Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2

doi:10.1016/j.rsci.2017.08.004

Abstract

Abstract:

Bacterial leaf blight of rice (BLB), caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent. IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC₃F₃ lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines (BC₃F₃ 5.1.5.1, BC₃F₃ 5.1.5.12, BC₃F₃ 8.5.6.44, BC₃F₃ 9.5.4.1 and BC₃F₃ 9.5.4.23) were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security.

Key words: rice, backcrossing, bacterial leaf blight, marker-assisted selection

Thi Nguyen Hue, Hong Vu Quang, Van Mai Tan, Thi Nguyen Thu, Duc Vu Lam, Thanh Nguyen Tung, Viet Nguyen Long, Thu Thi Vu Hien, Thi Nong Hue, Nguyen Dinh Trung, Toshitsugu Nakano, Van Vu Liet. Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2[J]. Rice Science, 2018, 25(1): 52-56.

Figures/Tables 5

References 23

[1]	Adhikari T B, Mew T W, Leach J E.1999. Genotypic and pathotypic diversity inXanthomonas oryzae pv. oryzae in Nepal. Phythopathol, 89(8): 687-694.
[2]	Cao L Y, Zhan X D, Zhuang J Y, Cheng S H.2005. Breeding of indica hybrid rice Guodao 1 with good quality, high yield and resistance to bacterial leaf blight by marker-assisted selection technique. Hybrid Rice, 20(3): 16-18. (in Chinese with English abstract)
[3]	Chen S, Zhang Q F.2000. Improvement of bacterial blight resistance of hybrid rice by molecular marker-assisted selection.J Huazhong Agric Univ, 19(3): 183-189. (in Chinese with English abstract)
[4]	Chen S, Lin X H, Xu C G, Zhang Q F.2000. Improvement of bacterial blight resistance of ‘Minghui 63’ an elite restorer line of hybrid rice, by molecular marker-assisted selection.Crop Sci, 40: 239-244.
[5]	Furuya N, Taura S, Goto T, Thuy B T, Ton P H, Tsuchiya K, Yoshimura A.2012. Diversity in virulence ofXanthomonas oryzae pv. oryzae from Northern Vietnam. Jpn Agric Res Quart, 46(4): 329-338.
[6]	Huang N, Angeles E R, Domingo J, Magpatay G, Singh S, Zhang G, Kumaravadevil N, Bennett J, Khush G S.1997. Pyramiding of bacterial blight resistance genes in rice: Marker assisted selection using RFLP and PCR.Theor Appl Genet, 95(3): 313-320.
[7]	Juliano B O.1971. A simplified assay for milled-rice amylose.Cereal Sci Today, 16: 334-340.
[8]	Khan M A, Naeem M, Iqbal M.2014. Breeding approaches for bacterial leaf blight resistance in rice (Oryza sativa L.), current status and future directions. Eur J Plant Pathol, 139(1): 27-37.
[9]	Khush G S, Mackill D J, Sidhu G S.1989. Breeding rice for resistance to bacterial blight. In: IRRI. Bacterial Blight of Rice. Manila, the Philippines: International Rice Research Institute: 207-217.
[10]	Kibria K, Iskam M M, Begum S N.2008. Screening of aromatics rice lines by phenotypic and molecular markers.Bangl J Bot, 37: 141-147.
[11]	Lowry O H, Rosebrough N J, Farr A L, Randall R J.1951. Protein measurement with the folin phenol reagent.Biol Chem, 193(1): 265-275.
[12]	Luo Y C, Wang S H, Li C Q, Wu S, Wang D Z, Du S Y.2005. Improvement of bacterial blight resistance by molecular marker-assisted selection in a wide compatibility restorer line of hybrid rice.Chin J Rice Sci, 19(1): 36-40. (in Chinese with English abstract)
[13]	Mishra D, Vishnupriya M R, Anil M G, Konda K, Raj Y, Sonti R V.2013. Pathotype and genetic diversity amongst Indian isolates of Xanthomonas oryzae pv. oryzae. PLoS One, 8(11): e81996.
[14]	Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Miobe Y.2002. Positional cloning of rice semidwarf gene, sd-1: Rice “green revolution gene” encodes a mutant enzyme involved in gibberellin synthesis. DNA Res, 9(1): 11-17.
[15]	NiNo-Liu D O, Ronald P C, Bogdanove A J.2006. Xanthomonas oryzae pathovars: Model pathogens of a model crop. Mol Plant Pathol, 7(5): 303-324.
[16]	Perez L M, Redona E D, Mendioro M S, Cruz C M V, Leung H.2008. Introgression ofXa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice(Oryza sativa L.) for the development of two-line hybrids. Euphytica, 164: 627-636.
[17]	Ronald P C, Albano B, Tabien R, Abenes L, Wu K S, McCouch S, Tankley S D.1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Mol Gen Genet, 236(1): 113-120.
[18]	Singh S, Sidhu J S, Huang N, Vikal Y, Li Z, Brar D S, Dhaliwal H S, Khush G S.2001. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet, 102: 1011-1015.
[19]	Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Gardner J, Wang B, Zhai W X, Zhu L H, Fauquet C, Ronald P C.1995. A receptor kinase-like protein encoded by the rice disease resistance geneXa27. Science, 270: 1804-1806.
[20]	Song W Y, Pi L Y, Wang G L, Gardner J, Holsten T, Ronald P C.1997. Evolution of the rice Xa21 disease resistance gene family. Plant Cell, 9(8): 1279-1287.
[21]	Tong H J, Xue Q Z.2003. II You 8220, a new disease resistant hybrid rice combination.Hybrid Rice, 18(5): 73-74. (in Chinese with English abstract)
[22]	Williams C E, Wang B, Holsten T E, Scambray J, de Assis Goes da Silva F, Ronald P C.1996. Markers for selection of the rice Xa21 disease resistance gene. Theor Appl Genet, 93: 1119-1122.
[23]	Zheng K L, Shen B, Qian H R.1991. DNA polymorphism generated by arbitrary primed PCR in rice.Rice Genet Newsl, 8: 134-136.

Accession	981.HUA10146		996.HUA10147		1035.HUA10153
Accession	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction
LT2 (CK)	17.5	S	21.1	S	29.6	S
IRBB21 (CK)	2.8	R	3.9	R	16.5	S
IR24 (CK)	21.2	S	24.1	S	28.4	S
BC₃F₂ 5.1.5.1	4.5	R	7.1	R	11.6	M
BC₃F₂ 5.1.5.11	5.3	R	5.5	R	10.6	M
BC₃F₂ 5.1.5.12	6.8	R	7.6	R	9.8	M
BC₃F₂ 5.1.5.18	6.9	R	7.6	R	27.5	S
BC₃F₂ 8.5.6.21	6.5	R	6.8	R	19.5	S
BC₃F₂ 8.5.6.37	4.7	R	6.5	R	21.1	S
BC₃F₂ 8.5.6.44	6.2	R	6.7	R	28.3	S
BC₃F₂ 9.5.4.1	6.4	R	6.3	R	19.4	S
BC₃F₂ 9.5.4.23	4.6	R	4.3	R	21.1	S
BC₃F₂ 9.5.4.29	7.6	R	8	R	28.1	S
BC₃F₂ 9.5.4.43	4.4	R	4.8	R	14.2	S

Accession	981.HUA10146		996.HUA10147		1035.HUA10153
Accession	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction
LT2 (CK)	17.5	S	21.1	S	29.6	S
IRBB21 (CK)	2.8	R	3.9	R	16.5	S
IR24 (CK)	21.2	S	24.1	S	28.4	S
BC₃F₂ 5.1.5.1	4.5	R	7.1	R	11.6	M
BC₃F₂ 5.1.5.11	5.3	R	5.5	R	10.6	M
BC₃F₂ 5.1.5.12	6.8	R	7.6	R	9.8	M
BC₃F₂ 5.1.5.18	6.9	R	7.6	R	27.5	S
BC₃F₂ 8.5.6.21	6.5	R	6.8	R	19.5	S
BC₃F₂ 8.5.6.37	4.7	R	6.5	R	21.1	S
BC₃F₂ 8.5.6.44	6.2	R	6.7	R	28.3	S
BC₃F₂ 9.5.4.1	6.4	R	6.3	R	19.4	S
BC₃F₂ 9.5.4.23	4.6	R	4.3	R	21.1	S
BC₃F₂ 9.5.4.29	7.6	R	8	R	28.1	S
BC₃F₂ 9.5.4.43	4.4	R	4.8	R	14.2	S

Accession	981.HUA10146		996.HUA10147		1035.HUA10153
Accession	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction
LT2 (CK)	17.5	S	21.1	S	29.6	S
IRBB21 (CK)	2.8	R	3.9	R	11.2	M
IR24 (CK)	21.2	S	24.1	S	33.2	S
BC₃F₃ 5.1.5.1	5.3	R	6.8	R	14.6	S
BC₃F₃ 5.1.5.11	6.8	R	7.3	R	21	S
BC₃F₃ 5.1.5.12	3.1	R	4	R	10.8	M
BC₃F₃ 5.1.5.18	5.8	R	7.2	R	19.6	S
BC₃F₃ 8.5.6.21	7.1	R	8	R	24.1	S
BC₃F₃ 8.5.6.37	3.4	R	4.5	R	11.5	M
BC₃F₃ 8.5.6.44	5.4	R	6.2	R	12.6	S
BC₃F₃ 9.5.4.1	4.5	R	7.1	R	14.6	S
BC₃F₃ 9.5.4.23	4.2	R	5.7	R	15.1	S
BC₃F₃ 9.5.4.29	3.5	R	7.1	R	11.6	M
BC₃F₃ 9.5.4.43	4	R	4.7	R	15.1	S

Accession	981.HUA10146		996.HUA10147		1035.HUA10153
Accession	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction	Lesion length (cm)	Disease reaction
LT2 (CK)	17.5	S	21.1	S	29.6	S
IRBB21 (CK)	2.8	R	3.9	R	11.2	M
IR24 (CK)	21.2	S	24.1	S	33.2	S
BC₃F₃ 5.1.5.1	5.3	R	6.8	R	14.6	S
BC₃F₃ 5.1.5.11	6.8	R	7.3	R	21	S
BC₃F₃ 5.1.5.12	3.1	R	4	R	10.8	M
BC₃F₃ 5.1.5.18	5.8	R	7.2	R	19.6	S
BC₃F₃ 8.5.6.21	7.1	R	8	R	24.1	S
BC₃F₃ 8.5.6.37	3.4	R	4.5	R	11.5	M
BC₃F₃ 8.5.6.44	5.4	R	6.2	R	12.6	S
BC₃F₃ 9.5.4.1	4.5	R	7.1	R	14.6	S
BC₃F₃ 9.5.4.23	4.2	R	5.7	R	15.1	S
BC₃F₃ 9.5.4.29	3.5	R	7.1	R	11.6	M
BC₃F₃ 9.5.4.43	4	R	4.7	R	15.1	S

Accession	Growth duration (d)	Plant height (cm)	No. of tillers per plant	No. of grains per plant	1000-grain	Polished rice rate (%)	Gelatinization temperature (ºC)	Amylose content (%)	Protein content (%)	Aroma (Score)
Accession	Growth duration (d)	Plant height (cm)	No. of tillers per plant	No. of grains per plant	weight (g)	Polished rice rate (%)	Gelatinization temperature (ºC)	Amylose content (%)	Protein content (%)	Aroma (Score)
LT2 (CK)	134	109.4 a	7	885.0 a	20.6	65.3	65	17.2	8.85	3
BC₃F₃ 5.1.5.1	134	111.2 a	6.8	845.4 a	21.2	67.4	65	17.6	8.82	3.1
BC₃F₃ 5.1.5.12	135	113.6 ab	7.5	965.0 b	21.2	65.6	65	17.1	8.91	3.3
BC₃F₃ 8.5.6.44	134	112.1 a	6.5	872.0 a	21.1	66.2	65	17.1	8.58	3.1
BC₃F₃ 9.5.4.1	133	109.6 a	7.1	926.4 ab	20.8	67.1	65	17.3	8.7	3.3
BC₃F₃ 9.5.4.23	135	110.3 a	6.4	871.0 a	21	64.9	65	16.8	8.92	3