Effects of Formulated Fertilizer Synergist on Abscisic Acid Accumulation, 
Proline Content and Photosynthetic Characteristics of Rice under Drought

Abstract

Abstract: To investigate the effects of formulated fertilizer synergist on the drought tolerance in rice, pot experiment was
conducted to analyze the photosynthetic characteristics and the accumulation of abscisic acid (ABA) and proline in
middle-season rice variety Peiliangyou 93. The synergist could improve the net photosynthetic rate, and coordination
between the water loss and the CO₂ absorption as well as reduce the harmful effect on photosynthetic process under drought
conditions. Under drought, the ABA accumulated massively both in roots and leaves, while the ABA content in roots was far
higher than that in leaves. The results indicate that synergist could increase the ABA accumulation, but reduce the proline
accumulation in rice plant under drought.

Key words: fertilizer synergist, drought, abscisic acid, proline, photosynthetic characteristics

WANG Shao-xian, XIA Shi-tou, PENG Ke-qin, KUANG Feng-chun, CAO Yong, XIAO Lang-tao. Effects of Formulated Fertilizer Synergist on Abscisic Acid Accumulation,
Proline Content and Photosynthetic Characteristics of Rice under Drought[J]. RICE SCIENCE, 2007, 14(1): 42-48 .

References

1 Parry M A J, John A P, Khan S, Lea P J, Keys A J. Rubisco activity: Effects of drought stress. Ann Bot, 2002, 89:833-839.
2 Griffiths H, Parry M A J. Plant responses to water stress. Ann Bot, 2002, 89: 801-802.
3 Wang Z L, Mambelli S, Setter T L. Abscisic acid catabolism in maize kernels in response to water deficit at early endosperm development. Ann Bot, 2002, 90: 623-630.
4 Yu L X, Setter T L. Comparative transcriptional profiling of placenta and endosperm in developing maize kernels in response to water deficit. Plant Physiol, 2003, 131: 568-582.
5 Fan X R, Sheng Q R, Cui G X, Xu G H. Effect of soil water regime on dynamic levels of endogenous hormones and relationship between hormones and physio-biochemistry and morphology of rice of different cultivars cultivated in upland soil. Acta Ped Sin, 2002, 39(2): 206-213. (in Chinese with English abstract)
6 Chaves M M, Pereira J S, Maroco J, Rodrigues M L, Ricardo C P P, Osorio M L. Carvalho I, Faria T, Pinheiro C. How plants cope with water stress in the field? Photosynthesis and growth. Ann Bot, 2002, 89: 907-916.
7 Jia W S, Wang Y Q, Zhang S Q, Zhang J H. Salt-stress-induced ABA accumulation is more sensitively triggered in roots than in shoots. J Exp Bot, 2002, 53: 2201-2206.
8 Borel C, Audran C, Frey A, Marion-Poll A, Tardieu F, Simonneau T. N. Plumbaginifolia zeaxanthin epoxidase transgenic lines have unaltered baseline ABA accumulations in roots and xylem sap, but contrasting sensitivities of ABA accumulation to water deficit. J Exp Bot, 2001, 52: 427-434.
9 Sharp R E, Lenoble M E. ABA, ethylene and the control of shoot and root growth under water stress. J Exp Bot, 2002, 53: 33-37.
10 Wang S X, Peng K Q. Advances of study in mechanism of ABA biosynthesis and its signal transduction under stresses. J Hunan Agric Univ: Nat Sci, 2002, 28: 55-61. (in Chinese with English abstract)
11 Hare P D, Cress W A, van Staden J. Proline synthesis and degradation: A model system for elucidating stress-related signal transduction. J Exp Bot, 1999, 50(333): 413-434.
12 Yamada M, Morishita H, Urano K, Shiozaki N, Yamaguchi- Shinozaki K, Shinozaki K, Yoshiba Y. Effects of free proline accumulation in petunias under drought stress. J Exp Bot, 2005, 56(417): 1975-1981.
13 Thiery L, Leprince A S, Lefebvre D, Ghars M A, Debarbieux E, Savoure A. Phospholipase D is a negative regulator of proline biosynthesis in Arabidopsis thaliana. J Biol Chem, 2004, 15(279): 14812-14818.
14 Wang S X, Peng K Q, Xiao L T, Tong J H, Wang R Z. Study on application of synergist to rice special fertilizers and its mechanism. Plant Nutr Fert Sci, 2003, 9(3): 294-298.
15 Li H S. Principle and Technology for Plant Physiological and Biochemical Experiment. Beijing: Higher Education Press, 2001.
16 Wang R Z, Xiao L T, Lin W H, Cao Y. High performance liquid chromatographic determination of internal hormones in inter subspecific hybrid rice. Chinese J Chromatography, 2002, 20(2): 148-150. (in Chinese with English abstract)
17 Zou Q. The photosynthesis of crop in water stresses. Crops, 1994(5): 263-267. (in Chinese)
18 Sauter A, Davies W J, Hartung W. The long-distance abscisic acid signal in the droughted plant: The fate of the hormone on its way from root to shoot. J Exp Bot, 2001, 52(363): 1991-1997.
19 Morillon R, Chrispeels M J. The role of ABA and the transpiration stream in the regulation of the osmotic water permeability of leaf cells. PNAS, 2001, 98(24): 14138-14143.
20 Xia Y. Effects of water stress on changes of proline and chlorophyll on fruit crops. J Gansu Agric Univ, 1993(1): 26-31. (in Chinese with English abstract)
21 Wang S J, Guo B. The relationship between the accumulations of the abscisc acid and free proline in wheatgrass under water stress. J Gansu Agric Univ, 1993(1): 36-40. (in Chinese with English abstract)

[1]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[2]	Jiang Changjie, Liang Zhengwei, Xie Xianzhi. Priming for Saline-Alkaline Tolerance in Rice: Current Knowledge and Future Challenges [J]. Rice Science, 2023, 30(5): 417-425.
[3]	Yang Xiaozheng, Liu Yusong, Huang Jing, Tao Ye, Wang Yifeng, Shen Renfang, Zhu Xiaofang. NaCl Facilitates Cell Wall Phosphorus Reutilization in Abscisic Acid Dependent Manner in Phosphorus Deficient Rice Root [J]. Rice Science, 2023, 30(2): 138-147.
[4]	Liu Yantong, Li Ting, Jiang Zhishu, Zeng Chuihai, He Rong, Qiu Jiao, Lin Xiaoli, Peng Limei, Song Yongping, Zhou Dahu, Cai Yicong, Zhu Changlan, Fu Junru, He Haohua, Xu Jie. Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding [J]. Rice Science, 2022, 29(6): 522-534.
[5]	Kossi Lorimpo Adjah, Maxwell Darko Asante, Aboubacar Toure, Mawuli Aziadekey, Francis Osei Amoako-Andoh, Michael Frei, Yacouba Diallo, Komi Agboka. Improvement of Rice Production under Drought Conditions in West Africa: Application of QTLs in Breeding for Drought Resistance [J]. Rice Science, 2022, 29(6): 512-521.
[6]	Gao Xiuying, Zhang Ye, Zhang Hongsheng, Huang Ji. A β-ketoacyl-CoA Synthase OsCUT1 Confers Increased Drought Tolerance in Rice [J]. Rice Science, 2022, 29(4): 353-362.
[7]	Liu Kai, Li Minjuan, Zhang Bin, Yin Xuming, Xia Xinjie, Wang Manling, Cui Yanchun. Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice [J]. Rice Science, 2022, 29(3): 257-267.
[8]	Chen Eryong, Shen Bo. OsABT, a Rice WD40 Domain-Containing Protein, Is Involved in Abiotic Stress Tolerance [J]. Rice Science, 2022, 29(3): 247-256.
[9]	Muhammad Mahran Aslam, Muhammad Abdul Rehman Rashid, Mohammad Aquil Siddiqui, Muhammad Tahir Khan, Fozia Farhat, Shafquat Yasmeen, Imtiaz Ahmad Khan, Shameem Raja, Fatima Rasool, Mahboob Ali Sial, Zhao Yan. Recent Insights into Signaling Responses to Cope Drought Stress in Rice [J]. Rice Science, 2022, 29(2): 105-117.
[10]	Balija Vishalakshi, Bangale Umakanth, Ponnuvel Senguttuvel, Makarand Barbadikar Kalyani, Prasad Madamshetty Srinivas, Rao Durbha Sanjeeva, Yadla Hari, Madhav Maganti Sheshu. Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production [J]. Rice Science, 2021, 28(5): 493-500.
[11]	Chuxin Wang, Chengchao Zhu, Yu Zhou, Min Xiong, Jindong Wang, Huang Bai, Chenya Lu, Changquan Zhang, Qiaoquan Liu, Qianfeng Li. OsbZIP09, a Unique OsbZIP Transcription Factor of Rice, Promotes Rather Than Suppresses Seed Germination by Attenuating Abscisic Acid Pathway [J]. Rice Science, 2021, 28(4): 358-367.
[12]	Silas Akos Ibrahim, Y. Rafii Mohd, Razi Ismail Mohd, Izan Ramlee Shairul, Abd Aziz Shamsudin Noraziyah, Ramli Asfaliza, Chibuike Chukwu Samuel, Swaray Senesie, Jalloh Momodu. Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines [J]. Rice Science, 2021, 28(3): 279-288.
[13]	Vera Jesus Da Costa Maria, Ramegowda Yamunarani, Ramegowda Venkategowda, N. Karaba Nataraja, M. Sreeman Sheshshayee, Udayakumar Makarla. Combined Drought and Heat Stress in Rice: Responses, Phenotyping and Strategies to Improve Tolerance [J]. Rice Science, 2021, 28(3): 233-242.
[14]	Baoxiang Wang, Yan Liu, Yifeng Wang, Jingfang Li, Zhiguang Sun, Ming Chi, Yungao Xing, Bo Xu, Bo Yang, Jian Li, Jinbo Liu, Tingmu Chen, Zhaowei Fang, Baiguan Lu, Dayong Xu, Kazeem Bello Babatunde. OsbZIP72 Is Involved in Transcriptional Gene-Regulation Pathway of Abscisic Acid Signal Transduction by Activating Rice High-Affinity Potassium Transporter OsHKT1;1 [J]. Rice Science, 2021, 28(3): 257-267.
[15]	Panda Debabrata, Sakambari Mishra Swati, Kumar Behera Prafulla. Drought Tolerance in Rice: Focus on Recent Mechanisms and Approaches [J]. Rice Science, 2021, 28(2): 119-132.