Analysis of QTLs for Flag Leaf Shape and Its Response to Elevated CO2 
in Rice (Oryza sativa)

Abstract

Abstract: To understand the responses of flag leaf shape in rice to elevated CO2 environment and their genetic
characteristics, quantitative trait loci (QTLs) for flag leaf shape in rice were mapped onto the molecular marker linkage map of
chromosome segment substitution lines (CSSLs) derived from a cross between a japonica variety Asominori and an indica
variety IR24 under free air carbon dioxide enrichment (FACE, 200 μmol/mol above current levels) and current CO2
concentration (Ambient, about 370 μmol/mol). Three flag-leaf traits, flag-leaf length (LL), width (LW) and the ratio of LL to LW
(RLW), were estimated for each CSSL and their parental varieties. The differences in LL, LW and RLW between parents and
in LL and LW within IR24 between FACE and Ambient were significant at 1% level. The continuous distributions and
transgressive segregations of LL, LW and RLW were also observed in CSSL population, showing that the three traits were
quantitatively inherited under both FACE and Ambient. A total of 16 QTLs for the three traits were detected on
chromosomes 1, 2, 3, 4, 6, 8 and 11 with LOD (Log10-likelihood ratio) scores ranging from 3.0 to 6.7. Among them, four QTLs
(qLL-6*, qLL-8*, qLW-4*, and qRLW-6*) were commonly detected under both FACE and Ambient. Therefore, based on the
different responses to elevated CO2 in comparison with current CO2 level, it can be suggested that the expressions of several
QTLs associated with flag-leaf shape in rice could be induced by the high CO2 level.

Key words: rice (Oryza sativa), flag leaf, leaf shape character, quantitative trait locus, chromosome segment substitution lines, carbon dioxide enrichment

FAN Gui-zhi, DONG Yan-jun, WANG Chun-ming, WAN Jian-min, XIE Hui, XU Chang-liang, ZHU Jian-guo, CAI Qing-sheng. Analysis of QTLs for Flag Leaf Shape and Its Response to Elevated CO₂
in Rice (Oryza sativa)[J]. RICE SCIENCE, 2007, 14(1): 7-12 .

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Analysis of QTLs for Flag Leaf Shape and Its Response to Elevated CO₂
in Rice (Oryza sativa)

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