Characteristics of Resistance to Rice Sheath Blight of Zhongda 2, a Transgenic Rice Line as Modified by Gene “RC24”

Abstract

Abstract:

The transgenic rice, Zhongda 2, which was genetically modified from an indica rice line Zhuxian B by rice chitinase gene (RC24), had high resistance to rice sheath blight (Rhizoctonia solani) in laboratory and a two-year field experiment. The pathogen could invade sheath of Zhongda 2 and induce symptoms of the disease. No difference was noted in time of penetration or incubation period between Zhongda 2 and non-transgenic rice control, Zhuxian B, but the hyphae lysate could be observed earlier than control. Its resistance expressed as to inhibit the growth of mycelium in host tissue. F₁s from Zhongda 2 (♂) crossed with other five non-transgenic rice lines showed higher resistance than donor non-transgenic parents, but the resistance was different along with the different maternal parents.

Key words: transgenic rice, chitinase gene, rice sheath blight, resistance

YUAN Hong-xu , XU Xin-ping , ZHANG Jian-zhong , GUO Jian-fu , LI Bao-jian . Characteristics of Resistance to Rice Sheath Blight of Zhongda 2, a Transgenic Rice Line as Modified by Gene “RC24”
[J]. RICE SCIENCE.

References

1 Xu X P, Chen J F, Zhang J Z, Yang Q Y, Li B J. Novel trans-

genic rice strains resistant to blast and sheath blight. Acta Sci Nat Univ Sonyatse, 2001, 40(3): 131–134. (in Chinese with English abstract)

2 Zuo Q F, Zhang J Z, Yuan H X, Xu X P, Li B J. Breeding of transgenic rice Zhuzhuan 68 with blast resistance. Hybrid Rice, 2001, 16(1): 10–11. (in Chinese)

3 Pan X B, Chen Z X, Xu J Y, Tong Y H, Wang Z B, Pan X Y. The effects of different methods of inoculation and investigation on genetic research of resistance to rice sheath blight. J Jiangsu Agric Coll, 1997, 18(3): 27–32. (in Chinese with English abstract)

4 Broglie, K E, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais C J, Broglie R. Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science, 1991, 254: 1194–1197.

5 Bowles D J. Defense-related proteins in higher plants. Ann Rev Biochem, 1990, 59: 887–907.

6 Nielsen K K, Bojsen K, Collinge D B, Mikkelsen J D. Induced resistance in sugar beet against Cercospora beticola: Induction by dichloroisonicotinic acid is independent of chitinase and ß-1,3-glucanase transcript accumulation. Physiol Mol Plant Pathol, 1994, 45: 89–99.

7 Lorito M, Woo S L, Garcia I, Colucci G, Harman G E, Pintor-Toro J A, Filippone E, Muccifora S, Lawrence C B, Zoina A, Scala F. Genes from mycoparasitic fungi, a source for improving plant resistance to fungal pathogens. Proc Natl Acad Sci USA, 1998, 95: 7860–7865.

8 Hua Z H, Huang D N. Genetic mode of exogenes in transgenic plants. Acta Bot Sin, 1999, 41(1): 1–5. (in Chinese with English abstract)

9 Oard J H, Linscombe S D, Braverman M P, Jodari F, Blouin D C, Leech M, Kohli A, Vain P, Cooley J C, Christou P. Development, field evaluation, and agronomic performance of transgenic herbicide resistant rice. Mol Breeding, 1996, 2: 359–368.

[1]	Li Chao, Li He, Zhang Xianduo, Yang Zhimin. A Pleiotropic Drug Resistance Family Protein Gene Is Required for Rice Growth, Seed Development and Zinc Homeostasis [J]. Rice Science, 2023, 30(2): 127-137.
[2]	Liu Yantong, Li Ting, Jiang Zhishu, Zeng Chuihai, He Rong, Qiu Jiao, Lin Xiaoli, Peng Limei, Song Yongping, Zhou Dahu, Cai Yicong, Zhu Changlan, Fu Junru, He Haohua, Xu Jie. Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding [J]. Rice Science, 2022, 29(6): 522-534.
[3]	Fabiano T. P. K. Távora, Anne Cécile Meunier, Aurore Vernet, Murielle Portefaix, Joëlle Milazzo, Henri Adreit, Didier Tharreau, Octávio L. Franco, Angela Mehta. CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae [J]. Rice Science, 2022, 29(6): 535-544.
[4]	Wang Chenjiaozi, Zhao Mei, Shu Canwei, Zhou Erxun. Three Genes Related to Trehalose Metabolism Affect Sclerotial Development of Rhizoctonia solani AG-1 IA, Causal Agent of Rice Sheath Blight [J]. Rice Science, 2022, 29(3): 268-276.
[5]	Liu Kai, Li Minjuan, Zhang Bin, Yin Xuming, Xia Xinjie, Wang Manling, Cui Yanchun. Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice [J]. Rice Science, 2022, 29(3): 257-267.
[6]	R. Abdul Fiyaz, D. Shivani, K. Chaithanya, K. Mounika, M. Chiranjeevi, G. S. Laha, B. C. Viraktamath, L. V. Subba Rao, R. M. Sundaram. Genetic Improvement of Rice for Bacterial Blight Resistance: Present Status and Future Prospects [J]. Rice Science, 2022, 29(2): 118-132.
[7]	Zhou Ying, Wan Tao, Yuan Bin, Lei Fang, Chen Meijuan, Wang Qiong, Huang Ping, Kou Shuyan, Qiu Wenxiu, Liu Li. Improving Rice Blast Resistance by Mining Broad-Spectrum Resistance Genes at Pik Locus [J]. Rice Science, 2022, 29(2): 133-142.
[8]	Muduli Lakesh, Kumar Pradhan Sukanta, Mishra Abinash, Nath Bastia Debendra, Chandra Samal Kailash, Kumar Agrawal Pawan, Dash Manasi. Understanding Brown Planthopper Resistance in Rice: Genetics, Biochemical and Molecular Breeding Approaches [J]. Rice Science, 2021, 28(6): 532-546.
[9]	Yong Yang, Qiujun Lin, Xinyu Chen, Weifang Liang, Yuwen Fu, Zhengjin Xu, Yuanhua Wu, Xuming Wang, Jie Zhou, Chulang Yu, Chengqi Yan, Qiong Mei, Jianping Chen. Characterization and Proteomic Analysis of Novel Rice Lesion Mimic Mutant with Enhanced Disease Resistance [J]. Rice Science, 2021, 28(5): 466-478.
[10]	Saichompoo Uthomphon, Narumol Possawat, Nakwilai Pawat, Thongyos Peeranut, Nanta Aekchupong, Tippunya Patompong, Ruengphayak Siriphat, Itthisoponkul Teerarat, Bueraheng Niranee, Cheabu Sulaiman, Malumpong Chanate. Breeding Novel Short Grain Rice for Tropical Region to Combine Important Agronomical Traits, Biotic Stress Resistance and Cooking Quality in Koshihikari Background [J]. Rice Science, 2021, 28(5): 479-792.
[11]	Balija Vishalakshi, Bangale Umakanth, Ponnuvel Senguttuvel, Makarand Barbadikar Kalyani, Prasad Madamshetty Srinivas, Rao Durbha Sanjeeva, Yadla Hari, Madhav Maganti Sheshu. Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production [J]. Rice Science, 2021, 28(5): 493-500.
[12]	Silas Akos Ibrahim, Y. Rafii Mohd, Razi Ismail Mohd, Izan Ramlee Shairul, Abd Aziz Shamsudin Noraziyah, Ramli Asfaliza, Chibuike Chukwu Samuel, Swaray Senesie, Jalloh Momodu. Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines [J]. Rice Science, 2021, 28(3): 279-288.
[13]	Mishra Rukmini, Zheng Wei, Kumar Joshi Raj, Kaijun Zhao. Genome Editing Strategies Towards Enhancement of Rice Disease Resistance [J]. Rice Science, 2021, 28(2): 133-145.
[14]	Minghua Zhang, Zhaowen Mo, Juan Liao, Shenggang Pan, Xiongfei Chen, Le Zheng, Xiwen Luo, Zaiman Wang. Lodging Resistance Related to Root Traits for Mechanized Wet-Seeding of Two Super Rice Cultivars [J]. Rice Science, 2021, 28(2): 200-208.
[15]	Junhua Lu, Xuemei Yang, Jinfeng Chen, Tingting Li, Zijin Hu, Ying Xie, Jinlu Li, Jiqun Zhao, Mei Pu, Hui Feng, Jing Fan, Yanyan Huang, Jiwei Zhang, Wenming Wang, Yan Li. Osa-miR439 Negatively Regulates Rice Immunity Against Magnaporthe oryzae [J]. Rice Science, 2021, 28(2): 156-165.