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    1. Progress on Molecular Mechanism of Heat Tolerance in Rice
    Fu Yiwei, Wu Jiayelu, Wu Mingming, Ye Shenghai, Zhai Rongrong, Ye Jing, Zhu Guofu, Yu Faming, Lu Yanting, Zhang Xiaoming
    Rice Science    2024, 31 (6): 673-687.   DOI: 10.1016/j.rsci.2024.07.001
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    Rice (Oryza sativa L.) is a major food crop in China, and its high and stable yield is crucial for ensuring food security in the country. However, over the past few years, extreme weather events induced by global climate change have impacted rice growth. For example, the effects of heat stress on rice quality and yield have been significant. Therefore, it is fundamental to conduct in-depth research on the heat-tolerance mechanisms of rice and to cultivate superior new thermotolerant rice varieties. This review summarizes the adverse effects of high temperatures on rice growth at various stages, the heat-tolerance mechanisms in rice, and the heat-tolerance genes and QTLs that have been identified in recent years. We also discuss strategies to enhance the heat tolerance of rice, offering new insights for rice breeding research.

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    2. Does Financial Inclusion Influence Economic Efficiency of Rice Farming? Evidence from Ogun State, Nigeria
    Shakirat B. Ibrahim, Raheem O. Aminu, Aisha O. Arowolo, Opeyemi O. Okanlawon, Afeez A. Adegbola
    Rice Science    2024, 31 (6): 638-642.   DOI: 10.1016/j.rsci.2024.10.001
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    3. Effect of Germinated Brown Rice Supplementation on Prediabetes Markers: A 120-Day Dietary Intervention Study in Selected Female Groups
    Hemalatha Ganapathyswamy, Thirukkumar Subramani, Kanmani Kalaivanan, Adiyaman Pannerselvam, Amutha Sundararajan
    Rice Science    2025, 32 (1): 11-14.   DOI: 10.1016/j.rsci.2024.08.006
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    4. Next Generation Nutrition: Genomic and Molecular Breeding Innovations for Iron and Zinc Biofortification in Rice
    Kunhikrishnan Hemalatha Dhanyalakshmi, Reshma Mohan, Sasmita Behera, Uday Chand Jha, Debashis Moharana, Ahalya Behera, Sini Thomas, Preman Rejitha Soumya, Rameswar Prasad Sah, Radha Beena
    Rice Science    2024, 31 (5): 526-544.   DOI: 10.1016/j.rsci.2024.04.008
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    Global efforts to address malnutrition and hidden hunger, particularly prevalent in low- and middle-income countries, have intensified, with a focus on enhancing the nutritional content of staple crops like rice. Despite serving as a staple for over half of the world’s population, rice falls short in meeting daily nutritional requirements, especially for iron (Fe) and zinc (Zn). Genetic resources, such as wild rice species and specific rice varieties, offer promising avenues for enhancing Fe and Zn content. Additionally, molecular breeding approaches have identified key genes and loci associated with Fe and Zn accumulation in rice grains. This review explores the genetic resources and molecular mechanisms underlying Fe and Zn accumulation in rice grains. The functional genomics involved in Fe uptake, transport, and distribution in rice plants have revealed key genes such as OsFRO1, OsIRT1, and OsNAS3. Similarly, genes associated with Zn uptake and translocation, including OsZIP11 and OsNRAMP1, have been identified. Transgenic approaches, leveraging transporter gene families and genome editing technologies, offer promising avenues for enhancing Fe and Zn content in rice grains. Moreover, strategies for reducing phytic acid (PA) content, a known inhibitor of mineral bioavailability, have been explored, including the identification of low-PA mutants and natural variants. The integration of genomic information, including whole-genome resequencing and pan-genome analyses, provides valuable insights into the genetic basis of micronutrient traits and facilitates targeted breeding efforts. Functional genomics studies have elucidated the molecular mechanisms underlying Fe uptake and translocation in rice. Furthermore, transgenic and genome editing techniques have shown promise in enhancing Fe and Zn content in rice grains through the manipulation of key transporter genes. Overall, the integration of multi-omics approaches holds significant promise for addressing global malnutrition and hidden hunger by enhancing the nutritional quality of rice, thereby contributing to improved food and nutritional security worldwide.

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    5. Rice Cultivation under Film Mulching Can Improve Soil Environment and Be Beneficial for Rice Production in China
    Zhang Youliang, Zhu Kaican, Tang Yongqi, Feng Shaoyuan
    Rice Science    2024, 31 (5): 545-555.   DOI: 10.1016/j.rsci.2024.06.009
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    Rice cultivation under film mulching is an integrated management technology that can conserve water, increase soil temperature, improve yield, and enhance water and nitrogen use efficiencies. Despite these advantages, the system does have its drawbacks, such as soil organic matter reduction and microplastic pollution, which impede the widespread adoption of film mulching cultivation in China. Nonetheless, the advent of degradable film, controlled-release fertilizer, organic fertilizer, and film mulching machinery is promoting the development of rice film mulching cultivation. This review outlines the impact of rice cultivation under film mulching on soil moisture, soil temperature, soil fertility, greenhouse gas emissions, weed control, and disease and pest management. It also elucidates the mechanism of changes in rice growth, yield and quality, water use efficiency, and nitrogen use efficiency. This paper incorporates a review of published research articles and discusses some uncertainties and shortcomings associated with rice cultivation under film mulching. Consequently, prospective research directions for the technology of rice film mulching cultivation are outlined, and recommendations for future research into rice cultivation under film mulching are proposed.

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    6. Three-Line Hybrid Rice in China: Fifty Years of Sustained Improvement in Yield, Quality, and Stress Resistance
    Gong Junyi, Zhang Xiaobo, Zhang Jianfu, Zeng Bo, Zhang Xiaoqing, Xu Xia, Cheng Benyi, Hou Yuxuan, Xia Junhui, Wu Jianli, Yang Shihua, Cheng Shihua, Han Bin, Xie Huaan
    Rice Science    2026, 33 (4): 499-513.   DOI: 10.1016/j.rsci.2026.04.004
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    China pioneered the global commercial application of hybrid rice (Oryza sativa L.). Chinese scientists not only proved that heterosis can be realized in self-pollinating crops, a groundbreaking theoretical advance, but also developed commercial hybrid rice breeding into one of China’s most sophisticated agricultural technology models, making outstanding contributions to national food security. This study systematically reviews the 50-year developmental trajectory of officially approved three-line hybrid rice varieties at national and provincial levels in China, through analyzing the approval statistics of individual restorer lines and male sterile lines, the dynamic changes in the popularized planting area of major varieties, and the phenotypic variation in core agronomic traits including grain yield, grain quality, and disease/pest resistance. The paper comprehensively summarizes the remarkable progress, major achievements and practical experience accumulated over half a century of three-line hybrid rice breeding in China, and prospects future research directions for this system.

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    7. Biochar Decreases Soil Cadmium (Cd) Availability and Regulates Expression Levels of Cd Uptake/Transport-Related Genes to Reduce Cd Translocation in Rice
    Wang Han, Huang Qina, Zhang Yan, Shao Guosheng, Hu Yijun, Xu Youxiang
    Rice Science    2024, 31 (5): 494-498.   DOI: 10.1016/j.rsci.2024.04.004
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    8. Genome-Wide Discovery of Candidate Genes Associated with Cold Tolerance in Rice at Various Growth Stages
    Liu Jiajun, Wang Cuili, Sun Mingmao, Ma Xiaoding, Han Bing, Guo Xiaohong, Han Longzhi, Cui Di
    Rice Science    2025, 32 (2): 137-142.   DOI: 10.1016/j.rsci.2024.12.001
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    9. Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate
    Jeberlin Prabina Bright, Hemant S. Maheshwari, Sugitha Thangappan, Kahkashan Perveen, Najat A. Bukhari, Debasis Mitra, Riyaz Sayyed, Andrea Mastinu
    Rice Science    2025, 32 (1): 94-106.   DOI: 10.1016/j.rsci.2024.08.008
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    The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus (P) and potassium (K) solubilizing bacteria, enhancing the survival of plant growth-promoting rhizobacteria, and ultimately the survival of bacteria in the rhizosphere for better plant growth. A new biofilm-based formulation comprising the diazotrophic cyanobacteria Anabaena AMP2, P-solubilizing Bacillus megaterium var. phosphaticum PB1, and K-solubilizing Rhizobium pusense KRBKKM1 was tested for efficacy in rice. The growth medium with half-strength BG-11 medium supplemented with 3% glucose showed best for biofilm formation under in vitro conditions. Analysis of the methanolic extract of the cyanobacterial- bacterial biofilm (CBB) showed the activity of antioxidants, such as 2-methoxy phenol and pentadecane, which are proven to improve plant-microbe interactions and plant growth, respectively. Treatment of rice seeds with CBB extract at 100 mL/kg or 200 mL/kg showed significant enhancement in germination rate and seedling length. Therefore, a pot culture experiment with the CBB formulations was carried out, and different growth and yield parameters were recorded. Principal component analysis showed that plant growth, yield, soil dehydrogenase activity, and soil chlorophyll content were positively correlated with rice plants amended with vermiculite-based CBB at 2 kg/hm2 followed by a spray with aqueous CBB formulation at 5 mL/L at 15 and 30 d after rice transplanting grown with a 25% reduced level of nitrogen/phosphorus/potassium chemical fertilizers than the recommended dose. Further, Pearson correlation analysis showed that yield was positively correlated with soil dehydrogenase (r = 0.92**) and soil chlorophyll content (r = 0.96**). We concluded that CBB could be used as a novel biofilm-based bio-inoculant to increase rice productivity and crop fitness as a component in integrated nutrient management and sustainable organic farming strategies with reduced chemical fertilizers.

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    10. Efficient Breeding of Early-Maturing Rice Cultivar by Editing Hd6 via CRISPR/Cas9
    Chen Zhihui, Tao Yajun, Xu Yang, Wu Jingjing, Wang Fangquan, Li Wenqi, Jiang Yanjie, Fan Fangjun, Li Xia, Zhu Jianping, Zhu Qian-Hao, Yang Jie
    Rice Science    2024, 31 (6): 629-633.   DOI: 10.1016/j.rsci.2024.06.007
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    11. Improving Semi-Dried Brown Rice Noodle Quality via Mixed Fermentation of Lactobacillus and Yeast
    Luo Lijuan, Cheng Zixuan, Qiao Fan, Xiong Gangping, Liu Jun, Huang Qingming, Li Jiangtao, Lin Qinlu, Liu Chun
    Rice Science    2024, 31 (5): 489-493.   DOI: 10.1016/j.rsci.2024.06.005
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    12. OsELF3.1-OsCATA-Ghd7 Pathway Regulates Rice Heading
    Wu Zhaozhong, Zhong Zhengzheng, Xu Peng, Liu Ling, Wang Beifang, Yang Qinqin, Wen Xiaoxia, Ma Guifang, Luo Mili, Zhang Yingxin, Liu Qun’en, Peng Zequn, Zhan Xiaodeng, Cao Liyong, Cheng Shihua, Wu Weixun
    Rice Science    2025, 32 (5): 658-672.   DOI: 10.1016/j.rsci.2025.06.001
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    Rice, a critical global staple crop, relies heavily on heading date, a key agronomic trait marking the transition from vegetative to reproductive growth. Understanding the genetic regulation of heading date is vital for enhancing the adaptability of high-quality rice varieties across diverse geographical regions and for bolstering local food security. In this study, we uncovered a novel role for OsCATA, a catalase gene, in the regulation of photoperiodic flowering in rice. We identified a novel allele of OsELF3.1, whose mutation resulted in delayed heading. Further analyses revealed that OsELF3.1 physically interacted with OsCATA. Notably, OsCATA exhibited rhythmic expression patterns similar to OsELF3.1 and, when mutated, also delayed flowering. Expression analyses showed that the delayed heading phenotype could be attributed to elevated Ghd7 expression under both long-day and short-day conditions, with OsCATA expression positively regulated by OsELF3.1. Double mutants of OsELF3.1 and OsCATA displayed a heading delay similar to that of oself3.1 single mutants. Additionally, OsELF3.1 could interact with Ghd7 in vivo, alleviating its suppression of Ehd1. Luciferase assays confirmed that Ghd7 repressed Ehd1 expression, while OsELF3.1 mitigated this repression. Collectively, our findings reveal that OsCATA is critical in suppressing Ghd7 expression through the OsELF3.1-OsCATA-Ghd7 transcriptional pathway, thereby regulating rice heading.

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    13. Identification of Sucrose Transporter (SUT) Genes Regulating Rice Yield and Quality
    Duan Yingqing, Li Xiaoxue, Wu Yawen, Jiao Guiai, Ma Liuyang, Dong Nannan, Chen Pengfei, Li Xinwei, Cao Ruijie, Chen Tianxiao, Hu Peisong, Wei Xiangjin
    Rice Science    2025, 32 (3): 287-291.   DOI: 10.1016/j.rsci.2025.01.003
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    14. Advances in Understanding Cadmium Stress and Breeding of Cadmium-Tolerant Crops
    Liang Liang, Wang Chenchang, Chen Tao
    Rice Science    2024, 31 (5): 507-525.   DOI: 10.1016//j.rsci.2024.06.006
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    Cadmium (Cd) pollution has emerged as a critical global environmental concern, due to its significant toxicity, environmental persistence, and the pervasiveness of contamination. Significantly, the bioaccumulation of Cd in agricultural crops constitutes a primary vector for its entry into the human diet. This issue warrants urgent attention from both the scientific community and policymakers to develop and implement effective mitigation strategies. This review delves into the physiological impacts of Cd stress on plants, including the suppression of photosynthetic activity, amplification of oxidative stress, and disruptions in mineral nutrient homeostasis. Additionally, the resistance mechanisms deployed by plants in response to Cd stress have been explored, and the prospective contributions of molecular breeding strategies in augmenting crop tolerance to Cd and minimizing its bioaccumulation have been assessed. By integrating and analyzing these findings, we seek to inform future research trajectories and proffer strategic approaches to enhance agricultural sustainability, safeguard human health, and protect environmental integrity.

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    15. Sustainable Management Strategies for Rice Leaffolder, Cnaphalocrocis medinalis (Guenée): Progress and Prospects
    Yang Yajun, Lu Yanhui, Tian Junce, Zheng Xusong, Guo Jiawen, Liu Xiaowei, Lü Zhongxian, Xu Hongxing
    Rice Science    2025, 32 (3): 322-338.   DOI: 10.1016/j.rsci.2024.12.011
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    The rice leaffolder, Cnaphalocrocis medinalis (Guenée), is a major migratory insect pest in paddy fields that damages rice by folding and feeding on leaves, causing chlorophyll loss and resulting in significant yield losses when its population density exceeds an economic threshold. Sustainable pest management requires ‘green plant protection’ solutions. Advances in science and technology have introduced numerous green methods for sustainable management of the rice leaffolder. This paper reviews recent research advancements in rice leaffolder management, such as ecological regulation, healthy cultivation, behavioral regulation, biological control, and rational insecticide application. Based on accurate monitoring and early warning systems, rice leaffolder management can incorporate comprehensive green control products, green control technologies, and control modes. This paper provides prospects for discussing the future of rice leaffolder management, achieving sustainable management of the rice leaffolder, and ensuring rice production safety.

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    16. HAG1 Regulates Arsenic Allocation and Accumulation in Rice Grains
    Yang Huan, Yu Jing, Xie Manyi, Xu Ping, Cao Zhenzhen, Chen Mingxue, Cao Zhaoyun
    Rice Science    2025, 32 (1): 1-5.   DOI: 10.1016/j.rsci.2024.08.003
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    17. RISE Method Based on Rare Allele Infusion and Sanger Sequencing Estimation: A Simple, Cheap, and Efficient Method for Detecting Transgene Copy Number in Rice
    Liu Tingchang, Huang Lifang, Liu Peng, Cui Yanchun, Chen Caiyan, Mao Donghai
    Rice Science    2024, 31 (5): 499-502.   DOI: 10.1016/j.rsci.2024.05.001
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    18. Compound Microbial Agent Improves Soil Redox Status to Reduce Methane Emissions from Paddy Fields
    Tao Yi, Xiao Deshun, Ye Chang, Liu Kancheng, Tang Xinxin, Ma Hengyu, Chu Guang, Yu Kai, Xu Chunmei, Wang Danying
    Rice Science    2024, 31 (6): 740-750.   DOI: 10.1016/j.rsci.2024.05.002
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    Paddy fields are considered a major source of methane (CH4) emissions. Aerobic irrigation methods have proven to be efficacious in mitigating CH4 emissions in paddy cultivation. The promising role of compound microbial agents in refining the rhizospheric ecosystem suggests their potential as novel agents in reducing CH4 emissions from paddy fields. To explore a new method of using compound microbial agents to reduce CH4 emissions, we conducted pot and field experiments over the period of 2022‒2023. We measured CH4 flux, the redox potential (Eh) of the soil, the concentration of dissolved oxygen (DO) in the floodwater, and the gene abundance of both methanogens (mcrA) and methanotrophs (pmoA). The results showed that the application of the compound microbial agent led to a significant increase in the DO levels within the floodwater and an increase of 9.26% to 35.01% in the Eh of the tillage soil. Furthermore, the abundance of pmoA increased by 31.20%, while the mcrA/pmoA ratio decreased by 25.96% at the maximum tillering stage. Applying 45−75 kg/hm2 of the compound microbial agent before transplanting resulted in a reduction of cumulative CH4 emissions from paddy fields by 17.49% in single- cropped rice and 43.54% to 50.27% in double-cropped late rice during the tillering stage. Correlation analysis indicated that CH4 flux was significantly negatively correlated with pmoA gene abundance and soil Eh, and positively related to the mcrA/pmoA ratio. Additionally, soil Eh was significantly positively correlated with pmoA gene abundance, suggesting that paddy soil Eh indirectly affected CH4 flux by influencing the pmoA gene abundance. In conclusion, the pre-planting application of the compound microbial agent at a rate of 45‒75 kg/hm2 can enhance the Eh in the rhizosphere and increase the abundance of the pmoA gene, thereby reducing CH4 emissions from paddy fields during the tillering stage of rice growth.

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    19. Bulked Segregant RNA-Seq Analysis of Pollinated Pistils Reveals Genes Influencing Spikelet Fertility in Rice
    Kanokwan Kaewmungkun, Keasinee Tongmark, Sriprapai Chakhonkaen, Numphet Sangarwut, Theerachai Thanananta, Amorntip Muangprom
    Rice Science    2024, 31 (5): 556-571.   DOI: 10.1016/j.rsci.2024.06.001
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    Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F5:6 generation plants derived from inter-subspecific crosses (Nipponbare × KDML105) with low (LS) and high seed-setting rates (HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4‒5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes (DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS- specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene (CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall (OsPME35 and OsPLL9) and controlling later processes such as pollen-stigma adhesion (Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.

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    20. Immune Sensor Xa21 Regulates Bacterial Leaf Blight Infection in Seven Rice Cultivars from Myanmar
    Nay Chi Aye, Rizwana Begum Syed Nabi, Muhammad Shahid, Nkulu Kabange Rolly, Rupesh Tayade, Lee-Bong Choon, Adil Hussain, Byung-Wook Yun
    Rice Science    2024, 31 (6): 634-637.   DOI: 10.1016/j.rsci.2024.06.008
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