Diversity of dsRNA Viruses Infecting Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA

doi:10.1016/j.rsci.2017.09.002

Abstract

Abstract:

Rice sheath blight, caused by Rhizoctonia solani (Kühn), is a notorious soil-borne disease prevalent in many rice-growing regions. Although several sporadic studies of mycoviruses in R. solani AG-1 IA have been reported for single strain of R. solani AG-1 IA, there have been no reports describing the distribution and diversity of mycoviruses in natural populations. In this study, 43 R. solani AG-1 IA strains collected from different locations in China were examined for the presence of dsRNA elements to confirm the presence of viral infections. Electrophoretypes showed that 16 of the 43 fungal strains (37.2%) contained dsRNAs that can be characterized as viruses. Furthermore, the species-specific reverse transcription PCR (RT-PCR) showed dsRNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections. Thus, our findings indicate mycoviruses infecting R. solani AG-1 IA in China are diverse, widespread and universal.

Key words: mycovirus, Rhizoctonia solani, reverse transcription polymerase chain reaction, diversity, dsRNA, rice sheath blight

Li Zheng, Chen Liu, Meiling Zhang, Mei Yang, Erxun Zhou. Diversity of dsRNA Viruses Infecting Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA[J]. Rice Science, 2018, 25(1): 57-60.

Figures/Tables 4

References 22

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Isolate No.	Location ^a	Isolate No.	Location^a
A5	Danzhou, Hainan	B315	Wuhua, Guangdong
A90	Danzhou, Hainan	B316	Wuhua, Guangdong
A92	Danzhou, Hainan	B331	Lianzhou, Guangdong
A107	Ledong, Hainan	C40	Minhou, Fujian
A132	Haikou, Hainan	D164	Hechi, Guangxi
A142	Haikou, Hainan	D168	Yangshuo, Guangxi
A154	Wenchang, Hainan	D175	Yangshuo, Guangxi
A182	Tunchang, Hainan	D181	Baise, Guangxi
B120	Dongyuan, Guangdong	D186	Baise, Guangxi
B237	Maoming, Guangdong	E51	Dali, Yunnan
B240	Maoming, Guangdong	GD-2	Lechang, Guangdong
B246	Suixi, Guangdong	GD-11	Ruyuan, Guangdong
B249	Suixi, Guangdong	GD-61	Guangzhou, Guangdong
B250	Suixi, Guangdong	GD-118	Doumen, Guangdong
B255	Suixi, Guangdong	H1	Xuancheng, Anhui
B261	Conghua, Guangdong	H2	Chaohu, Anhui
B265	Conghua, Guangdong	H3	Jingzhou, Hunan
B266	Conghua, Guangdong	N1	Hangzhou, Zhejiang
B270	Xinxing, Guangdong	X-5	Yangzhou, Jiangsu
B275	Taishan, Guangdong	Y1	Hangzhou, Zhejiang
B278	Taishan, Guangdong	ZJG-15	Zhangjiagang, Jiangsu
B297	Lufeng, Guangdong

Isolate No.	Location ^a	Isolate No.	Location^a
A5	Danzhou, Hainan	B315	Wuhua, Guangdong
A90	Danzhou, Hainan	B316	Wuhua, Guangdong
A92	Danzhou, Hainan	B331	Lianzhou, Guangdong
A107	Ledong, Hainan	C40	Minhou, Fujian
A132	Haikou, Hainan	D164	Hechi, Guangxi
A142	Haikou, Hainan	D168	Yangshuo, Guangxi
A154	Wenchang, Hainan	D175	Yangshuo, Guangxi
A182	Tunchang, Hainan	D181	Baise, Guangxi
B120	Dongyuan, Guangdong	D186	Baise, Guangxi
B237	Maoming, Guangdong	E51	Dali, Yunnan
B240	Maoming, Guangdong	GD-2	Lechang, Guangdong
B246	Suixi, Guangdong	GD-11	Ruyuan, Guangdong
B249	Suixi, Guangdong	GD-61	Guangzhou, Guangdong
B250	Suixi, Guangdong	GD-118	Doumen, Guangdong
B255	Suixi, Guangdong	H1	Xuancheng, Anhui
B261	Conghua, Guangdong	H2	Chaohu, Anhui
B265	Conghua, Guangdong	H3	Jingzhou, Hunan
B266	Conghua, Guangdong	N1	Hangzhou, Zhejiang
B270	Xinxing, Guangdong	X-5	Yangzhou, Jiangsu
B275	Taishan, Guangdong	Y1	Hangzhou, Zhejiang
B278	Taishan, Guangdong	ZJG-15	Zhangjiagang, Jiangsu
B297	Lufeng, Guangdong

Strain	Location ^a	dsRNA segment observed
Strain	Location ^a	Number	Size (kb) ^b
B275	Taishan, Guangdong	2	1.7, 2.0
A5	Danzhou, Hainan	3	1.7, 1.8, 9.0
A154	Wenchang, Hainan	2		1.8, 1.9
A182	Tunchang, Hainan	2		1.8, 1.9
B240	Maoming, Guangdong	2		1.8, 2.0
B261	Conghua, Guangdong	1		1.8
B266	Conghua, Guangdong	4		1.8, 2.0, 2.3, 9.0
B297	Lufeng, Guangdong	2		1.8, 1.9
B331	Lianzhou, Guangdong	3		1.8, 2.0, 2.3
D168	Yangshuo, Guangxi	2		1.8, 2.0
D175	Yangshuo, Guangxi	2		1.8, 1.9
GD-11	Ruyuan, Guangdong	3		1.8, 2.0, 2.3
H1	Xuancheng, Anhui	2		1.7, 1.8
H2	Chaohu, Anhui	2		1.8, 1.9
N1	Hangzhou, Zhejiang	4		1.8, 2.0, 2.3, 6.5
Y1	Hangzhou, Zhejiang	3		1.8, 2.0, 6.4

Strain	Location ^a	dsRNA segment observed
Strain	Location ^a	Number	Size (kb) ^b
B275	Taishan, Guangdong	2	1.7, 2.0
A5	Danzhou, Hainan	3	1.7, 1.8, 9.0
A154	Wenchang, Hainan	2		1.8, 1.9
A182	Tunchang, Hainan	2		1.8, 1.9
B240	Maoming, Guangdong	2		1.8, 2.0
B261	Conghua, Guangdong	1		1.8
B266	Conghua, Guangdong	4		1.8, 2.0, 2.3, 9.0
B297	Lufeng, Guangdong	2		1.8, 1.9
B331	Lianzhou, Guangdong	3		1.8, 2.0, 2.3
D168	Yangshuo, Guangxi	2		1.8, 2.0
D175	Yangshuo, Guangxi	2		1.8, 1.9
GD-11	Ruyuan, Guangdong	3		1.8, 2.0, 2.3
H1	Xuancheng, Anhui	2		1.7, 1.8
H2	Chaohu, Anhui	2		1.8, 1.9
N1	Hangzhou, Zhejiang	4		1.8, 2.0, 2.3, 6.5
Y1	Hangzhou, Zhejiang	3		1.8, 2.0, 6.4