Rice Science ›› 2023, Vol. 30 ›› Issue (6): 577-586.DOI: 10.1016/j.rsci.2023.07.004

• Research Papers • Previous Articles     Next Articles

Novel QTLs from Wild Rice Oryza longistaminata Confer Strong Tolerance to High Temperature at Seedling Stage

Fan Fengfeng1,2, Cai Meng1, Luo Xiong1, Liu Manman1, Yuan Huanran1, Cheng Mingxing1, Ayaz Ahmad1, Li Nengwu1, Li Shaoqing1,2()   

  1. 1State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture and Rural Affairs, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan University, Wuhan 430072, China
    2Hubei Hongshan Laboratory, Wuhan 430072, China
  • Received:2023-04-25 Accepted:2023-07-03 Online:2023-11-28 Published:2023-08-10
  • Contact: Li Shaoqing (shaoqingli@whu.edu.cn)


Global warming poses a threat to rice production. Breeding heat-tolerant rice is an effective and economical approach to address this challenge. African rice is a valuable genetic resource for developing heat-tolerant crops due to its intricate mechanism for adapting to high temperatures. Oryza longistaminata, a widely distributed wild rice species in Africa, may harbor an even richer gene pool for heat tolerance, which remains untapped. In this study, we identified three heat tolerance QTLs from O. longistaminata at the seedling stage, including novel heat tolerance loci qTT4 and qTT5. Our findings demonstrated that the O. longistaminata alleles for these two QTLs can enhance the heat tolerance of rice seedlings. Remarkably, qTT5 was mapped to a region spanning approximately 287.2 kb, which contains 46 expressing genes. Through the analysis of Gene Ontology and expression differences under heat induction, we identified four candidate genes. Our results lay the foundation for discovering heat tolerance genes underlying O. longistaminata and developing new genetic resources for heat-tolerant rice breeding.

Key words: wild rice, Oryza longistaminata, heat tolerance, QTL