Agro-Morphological, Physico-Chemical and Molecular Characterization of Rice Germplasm with Similar Names of Bangladesh

doi:10.1016/j.rsci.2016.06.004

Abstract

Abstract:

Thirty-one duplicate and similar named rice germplasms of Bangladesh were studied to assess the genetic variation for the agro-morphological and physico-chemical traits and simple sequence repeat banding patterns during 2009-2012 at Bangladesh Rice Research Institute. The range of variations within the cultivar groups showed higher degree. The principal component analysis showed that the first five components with vector values > 1 contributed 82.90% of the total variations. The cluster analysis grouped the genotypes into four clusters, where no duplicate germplasm was found. The highest number (11) of genotypes was constellated in cluster I and the lowest (3) in cluster II. The intra- and inter-cluster distances were the maximum in cluster I (0.93) and between clusters I and IV (24.61), respectively, and the minimum in cluster IV (0.62) and between clusters I and III (5.07), respectively. The cluster mean revealed that the crosses between the genotypes of cluster I with those of clusters II and IV would exhibit high heterosis for maximum good characters. A total of 350 alleles varied from 3 (RM277) to 14 (RM21) with an average of 7.8 per locus were detected at 45 microsatellite loci across the 31 rice accessions. The gene diversity ranged from 0.48 to 0.90 with an average of 0.77, and the polymorphism information content values from 0.44 (RM133) to 0.89 (RM206) with an average of 0.74. RM206, RM21, RM55, RM258 and RM433 were considered as the best markers on the basis of their higher polymorphism information content values. The dendrogram from unweighted pair-group method with arithmetic average clustering also classified the genotypes into four groups, where group IV comprised of 20 genotypes and group III of one genotype, but no duplicate was found. Finally, similar and duplicate named rice germplasms need to be conserved in gene bank as are distinct from each other.

Key words: diversity, simple sequence repeat, similar name, landrace rice, agro-morphological and physico-chemical trait

Sharf Uddin Ahmed Mir, Khalequzzaman Mohammad, Khairul Bashar Md., Kalam Mohammad Shamsuddin Abul. Agro-Morphological, Physico-Chemical and Molecular Characterization of Rice Germplasm with Similar Names of Bangladesh[J]. Rice Science, 2016, 23(4): 211-218.

Figures/Tables 8

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Serial No.	Accession name	Accession No. ^a	Origin
KS1	Kartik Sail	3243	Sylhet
KS2	Kartik Sail	776	Chittagong
KS3	Katih Shail	438	Rajshahi
KS4	Kartik Sail	539	Rangpur
KS5	Kartik Sail	77	Dhaka
KS6	Kati Shail	170	Tangail
KS7	Kartik Sail	3662	Sherpur
KS8	Kati Shail	3631	Rajshahi
KS9	Kartika	4053	Sylhet
KS10	Kartik Sail	4881	Tangail
KS11	Kartik Sail	76	Dhaka
KS12	Kati Shail	437	Rajshahi
KS13	Kartik Sail	78	Dhaka
KS14	Kartik Sail	1882	Kishorganj
KS15	Kartik sail (2)	689	Comilla
KS16	Kartik sail (2)	846	Sylhet
KS17	Kartik Sail	664	Comilla
KS18	Kartik Sail	1887	Kishorganj
KS19	Kartik Sail	844	Sylhet
KS20	Katih Shail	994	Khulna
KS21	Kartik Sail	845	Sylhet
DB1	Dhali Boro	2250	Sylhet
DB2	Dhali Boro	2247	Sylhet
DB3	Dhali Boro	2249	Sylhet
DB4	Dholi Boro	180	Tangail
DB5	Dhali Boro	2245	Sylhet
DB6	Dholi Boro	4396	Sylhet
DB7	Dhali Boro	2246	Sylhet
DB8	Dhali Boro	2244	Sylhet
DB9	Dhali Boro	2248	Sylhet
DB10	Dhali Boro	2243	Sylhet

Serial No.	Accession name	Accession No. ^a	Origin
KS1	Kartik Sail	3243	Sylhet
KS2	Kartik Sail	776	Chittagong
KS3	Katih Shail	438	Rajshahi
KS4	Kartik Sail	539	Rangpur
KS5	Kartik Sail	77	Dhaka
KS6	Kati Shail	170	Tangail
KS7	Kartik Sail	3662	Sherpur
KS8	Kati Shail	3631	Rajshahi
KS9	Kartika	4053	Sylhet
KS10	Kartik Sail	4881	Tangail
KS11	Kartik Sail	76	Dhaka
KS12	Kati Shail	437	Rajshahi
KS13	Kartik Sail	78	Dhaka
KS14	Kartik Sail	1882	Kishorganj
KS15	Kartik sail (2)	689	Comilla
KS16	Kartik sail (2)	846	Sylhet
KS17	Kartik Sail	664	Comilla
KS18	Kartik Sail	1887	Kishorganj
KS19	Kartik Sail	844	Sylhet
KS20	Katih Shail	994	Khulna
KS21	Kartik Sail	845	Sylhet
DB1	Dhali Boro	2250	Sylhet
DB2	Dhali Boro	2247	Sylhet
DB3	Dhali Boro	2249	Sylhet
DB4	Dholi Boro	180	Tangail
DB5	Dhali Boro	2245	Sylhet
DB6	Dholi Boro	4396	Sylhet
DB7	Dhali Boro	2246	Sylhet
DB8	Dhali Boro	2244	Sylhet
DB9	Dhali Boro	2248	Sylhet
DB10	Dhali Boro	2243	Sylhet

Cluster	No. of genotypes	Serial No.
I	11	KS1, KS2, KS5, KS7, KS9, KS11, KS14, KS17, KS18, KS19, KS21
II	3	KS6, KS8, KS13
III	7	KS3, KS4, KS10, KS12, KS15, KS16, KS20
IV	10	DB1, DB2, DB3, DB4, DB5, DB6, DB7, DB8, DB9, DB10

Cluster	No. of genotypes	Serial No.
I	11	KS1, KS2, KS5, KS7, KS9, KS11, KS14, KS17, KS18, KS19, KS21
II	3	KS6, KS8, KS13
III	7	KS3, KS4, KS10, KS12, KS15, KS16, KS20
IV	10	DB1, DB2, DB3, DB4, DB5, DB6, DB7, DB8, DB9, DB10

Character	Cluster I	Cluster II	Cluster III	Cluster IV	Vector I	Vector II	Combined ranking ^a
Seedling height (cm)	66	69.9	56.7	34.8	0.1093	-0.0729	9
Plant height (cm)	123.9	125.6	111.1	106.1	-0.1082	0.0332	12
Culm diameter (mm)	5.27	5.16	4.77	4.28	1.7199	-1.788	11
Panicle exsertion (mm)	0.64	0.53	0.44	6.13	-1.6266	-0.2472	18
Effective tillering number per hill	11.8	11	12.1	10.3	0.2065	0.2738	3
Days to maturity (d)	143	141	142	160	-0.2783	-0.1692	14
Primary branch number per panicle	10.6	9	9.1	7.1	0.847	0.1551	2
Secondary branch number per panicle	33.6	21.7	24.9	16.9	0.1115	0.24	6
Grain yield per panicle (g)	3.2	2.7	2.3	1.9	0.2634	-1.1171	17
Grain length (mm)	8.55	8.44	8.17	7.62	0.2474	-0.3449	13
Awn length (mm)	0.6	21.5	0	30.5	-0.0198	0.1587	7
1000-grain weight (g)	23.19	27.18	20.82	20.69	-0.0652	0.5106	4
Grain yield per hill (g)	22.42	21.28	22	17.28	-0.3271	-0.1641	15
Biological yield (g)	40.9	37	33.1	27.7	0.1759	0.2617	5
Milling rate (%)	68.2	65.3	69.9	68.7	0.0361	-0.633	16
Cooking time (min)	17	20	16.5	19.1	-0.4281	0.3856	10
Amylose content (%)	25.3	25.27	25.76	21.17	0.2999	-0.2623	8
Protein content (%)	7.65	9.03	7.92	9.12	0.8121	0.6391	1