222.5* | 222.5* | 222.5* | 81.3** | 81.3** | 81.3** | 81.3** | 81.3** | 81.3** | |||
| Dry season in 2017 | |||||||||||||||
| Ciherang-Sub1-AG1 | 629.0 | 1610.2 | 1978.1 | 1032.6 | 1212.7 | 2158.3 | 478.1 | 549.0 | 1779.3 | 553.2 | 742.1 | 1024.7 | |||
| Ciherang-Sub1-AG2 | 1026.7 | 1759.7 | 2678.3 | 835.9 | 1803.6 | 2304.6 | 486.6 | 813.8 | 1266.6 | 668.7 | 1144.6 | 894.4 | |||
| Ciherang-Sub1-AG1-AG2 | 954.0 | 1555.4 | 2020.9 | 864.8 | 1470.8 | 2086.3 | 590.8 | 1267.6 | 1632.0 | 942.9 | 1282.9 | 1357.1 | |||
| Ciherang-Sub1 | 799.4 | 1556.6 | 2145.7 | 860.3 | 1394.3 | 2018.6 | 305.6 | 412.2 | 725.1 | 335.0 | 386.6 | 447.0 | |||
| Ma-Zhan Red | 1043.3 | 1786.8 | 2358.5 | 1030.4 | 1902.6 | 2358.1 | 509.5 | 1323.4 | 1693.0 | 683.8 | 1052.7 | 1447.8 | |||
| Mean | 890.5 | 1653.7 | 2236.3 | 924.8 | 1556.8 | 2185.2 | 474.1 | 873.2 | 1419.2 | 636.7 | 921.8 | 1034.2 | |||
| HSD0.05 | ns | ns | ns | ns | ns | ns | 241.4* | 241.4* | 241.4* | 241.4* | 241.4* | 241.4* | |||
Supplemental Fig. 1. Leaf area ratio (LAR) of rice seedling with different seeding rate and depth at 21 days after sowing under control and flooded condition during wet season 2016 and dry season 2017. S1, S2 and S3, Seeding rate at 4, 8 and 12 g/m2, respectively; D1 and D2, Depth of seeding with 0.5 cm and 2.0 cm, respectively. Vertical bars indicate Mean ± SE (n = 3).
Supplemental Fig. 2. Specific leaf area (SLA) of rice seedling with different seeding rate and depth at 21 days after sowing under control and flooded condition during wet season 2016 and dry season 2017.S1, S2 and S3, Seeding rate at 4, 8 and 12 g/m2, respectively; D1 and D2, Depth of seeding with 0.5 cm and 2.0 cm, respectively. Vertical bars indicate Mean ± SE (n = 3).
| Trait | Genotype | Control | Flooded | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D1 | D2 | |||||||||||||
| S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | |||||
| Leaf dry weight | Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 4.7 | 11.8 | 14.2 | 6.8 | 12.1 | 18.2 | 0.5 | 0.6 | 0.7 | 0.5 | 0.9 | 2.1 | ||||
| Ciherang-Sub1-AG2 | 5.6 | 15.4 | 15.5 | 5.0 | 10.1 | 20.6 | 0.9 | 0.9 | 1.8 | 1.3 | 1.6 | 2.3 | ||||
| Ciherang-Sub1-AG1-AG2 | 5.2 | 11.6 | 13.8 | 6.5 | 12.8 | 14.8 | 0.4 | 0.6 | 1.2 | 0.5 | 1.0 | 2.2 | ||||
| Ciherang-Sub1 | 3.3 | 12.7 | 15.4 | 7.4 | 10.4 | 16.2 | 0.1 | 0.3 | 0.3 | 0.2 | 0.8 | 0.6 | ||||
| Ma-Zhan Red | 7.9 | 16.0 | 15.5 | 12.4 | 20.1 | 29.4 | 0.8 | 1.4 | 4.1 | 1.6 | 3.8 | 3.9 | ||||
| Mean | 5.4 | 13.5 | 14.9 | 7.6 | 13.1 | 19.8 | 0.5 | 0.8 | 1.6 | 0.8 | 1.6 | 2.2 | ||||
| HSD0.05 | 3.53** | 0.46*** | ||||||||||||||
| Dry season in 2017 | ||||||||||||||||
| Ciherang-Sub1-AG1 | 5.0 | 11.9 | 15.9 | 8.2 | 9.8 | 17.3 | 3.9 | 3.6 | 13.4 | 4.0 | 5.6 | 7.5 | ||||
| Ciherang-Sub1-AG2 | 8.5 | 12.8 | 23.3 | 6.2 | 15.5 | 19.7 | 3.4 | 5.1 | 9.6 | 5.9 | 8.8 | 6.6 | ||||
| Ciherang-Sub1-AG1-AG2 | 8.0 | 11.4 | 16.3 | 7.1 | 12.4 | 18.9 | 5.1 | 8.2 | 12.6 | 7.8 | 10.5 | 10.7 | ||||
| Ciherang-Sub1 | 6.1 | 10.3 | 17.0 | 6.7 | 10.5 | 17.9 | 1.9 | 2.1 | 4.5 | 1.7 | 2.5 | 2.9 | ||||
| Ma-Zhan Red | 8.6 | 15.0 | 22.5 | 10.0 | 18.4 | 24.2 | 4.2 | 11.7 | 14.8 | 6.4 | 11.1 | 13.5 | ||||
| Mean | 7.2 | 12.3 | 19.0 | 7.6 | 13.3 | 19.6 | 3.7 | 6.1 | 11.0 | 5.1 | 7.7 | 8.2 | ||||
| HSD0.05 | ns | 2.24* | ||||||||||||||
| Stem dry weight | Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 3.9 | 10.2 | 12.0 | 5.8 | 12.0 | 16.8 | 0.3 | 0.8 | 1.4 | 0.6 | 1.0 | 2.3 | ||||
| Ciherang-Sub1-AG2 | 4.1 | 11.1 | 11.6 | 5.6 | 11.2 | 20.9 | 0.6 | 1.3 | 2.1 | 0.9 | 1.5 | 2.7 | ||||
| Ciherang-Sub1-AG1-AG2 | 5.1 | 10.0 | 14.2 | 5.5 | 10.6 | 15.2 | 0.3 | 0.8 | 2.0 | 0.7 | 1.5 | 2.5 | ||||
| Ciherang-Sub1 | 3.3 | 10.9 | 11.3 | 5.5 | 9.6 | 14.3 | 0.1 | 0.3 | 0.6 | 0.2 | 0.7 | 0.6 | ||||
| Ma-Zhan Red | 8.3 | 16.1 | 18.2 | 11.1 | 16.0 | 21.8 | 0.7 | 1.6 | 4.4 | 2.1 | 2.7 | 3.5 | ||||
| Mean | 4.9 | 11.7 | 13.5 | 6.7 | 11.9 | 17.8 | 0.4 | 1.0 | 2.1 | 0.9 | 1.5 | 2.3 | ||||
| HSD0.05 | ns | 0.47* | ||||||||||||||
| Dry season in 2017 | ||||||||||||||||
| Ciherang-Sub1-AG1 | 4.5 | 11.5 | 15.3 | 7.7 | 8.9 | 15.6 | 3.5 | 4.7 | 13.0 | 3.9 | 5.4 | 7.1 | ||||
| Ciherang-Sub1-AG2 | 7.2 | 12.9 | 21.9 | 5.7 | 14.7 | 18.6 | 3.2 | 6.3 | 9.2 | 5.5 | 8.3 | 5.7 | ||||
| Ciherang-Sub1-AG1-AG2 | 7.2 | 10.7 | 14.2 | 6.3 | 12.1 | 17.4 | 4.8 | 8.8 | 13.1 | 7.8 | 10.2 | 10.7 | ||||
| Ciherang-Sub1 | 6.2 | 9.5 | 16.4 | 6.1 | 10.0 | 17.0 | 1.6 | 2.2 | 4.5 | 1.6 | 2.6 | 2.4 | ||||
| Ma-Zhan Red | 7.4 | 12.3 | 19.2 | 8.7 | 16.1 | 22.2 | 3.7 | 9.8 | 13.9 | 5.8 | 9.3 | 12.1 | ||||
| Mean | 6.5 | 11.4 | 17.4 | 6.9 | 12.4 | 18.2 | 3.4 | 6.4 | 10.8 | 4.9 | 7.2 | 7.6 | ||||
| HSD0.05 | ns | ns | ||||||||||||||
Table 5 Effects of seeding rate and depth on leaf and stem dry weight at 21 d after sowing under control and flooded conditions. g/m2
| Trait | Genotype | Control | Flooded | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D1 | D2 | |||||||||||||
| S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | S1 | S2 | S3 | |||||
| Leaf dry weight | Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 4.7 | 11.8 | 14.2 | 6.8 | 12.1 | 18.2 | 0.5 | 0.6 | 0.7 | 0.5 | 0.9 | 2.1 | ||||
| Ciherang-Sub1-AG2 | 5.6 | 15.4 | 15.5 | 5.0 | 10.1 | 20.6 | 0.9 | 0.9 | 1.8 | 1.3 | 1.6 | 2.3 | ||||
| Ciherang-Sub1-AG1-AG2 | 5.2 | 11.6 | 13.8 | 6.5 | 12.8 | 14.8 | 0.4 | 0.6 | 1.2 | 0.5 | 1.0 | 2.2 | ||||
| Ciherang-Sub1 | 3.3 | 12.7 | 15.4 | 7.4 | 10.4 | 16.2 | 0.1 | 0.3 | 0.3 | 0.2 | 0.8 | 0.6 | ||||
| Ma-Zhan Red | 7.9 | 16.0 | 15.5 | 12.4 | 20.1 | 29.4 | 0.8 | 1.4 | 4.1 | 1.6 | 3.8 | 3.9 | ||||
| Mean | 5.4 | 13.5 | 14.9 | 7.6 | 13.1 | 19.8 | 0.5 | 0.8 | 1.6 | 0.8 | 1.6 | 2.2 | ||||
| HSD0.05 | 3.53** | 0.46*** | ||||||||||||||
| Dry season in 2017 | ||||||||||||||||
| Ciherang-Sub1-AG1 | 5.0 | 11.9 | 15.9 | 8.2 | 9.8 | 17.3 | 3.9 | 3.6 | 13.4 | 4.0 | 5.6 | 7.5 | ||||
| Ciherang-Sub1-AG2 | 8.5 | 12.8 | 23.3 | 6.2 | 15.5 | 19.7 | 3.4 | 5.1 | 9.6 | 5.9 | 8.8 | 6.6 | ||||
| Ciherang-Sub1-AG1-AG2 | 8.0 | 11.4 | 16.3 | 7.1 | 12.4 | 18.9 | 5.1 | 8.2 | 12.6 | 7.8 | 10.5 | 10.7 | ||||
| Ciherang-Sub1 | 6.1 | 10.3 | 17.0 | 6.7 | 10.5 | 17.9 | 1.9 | 2.1 | 4.5 | 1.7 | 2.5 | 2.9 | ||||
| Ma-Zhan Red | 8.6 | 15.0 | 22.5 | 10.0 | 18.4 | 24.2 | 4.2 | 11.7 | 14.8 | 6.4 | 11.1 | 13.5 | ||||
| Mean | 7.2 | 12.3 | 19.0 | 7.6 | 13.3 | 19.6 | 3.7 | 6.1 | 11.0 | 5.1 | 7.7 | 8.2 | ||||
| HSD0.05 | ns | 2.24* | ||||||||||||||
| Stem dry weight | Wet season in 2016 | |||||||||||||||
| Ciherang-Sub1-AG1 | 3.9 | 10.2 | 12.0 | 5.8 | 12.0 | 16.8 | 0.3 | 0.8 | 1.4 | 0.6 | 1.0 | 2.3 | ||||
| Ciherang-Sub1-AG2 | 4.1 | 11.1 | 11.6 | 5.6 | 11.2 | 20.9 | 0.6 | 1.3 | 2.1 | 0.9 | 1.5 | 2.7 | ||||
| Ciherang-Sub1-AG1-AG2 | 5.1 | 10.0 | 14.2 | 5.5 | 10.6 | 15.2 | 0.3 | 0.8 | 2.0 | 0.7 | 1.5 | 2.5 | ||||
| Ciherang-Sub1 | 3.3 | 10.9 | 11.3 | 5.5 | 9.6 | 14.3 | 0.1 | 0.3 | 0.6 | 0.2 | 0.7 | 0.6 | ||||
| Ma-Zhan Red | 8.3 | 16.1 | 18.2 | 11.1 | 16.0 | 21.8 | 0.7 | 1.6 | 4.4 | 2.1 | 2.7 | 3.5 | ||||
| Mean | 4.9 | 11.7 | 13.5 | 6.7 | 11.9 | 17.8 | 0.4 | 1.0 | 2.1 | 0.9 | 1.5 | 2.3 | ||||
| HSD0.05 | ns | 0.47* | ||||||||||||||
| Dry season in 2017 | ||||||||||||||||
| Ciherang-Sub1-AG1 | 4.5 | 11.5 | 15.3 | 7.7 | 8.9 | 15.6 | 3.5 | 4.7 | 13.0 | 3.9 | 5.4 | 7.1 | ||||
| Ciherang-Sub1-AG2 | 7.2 | 12.9 | 21.9 | 5.7 | 14.7 | 18.6 | 3.2 | 6.3 | 9.2 | 5.5 | 8.3 | 5.7 | ||||
| Ciherang-Sub1-AG1-AG2 | 7.2 | 10.7 | 14.2 | 6.3 | 12.1 | 17.4 | 4.8 | 8.8 | 13.1 | 7.8 | 10.2 | 10.7 | ||||
| Ciherang-Sub1 | 6.2 | 9.5 | 16.4 | 6.1 | 10.0 | 17.0 | 1.6 | 2.2 | 4.5 | 1.6 | 2.6 | 2.4 | ||||
| Ma-Zhan Red | 7.4 | 12.3 | 19.2 | 8.7 | 16.1 | 22.2 | 3.7 | 9.8 | 13.9 | 5.8 | 9.3 | 12.1 | ||||
| Mean | 6.5 | 11.4 | 17.4 | 6.9 | 12.4 | 18.2 | 3.4 | 6.4 | 10.8 | 4.9 | 7.2 | 7.6 | ||||
| HSD0.05 | ns | ns | ||||||||||||||
| Genotype | Saturated | |||||||
|---|---|---|---|---|---|---|---|---|
| D1 | D2 | |||||||
| Wet season-2016 | 4 g/m2 | 8 g/m2 | 12 g/m2 | 4 g/m2 | 8 g/m2 | 12 g/m2 | ||
| Ciherang-Sub1-AG1 | 1.91 | 4.97 | 7.10 | 2.66 | 5.18 | 8.94 | ||
| Ciherang-Sub1-AG2 | 2.03 | 4.53 | 6.62 | 2.87 | 6.24 | 9.91 | ||
| Ciherang-Sub1-AG1-AG2 | 2.92 | 5.56 | 7.43 | 2.65 | 5.25 | 7.34 | ||
| Ciherang-Sub1 | 2.33 | 4.42 | 5.96 | 2.52 | 4.29 | 6.95 | ||
| Ma-Zhan Red | 3.25 | 6.90 | 7.68 | 3.59 | 6.72 | 8.58 | ||
| Mean | 2.5 | 5.3 | 7.0 | 2.9 | 5.5 | 8.3 | ||
| LSD 0.05 | ns | ns | ns | ns | ns | ns | ||
| Flood | ||||||||
| Ciherang-Sub1-AG1 | 0.12 | 0.34 | 0.78 | 0.32 | 0.43 | 1.14 | ||
| Ciherang-Sub1-AG2 | 0.44 | 0.57 | 1.25 | 0.53 | 0.66 | 1.38 | ||
| Ciherang-Sub1-AG1-AG2 | 0.24 | 0.42 | 1.24 | 0.35 | 0.86 | 1.35 | ||
| Ciherang-Sub1 | 0.10 | 0.17 | 0.38 | 0.07 | 0.40 | 0.41 | ||
| Ma-Zhan Red | 0.34 | 0.91 | 2.05 | 0.62 | 1.00 | 1.99 | ||
| Mean | 0.2 | 0.5 | 1.1 | 0.4 | 0.7 | 1.3 | ||
| LSD 0.05 | ns | ns | ns | ns | ns | ns | ||
| Dry season 2017 | Saturated | |||||||
| Ciherang-Sub1-AG1 | 1.65 | 4.93 | 7.16 | 4.18 | 3.59 | 7.81 | ||
| Ciherang-Sub1-AG2 | 3.84 | 6.73 | 10.29 | 2.48 | 8.60 | 7.41 | ||
| Ciherang-Sub1-AG1-AG2 | 3.76 | 5.94 | 8.45 | 2.87 | 5.68 | 11.30 | ||
| Ciherang-Sub1 | 2.58 | 3.35 | 6.50 | 2.49 | 4.47 | 10.94 | ||
| Ma-Zhan Red | 2.95 | 5.70 | 7.32 | 3.14 | 5.85 | 8.35 | ||
| Mean | 3.0 | 5.3 | 7.9 | 3.0 | 5.6 | 9.2 | ||
| LSD 0.05 | ns | ns | ns | ns | ns | ns | ||
| Flood | ||||||||
| Ciherang-Sub1-AG1 | 2.34 | 1.85 | 6.63 | 2.64 | 2.88 | 4.03 | ||
| Ciherang-Sub1-AG2 | 1.70 | 2.73 | 5.11 | 3.53 | 4.05 | 3.28 | ||
| Ciherang-Sub1-AG1-AG2 | 1.93 | 4.27 | 7.46 | 4.89 | 6.30 | 6.56 | ||
| Ciherang-Sub1 | 1.02 | 1.20 | 2.69 | 0.87 | 1.70 | 1.94 | ||
| Ma-Zhan Red | 1.27 | 5.37 | 6.03 | 2.07 | 3.71 | 7.71 | ||
| Mean | 1.7 | 3.1 | 5.6 | 2.8 | 3.7 | 4.7 | ||
| LSD 0.05 | 1.14* | 1.14* | 1.14* | 1.14* | 1.14* | 1.14* | ||
Supplemental Table 4. Root dry weight (g/m2) of 21 DOS with different seeding rate and depth, under (saturated) control and flooded condition in field trials conducted during wet season 2016 and dry season 2017.
| Genotype | Saturated | |||||||
|---|---|---|---|---|---|---|---|---|
| D1 | D2 | |||||||
| Wet season-2016 | 4 g/m2 | 8 g/m2 | 12 g/m2 | 4 g/m2 | 8 g/m2 | 12 g/m2 | ||
| Ciherang-Sub1-AG1 | 1.91 | 4.97 | 7.10 | 2.66 | 5.18 | 8.94 | ||
| Ciherang-Sub1-AG2 | 2.03 | 4.53 | 6.62 | 2.87 | 6.24 | 9.91 | ||
| Ciherang-Sub1-AG1-AG2 | 2.92 | 5.56 | 7.43 | 2.65 | 5.25 | 7.34 | ||
| Ciherang-Sub1 | 2.33 | 4.42 | 5.96 | 2.52 | 4.29 | 6.95 | ||
| Ma-Zhan Red | 3.25 | 6.90 | 7.68 | 3.59 | 6.72 | 8.58 | ||
| Mean | 2.5 | 5.3 | 7.0 | 2.9 | 5.5 | 8.3 | ||
| LSD 0.05 | ns | ns | ns | ns | ns | ns | ||
| Flood | ||||||||
| Ciherang-Sub1-AG1 | 0.12 | 0.34 | 0.78 | 0.32 | 0.43 | 1.14 | ||
| Ciherang-Sub1-AG2 | 0.44 | 0.57 | 1.25 | 0.53 | 0.66 | 1.38 | ||
| Ciherang-Sub1-AG1-AG2 | 0.24 | 0.42 | 1.24 | 0.35 | 0.86 | 1.35 | ||
| Ciherang-Sub1 | 0.10 | 0.17 | 0.38 | 0.07 | 0.40 | 0.41 | ||
| Ma-Zhan Red | 0.34 | 0.91 | 2.05 | 0.62 | 1.00 | 1.99 | ||
| Mean | 0.2 | 0.5 | 1.1 | 0.4 | 0.7 | 1.3 | ||
| LSD 0.05 | ns | ns | ns | ns | ns | ns | ||
| Dry season 2017 | Saturated | |||||||
| Ciherang-Sub1-AG1 | 1.65 | 4.93 | 7.16 | 4.18 | 3.59 | 7.81 | ||
| Ciherang-Sub1-AG2 | 3.84 | 6.73 | 10.29 | 2.48 | 8.60 | 7.41 | ||
| Ciherang-Sub1-AG1-AG2 | 3.76 | 5.94 | 8.45 | 2.87 | 5.68 | 11.30 | ||
| Ciherang-Sub1 | 2.58 | 3.35 | 6.50 | 2.49 | 4.47 | 10.94 | ||
| Ma-Zhan Red | 2.95 | 5.70 | 7.32 | 3.14 | 5.85 | 8.35 | ||
| Mean | 3.0 | 5.3 | 7.9 | 3.0 | 5.6 | 9.2 | ||
| LSD 0.05 | ns | ns | ns | ns | ns | ns | ||
| Flood | ||||||||
| Ciherang-Sub1-AG1 | 2.34 | 1.85 | 6.63 | 2.64 | 2.88 | 4.03 | ||
| Ciherang-Sub1-AG2 | 1.70 | 2.73 | 5.11 | 3.53 | 4.05 | 3.28 | ||
| Ciherang-Sub1-AG1-AG2 | 1.93 | 4.27 | 7.46 | 4.89 | 6.30 | 6.56 | ||
| Ciherang-Sub1 | 1.02 | 1.20 | 2.69 | 0.87 | 1.70 | 1.94 | ||
| Ma-Zhan Red | 1.27 | 5.37 | 6.03 | 2.07 | 3.71 | 7.71 | ||
| Mean | 1.7 | 3.1 | 5.6 | 2.8 | 3.7 | 4.7 | ||
| LSD 0.05 | 1.14* | 1.14* | 1.14* | 1.14* | 1.14* | 1.14* | ||
| Genotype | Yield (t/hm2) | Straw yield (t/hm2) | ||
|---|---|---|---|---|
| Control | Flood | Control | Flood | |
| Wet season-2016 | ||||
| Ciherang-Sub1+AG1 | 2.8 | 1.8 | 7.48 | 5.82 |
| Ciherang-Sub1+AG2 | 3.48 | 2.49 | 5.86 | 6.49 |
| Ciherang-Sub1+AG1+AG2 | 3.77 | 3.4 | 5.38 | 7.2 |
| Ciherang-Sub1 | 3.1 | 1.36 | 5.8 | 4.72 |
| KHO | 3.01 | 2.42 | 8.34 | 9.59 |
| Ma-Zhan Red | 2.59 | 2.08 | 8.69 | 8.35 |
| Mean | 3.13 | 2.26 | 6.93 | 7.03 |
| HSD0.05 | 0.92* | 0.72** | 2.38* | 1.40** |
| Dry season-2017 | ||||
| PSB Rc82-AG1 | 6.26 | 4.51 | 7.16 | 6.89 |
| PSB Rc82-AG2 | 6.86 | 4.13 | 7.62 | 6.70 |
| PSB Rc82-AG1+AG2 | 6.67 | 4.59 | 7.13 | 5.69 |
| Ciherang-Sub1+AG1 | 5.95 | 3.81 | 10.99 | 5.37 |
| Ciherang-Sub1+AG2 | 5.19 | 4.29 | 9.51 | 7.17 |
| Ciherang-Sub1+AG1+AG2 | 6.04 | 4.65 | 10.58 | 7.66 |
| PSB Rc82 | 6.68 | 2.76 | 7.44 | 4.89 |
| Ciherang-Sub1 | 6.71 | 3.27 | 7.62 | 5.88 |
| KHO | 2.90 | 2.53 | 9.42 | 8.43 |
| Ma-Zhan Red | 3.72 | 3.50 | 8.68 | 8.65 |
| Mean | 5.70 | 3.92 | 8.61 | 6.71 |
| HSD0.05 | 0.78** | 0.94** | 2.23** | 1.92** |
Supplemental Table 5. Grain yield and straw yield of AG-lines under control and flooded conditions during wet season 2016 and dry season 2017.
| Genotype | Yield (t/hm2) | Straw yield (t/hm2) | ||
|---|---|---|---|---|
| Control | Flood | Control | Flood | |
| Wet season-2016 | ||||
| Ciherang-Sub1+AG1 | 2.8 | 1.8 | 7.48 | 5.82 |
| Ciherang-Sub1+AG2 | 3.48 | 2.49 | 5.86 | 6.49 |
| Ciherang-Sub1+AG1+AG2 | 3.77 | 3.4 | 5.38 | 7.2 |
| Ciherang-Sub1 | 3.1 | 1.36 | 5.8 | 4.72 |
| KHO | 3.01 | 2.42 | 8.34 | 9.59 |
| Ma-Zhan Red | 2.59 | 2.08 | 8.69 | 8.35 |
| Mean | 3.13 | 2.26 | 6.93 | 7.03 |
| HSD0.05 | 0.92* | 0.72** | 2.38* | 1.40** |
| Dry season-2017 | ||||
| PSB Rc82-AG1 | 6.26 | 4.51 | 7.16 | 6.89 |
| PSB Rc82-AG2 | 6.86 | 4.13 | 7.62 | 6.70 |
| PSB Rc82-AG1+AG2 | 6.67 | 4.59 | 7.13 | 5.69 |
| Ciherang-Sub1+AG1 | 5.95 | 3.81 | 10.99 | 5.37 |
| Ciherang-Sub1+AG2 | 5.19 | 4.29 | 9.51 | 7.17 |
| Ciherang-Sub1+AG1+AG2 | 6.04 | 4.65 | 10.58 | 7.66 |
| PSB Rc82 | 6.68 | 2.76 | 7.44 | 4.89 |
| Ciherang-Sub1 | 6.71 | 3.27 | 7.62 | 5.88 |
| KHO | 2.90 | 2.53 | 9.42 | 8.43 |
| Ma-Zhan Red | 3.72 | 3.50 | 8.68 | 8.65 |
| Mean | 5.70 | 3.92 | 8.61 | 6.71 |
| HSD0.05 | 0.78** | 0.94** | 2.23** | 1.92** |
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