Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice

Abstract

Abstract: The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T polymorphism. Among these alleles, (CT)₁₂-G, (CT)₁₅-G, (CT)₁₆-G, (CT)₁₇-G, (CT)₁₈-G and (CT)₂₁-G have not been reported. Seventy-two single-segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by using Huajingxian 74 with the (CT)₁₁-G allele as a recipient and 20 accessions containing 12 different alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 to 77.3 cM with an average of 17.4 cM.

Key words: rice, waxy gene, single segment substitution line, allelic variation, molecular marker-assisted selection

ZENG Rui-zhen, ZHANG Ze-min, HE Feng-hua, XI Zhang-ying, Akshay TALUKDAR, SHI Jun-qiong, QIN Li-jun, HUANG Chao-feng, ZHANG Gui-quan. Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice[J]. RICE SCIENCE, 2006, 13(1): 9-14 .

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