Effects of Heat Stress at Vegetative and Reproductive Stages on Spikelet Fertility

doi:10.1016/j.rsci.2018.06.005

Abstract

Abstract:

Seed-setting rate, yield components and grain quality traits of 169 accessions from an exotic rice germplasm were tested under high temperatures from 40 ºC to 45 ºC for 6 h during the daytime at the vegetative and reproductive stages, respectively. The results showed that heat stress significantly decreased the seed-setting rate of all the accessions, but the heat stress effects varied among accessions. Based on the decreases in seed-setting rate at high temperatures, N22 was the most tolerant, followed by AUS17, M9962, SONALEE and AUS16. Moreover, the reductions in seed-setting rate and yield under heat stress were more serious at the vegetative stage (45 d before heading) than at the booting stage (15 d before heading). In addition, heat stress also affected grain quality, especially by conferring chalkiness to most of the accessions, but SONALEE did not change much. The heat-tolerant accessions identified here and the phenotype protocols developed could be used in future genetic studies and breeding programmes focused on heat tolerance.

Key words: heat tolerance, high temperature, rice germplasm, spikelet fertility, seed-setting rate

Cheabu Sulaiman, Moung-ngam Peerapon, Arikit Siwaret, Vanavichit Apichart, Malumpong Chanate. Effects of Heat Stress at Vegetative and Reproductive Stages on Spikelet Fertility[J]. Rice Science, 2018, 25(4): 218-226.

Figures/Tables 6

References 33

[1]	Adu-Kwarteng E, Ellis W O, Oduro I, Manful J T.2003. Rice grain quality: A comparison of local varieties with new varieties under study in Ghana.Food Control, 14(7): 507-514.
[2]	Asaoka M, Okuno K, Sugimoto Y, Kawakami J, Fuwa H.1984. Effect of environmental temperature during development of rice plants on some properties of endosperm starch.Starch Stärke, 36(6): 189-193.
[3]	Ceccarelli S, Grando S, Maatougui M, Michael M, Slash M, Haghparast R, Rahmanian M, Taheri A, Al-Yassin A, Benbelkacem A, Labdi M, Mimoun H, Nachit M.2010. Plant breeding and climate changes.J Agric Sci, 148(6): 627-637.
[4]	Che-abu S, Nat P, Rattanametta P, Vanavichit A, Malumpong C.2016. New Thai rice germplasm for heat tolerance M9962. In: The 4th Proceedings of the National Rice Conference. Bangkok, Thailand. 11-12 September.
[5]	Cao Y Y, Duan H, Yang L N, Wang Z Q, Liu L J, Zhang J H.2009. Effect of high temperature during heading and early filling on grain yield and physiological characteristics inindica rice. Acta Agron Sin, 35: 512-521.
[6]	Inouchi N, Ando H, Asaoka M, Okuno K, Fuwa H.2000. The effect of environmental temperature on distribution of unit chains of rice amylopectin.Starch Stärke, 52(1): 8-12.
[7]	International Rice Research Institute (IRRI). 2013. Standard Evaluation System for Rice (SES). Los Banos, the Philippines: International Rice Research Institute.
[8]	Ishimaru T, Hirabayashi H, Ida M, Takai T, San-Oh Y A, Yoshinaga S, Ando I, Ogawa T, Kondo M.2010. A genetic resource for early-morning flowering trait of wild riceOryza officinalis to mitigate high temperature-induced spikelet sterility at anthesis. Ann Bot, 106(3): 515-520.
[9]	Jagadish S V K, Craufurd P Q, Wheeler T R.2007. High temperature stress and spikelet fertility in rice (Oryza sativa L.). J Exp Bot, 58(7): 1627-1635.
[10]	Jagadish S V K, Craufurd P Q, Wheeler T R.2008. Phenotyping parents of mapping populations of rice for heat tolerance during anthesis.Crop Sci, 48(3): 1140-1146.
[11]	Jagadish S V K, Muthurajan R, Oane R, Wheeler T R, Heuer S, Bennett J, Craufurd P Q.2010. Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). J Exp Bot, 61(1): 143-156.
[12]	Kobayashi K, Matsui T, Murata Y, Yamamoto M.2011. Percentage of dehisced thecae and length of dehiscence control pollination stability of rice cultivarsat high temperatures.Plant Prod Sci, 14(2): 89-95.
[13]	Manigbas N L, Lambio L A F, Luvina B, Cardenas C C.2014. Germplasm innovation of heat tolerance in rice for irrigated lowland conditions in the Philippines.Rice Sci, 21(3): 162-169.
[14]	Maruyama A, Weerakoon W M W, Wakiyama Y, Ohba K.2013. Effects of increasing temperatures on spikelet fertility in different rice cultivars based on temperature gradient chamber experiments.J Agron Crop Sci, 199(6): 416-423.
[15]	Matsui T, Omasa K, Horie T.2001. The difference in sterility due to high temperatures during the flowering period amongjaponica-rice varieties. Plant Prod Sci, 4(2): 90-93.
[16]	Matsui T, Omasa K.2002. Rice (Oryza sativa L.) cultivars tolerant to high temperature at flowering: Anther characteristics. Ann Bot, 89(6): 683-687.
[17]	Mitsui T, Shiraya T, Kaneko K, Wada K.2013. Proteomics of rice grain under high temperature stress.Front Plant Sci, 4: 36.
[18]	Mittler R, Blumwald E.2010. Genetic engineering for modern agriculture: Challenges and perspectives.Ann Rev Plant Biol, 61: 443-462.
[19]	Mohammed A R, Tarpley L.2010. Effects of high night temperature and spikelet position on yield-related parameters of rice (Oryza sativa L.) plants. Eur J Agron, 33(2): 117-123.
[20]	Poli Y, Basava R K, Panigrahy M, Vinukonda V P, Dokula N R, Voleti S R, Desiraju S, Neelamraju S.2013. Characterization of a Nagina22 rice mutant for heat tolerance and mapping of yield traits.Rice, 6(1): 36.
[21]	Prasad P V V, Boote K J, Allen L H, Sheehy J E, Thomas J M G.2006. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress.Field Crops Res, 95(2): 398-411.
[22]	Rahman M A, Chikushi J, Yoshida S, Karim A J M S.2009. Growth and yield components of wheat genotypes exposed to high temperature stress under control environment.Bangl J Agric Res, 34(3): 360-372.
[23]	Satake T, Yoshida S.1978. High temperature-induced sterility in indica rices at flowering.Jpn J Crop Sci, 47(1): 6-17.
[24]	Shah F, Huang J L, Cui K H, Nie L M, Shah T, Chen C, Wang K.2011. Impact of high-temperature stress on rice plant and its traits related to tolerance.J Agric Sci, 149(5): 545-556.
[25]	Shi W, Ishimaru T, Gannaban R B, Oane W, Jagadish S V K.2015. Popular rice cultivars show contrasting responses to heat stress at gametogenesis and anthesis.Crop Sci, 55(2): 589-596.
[26]	Sowbhagya C M, Bhattacharya K R.1979. Simplified determination of amylose in milled rice.Starch Stärke, 31(5): 159-163.
[27]	Stocker T F, Qin D, Plattner G K, Tignor M, Allen S K, Boschung J, Nauels A, Xia Y, Bex V, Midgley P M.2014. Climate Change 2013: The Physical Science Basis. Cambridge, UK, and New York: Cambridge University Press.
[28]	Tanaka N, Fujita N, Nishi A, Satoh H, Hosaka Y, Ugaki M, Kawasaki S, Nakamura Y.2004. The structure of starch can be manipulated by changing the expression levels of starch branching enzyme IIb in rice endosperm.Plant Biotechnol J, 2(6): 507-516.
[29]	Tenorio F A, Ye C, Redona E, Sierra S, Laza M, Argayoso M A.2013. Screening rice genetic resources for heat tolerance.SABRAO J Breeding Genet, 45(3): 371-381.
[30]	Tsutsui K, Kaneko K, Hanashiro I, Nishinari K, Toshiaki M.2013. Characteristics of opaque and translucent parts of high temperature stressed grains of rice.J Appl Glycosci, 60(1): 61-67.
[31]	Ye C R, Argayoso M A, Redona E D, Sierra S N, Laza M A, Dilla C J, Mo Y J, Thomson M J, Chin J, Delaviña C B, Diaz G Q, Hernandez J E.2012. Mapping QTL for heat tolerance at flowering stage in rice using SNP markers.Plant Breeding, 131(1): 33-41.
[32]	Yin Y, Li S M, Liao W Q, Lu Q T, Wen X G, Lu C M.2010. Photosystem II photochemistry, photoinhibition, and the xanthophyll cycle in heat-stressed rice leaves.J Plant Physiol, 167(12): 959-966.
[33]	Yoshida S, Hara T.1977. Effects of air temperature and light on grain filling of anindica and a japonica rice(Oryza sativa L.) under controlled environmental conditions. Soil Sci Plant Nutr, 23(1): 93-107.

Country	HT	T	MT	S	Total
Bangladesh	2	1	2	2	7
Brazil	0	2	1	1	4
Cambodia	1	0	3	5	9
China	0	0	0	1	1
Guinea	0	0	1	0	1
India	3	8	16	14	41
Indonesia	0	5	3	3	11
Iran	0	0	2	0	2
Italy	0	0	1	0	1
Japan	0	0	0	1	1
Laos	0	0	0	1	1
Malaysia	1	3	1	0	5
Myanmar	0	2	0	2	4
Nepal	1	0	0	0	1
Nigeria	0	1	2	0	3
Pakistan	0	0	3	5	8
the Philippines	1	3	8	16	28
Senegal	0	1	1	1	3
South Korea	0	0	1	0	1
Sri Lanka	1	1	6	6	14
Suriname	0	0	0	1	1
Thailand	0	1	4	3	8
United States	0	2	2	0	4
Vietnam	0	1	2	7	10
Total	10 (5.9%)	31 (18.3%)	59 (34.9%)	69 (40.8%)	169
HT, Highly tolerant (seed-setting rate > 80.0%); T, Tolerant (seed-setting rate of 61.1%-80.0%); MT, Moderately tolerant (seed-setting rate of 40.1%-60.0%); S, Susceptible (seed-setting rate < 40.0%).

Country	HT	T	MT	S	Total
Bangladesh	2	1	2	2	7
Brazil	0	2	1	1	4
Cambodia	1	0	3	5	9
China	0	0	0	1	1
Guinea	0	0	1	0	1
India	3	8	16	14	41
Indonesia	0	5	3	3	11
Iran	0	0	2	0	2
Italy	0	0	1	0	1
Japan	0	0	0	1	1
Laos	0	0	0	1	1
Malaysia	1	3	1	0	5
Myanmar	0	2	0	2	4
Nepal	1	0	0	0	1
Nigeria	0	1	2	0	3
Pakistan	0	0	3	5	8
the Philippines	1	3	8	16	28
Senegal	0	1	1	1	3
South Korea	0	0	1	0	1
Sri Lanka	1	1	6	6	14
Suriname	0	0	0	1	1
Thailand	0	1	4	3	8
United States	0	2	2	0	4
Vietnam	0	1	2	7	10
Total	10 (5.9%)	31 (18.3%)	59 (34.9%)	69 (40.8%)	169
HT, Highly tolerant (seed-setting rate > 80.0%); T, Tolerant (seed-setting rate of 61.1%-80.0%); MT, Moderately tolerant (seed-setting rate of 40.1%-60.0%); S, Susceptible (seed-setting rate < 40.0%).

Accession	Genetic stock number	Origin	Seed-setting rate (%)			Total spikelet number per panicle
Accession	Genetic stock number	Origin	In field	Greenhouse	Decrease rate	In field	Greenhouse	Decrease rate (%)
N22 (CK)	12121	India	74.5	84.9	-	161	140	13
NP130	18118	Nepal	86.2	90.7	-	203	171	16
AUS16	18916	Bangladesh	88.1	84.9	4	160	111	30
MTU15	18117	India	86.8	82.9	5	174	158	9
AUS17	18915	Bangladesh	75.9	79.7	-	216	177	18
INDIA DULAR	18913	Brazil	78.4	77.8	1	171	160	6
DULAR(13703)	13703	India	84.9	74.5	12	146	159	-
DULAR(20018)	20018	India	86.7	74.0	15	143	149	-
CHEN KIN SEN KO	18911	Brazil	89.4	72.7	19	160	130	19
M9962	-	Thailand	70.0	71.2	-	142	151	-
SONALEE	4804	India	66.9	58.0	13	178	153	14

Accession	Genetic stock number	Origin	Seed-setting rate (%)			Total spikelet number per panicle
Accession	Genetic stock number	Origin	In field	Greenhouse	Decrease rate	In field	Greenhouse	Decrease rate (%)
N22 (CK)	12121	India	74.5	84.9	-	161	140	13
NP130	18118	Nepal	86.2	90.7	-	203	171	16
AUS16	18916	Bangladesh	88.1	84.9	4	160	111	30
MTU15	18117	India	86.8	82.9	5	174	158	9
AUS17	18915	Bangladesh	75.9	79.7	-	216	177	18
INDIA DULAR	18913	Brazil	78.4	77.8	1	171	160	6
DULAR(13703)	13703	India	84.9	74.5	12	146	159	-
DULAR(20018)	20018	India	86.7	74.0	15	143	149	-
CHEN KIN SEN KO	18911	Brazil	89.4	72.7	19	160	130	19
M9962	-	Thailand	70.0	71.2	-	142	151	-
SONALEE	4804	India	66.9	58.0	13	178	153	14