Antioxidant Protection Mechanisms Reveal Significant Response in Drought-Induced Oxidative Stress in Some Traditional Rice of Assam, India

doi:10.1016/j.rsci.2018.06.002

Abstract

Abstract:

Drought tolerance levels and antioxidant protection mechanisms were evaluated for 21 traditional rice varieties of Assam, India, along with Sahbhagi Dhan (drought tolerant) and IR64 (drought sensitive) as controls. Drought was imposed in hydroponic culture with polyethylene glycol 6000 (PEG6000) that was initially standardized with different concentrations. All the rice varieties showed apparent decreases in growth characteristics under drought stress (initially at 15% for 7 d followed by 20% PEG6000 for 7 d in Yoshida medium). On the basis of standard evaluation score (SES), eight rice varieties showed high drought tolerance which were carried forward for further biochemical analyses. Based on different morpho- physiological parameters, SN03 (Bora), SN04 (Prosad Bhog), SN05 (Kola Joha), SN06 (Helash Bora), SN08 (Salihoi Bao), SN12 (Kola Amona), SN20 (Ronga Bora) and SN21 (Sok-Bonglong) were identified as promising drought tolerant varieties. The non-enzymatic antioxidants activities viz., glutathione, ascorbate and enzymatic antioxidant activities such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), glutathione reductase (GR) in shoots and roots of all the selected varieties revealed significant level of protection mechanisms as compared with controls. Enhancement in activities of the overall antioxidant enzymes including SOD, GPX, CAT, GR and APX under drought stress reflects their role in the adaptation process under water stress.

Key words: drought stress, traditional rice, standard evaluation score, antioxidants protection mechanism, antioxidant enzyme

Nahar Shamsun, R. Vemireddy Lakshminarayana, Sahoo Lingaraj, Tanti Bhaben. Antioxidant Protection Mechanisms Reveal Significant Response in Drought-Induced Oxidative Stress in Some Traditional Rice of Assam, India[J]. Rice Science, 2018, 25(4): 185-196.

Figures/Tables 11

References 49

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Agro-climatic zone	Name and code of the collected rice
North Bank Plain Zone	Prosad Bhog (SN04), Dubori Bao (SN10), Sodiya Bao (SN09)
Upper Brahmaputra Valley Zone	Kola Joha (SN05), Helash Bora (SN06), Kola Kon Joha (SN07), Saliohoi Bao (SN08), Ronga Bao (SN11), Kola Amona (SN12), Deuri Lai (SN14), Makhan Bora (SN19), Ronga Bora (SN20)
Central Brahmaputra Valley Zone	Jahingia (SN17), Chokuwa (SN18), Kola Bao (SN13), Kola Ahu (SN15)
Lower Brahmaputra Valley Zone	IR64 IR64 (SN01), Sahbhagi Dhan (SN02), Bora (SN03)
Barak Valley Zone	Kola Sali (SN16)
Hill Zone	Sok-Bonglong (SN21), Amo (SN22), Halyan Amo (SN23)

Agro-climatic zone	Name and code of the collected rice
North Bank Plain Zone	Prosad Bhog (SN04), Dubori Bao (SN10), Sodiya Bao (SN09)
Upper Brahmaputra Valley Zone	Kola Joha (SN05), Helash Bora (SN06), Kola Kon Joha (SN07), Saliohoi Bao (SN08), Ronga Bao (SN11), Kola Amona (SN12), Deuri Lai (SN14), Makhan Bora (SN19), Ronga Bora (SN20)
Central Brahmaputra Valley Zone	Jahingia (SN17), Chokuwa (SN18), Kola Bao (SN13), Kola Ahu (SN15)
Lower Brahmaputra Valley Zone	IR64 IR64 (SN01), Sahbhagi Dhan (SN02), Bora (SN03)
Barak Valley Zone	Kola Sali (SN16)
Hill Zone	Sok-Bonglong (SN21), Amo (SN22), Halyan Amo (SN23)

Variety	GI	RL	SL	RFW	RDW	SFW	SDW	RWC	Chl a	Chl b	Total Chl	Total score	Rank
SN01	1.05	1.30	0.20	0.97	0.94	0.99	2.12	1.08	1.19	1.29	0.91	12.04	7
SN02	0.69	1.02	0.85	0.99	1.22	1.06	1.24	0.93	0.99	0.94	0.80	10.73	3
SN03	1.09	1.07	0.92	1.01	0.00	1.00	0.93	0.92	0.70	0.99	0.88	9.51	1
SN04	0.50	1.09	0.71	0.95	0.82	1.01	0.64	0.91	0.65	1.28	0.78	9.34	1
SN05	1.08	0.9	1.05	1.02	1.04	0.92	0.3	0.87	0.93	1.17	1.09	10.37	1
SN06	0.63	0.52	1.01	1.11	0.77	0.96	0.94	1.20	0.19	1.04	1.06	9.43	1
SN07	1.29	1.04	0.92	1.00	0.72	0.96	1.02	1.40	1.56	1.44	1.25	12.60	9
SN08	0.94	0.42	0.9	1.01	0.46	1.05	0.00	0.92	0.82	1.11	0.99	8.62	1
SN09	1.03	1.19	0.82	0.99	0.87	1.04	1.05	1.04	1.15	0.99	1.00	11.17	5
SN10	0.88	0.53	0.79	0.99	1.34	0.98	1.41	0.90	0.72	1.19	1.03	10.76	5
SN11	0.93	1.19	0.91	0.98	1.03	1.02	1.53	1.05	1.38	0.83	0.70	11.55	5
SN12	1.01	0.49	0.85	1.02	0.73	0.98	0.84	0.98	1.26	1.06	0.86	10.08	1
SN13	1.22	1.05	1.16	1.00	1.09	1.09	0.93	0.87	0.82	0.95	0.96	11.14	5
SN14	0.97	1.21	0.96	1.04	0.64	1.09	0.92	0.95	1.20	1.17	0.90	11.05	5
SN15	0.73	1.02	0.92	1.00	1.51	1.05	1.11	0.99	1.04	1.33	0.93	11.63	5
SN16	1.24	0.72	1.00	1.03	0.66	0.84	2.00	0.94	1.06	1.88	1.10	12.47	9
SN17	1.02	1.04	1.09	1.00	1.30	0.93	0.83	1.14	1.36	1.04	1.01	11.76	5
SN18	1.18	1.20	1.11	0.99	1.50	0.86	1.09	0.90	0.64	1.02	1.07	11.56	5
SN19	1.14	0.94	0.85	1.01	1.11	0.96	1.06	0.92	1.21	1.03	0.94	11.17	5
SN20	0.44	1.52	0.97	0.98	0.68	0.87	0.77	0.73	1.22	1.11	1.09	10.38	1
SN21	0.92	0.37	0.87	0.97	1.09	1.02	1.13	0.95	1.12	0.93	0.95	10.32	1
SN22	1.59	0.92	1.27	0.97	1.24	1.06	0.67	1.01	1.16	1.03	0.94	11.86	5
SN23	1.11	0.87	0.93	0.88	2.25	1.14	0.66	1.12	0.97	1.15	1.06	12.14	9
GI, Germination index; RL, Root length; SL, Shoot length; RFW, Root fresh weight; RDW, Root dry weight; SFW, Shoot fresh weight; SDW, Shoot dry weight; RWC, Relative water content; Chl, Chlorophyll.

Variety	GI	RL	SL	RFW	RDW	SFW	SDW	RWC	Chl a	Chl b	Total Chl	Total score	Rank
SN01	1.05	1.30	0.20	0.97	0.94	0.99	2.12	1.08	1.19	1.29	0.91	12.04	7
SN02	0.69	1.02	0.85	0.99	1.22	1.06	1.24	0.93	0.99	0.94	0.80	10.73	3
SN03	1.09	1.07	0.92	1.01	0.00	1.00	0.93	0.92	0.70	0.99	0.88	9.51	1
SN04	0.50	1.09	0.71	0.95	0.82	1.01	0.64	0.91	0.65	1.28	0.78	9.34	1
SN05	1.08	0.9	1.05	1.02	1.04	0.92	0.3	0.87	0.93	1.17	1.09	10.37	1
SN06	0.63	0.52	1.01	1.11	0.77	0.96	0.94	1.20	0.19	1.04	1.06	9.43	1
SN07	1.29	1.04	0.92	1.00	0.72	0.96	1.02	1.40	1.56	1.44	1.25	12.60	9
SN08	0.94	0.42	0.9	1.01	0.46	1.05	0.00	0.92	0.82	1.11	0.99	8.62	1
SN09	1.03	1.19	0.82	0.99	0.87	1.04	1.05	1.04	1.15	0.99	1.00	11.17	5
SN10	0.88	0.53	0.79	0.99	1.34	0.98	1.41	0.90	0.72	1.19	1.03	10.76	5
SN11	0.93	1.19	0.91	0.98	1.03	1.02	1.53	1.05	1.38	0.83	0.70	11.55	5
SN12	1.01	0.49	0.85	1.02	0.73	0.98	0.84	0.98	1.26	1.06	0.86	10.08	1
SN13	1.22	1.05	1.16	1.00	1.09	1.09	0.93	0.87	0.82	0.95	0.96	11.14	5
SN14	0.97	1.21	0.96	1.04	0.64	1.09	0.92	0.95	1.20	1.17	0.90	11.05	5
SN15	0.73	1.02	0.92	1.00	1.51	1.05	1.11	0.99	1.04	1.33	0.93	11.63	5
SN16	1.24	0.72	1.00	1.03	0.66	0.84	2.00	0.94	1.06	1.88	1.10	12.47	9
SN17	1.02	1.04	1.09	1.00	1.30	0.93	0.83	1.14	1.36	1.04	1.01	11.76	5
SN18	1.18	1.20	1.11	0.99	1.50	0.86	1.09	0.90	0.64	1.02	1.07	11.56	5
SN19	1.14	0.94	0.85	1.01	1.11	0.96	1.06	0.92	1.21	1.03	0.94	11.17	5
SN20	0.44	1.52	0.97	0.98	0.68	0.87	0.77	0.73	1.22	1.11	1.09	10.38	1
SN21	0.92	0.37	0.87	0.97	1.09	1.02	1.13	0.95	1.12	0.93	0.95	10.32	1
SN22	1.59	0.92	1.27	0.97	1.24	1.06	0.67	1.01	1.16	1.03	0.94	11.86	5
SN23	1.11	0.87	0.93	0.88	2.25	1.14	0.66	1.12	0.97	1.15	1.06	12.14	9
GI, Germination index; RL, Root length; SL, Shoot length; RFW, Root fresh weight; RDW, Root dry weight; SFW, Shoot fresh weight; SDW, Shoot dry weight; RWC, Relative water content; Chl, Chlorophyll.